Effects of L-arginine on refolding of lysine-tagged human insulin-like growth factor 1 expressed in Escherichia coli

Bioprocess and Biosystems Engineering

Volumn 35, Issue 1-2, 2012, Pages 255-263

  Choi, Seung Phill ^a   Park, Yong Cheol ^b   Lee, Jung Hwa ^c   Sim, Sang Jun ^a   Chang, Ho Nam ^d  

^a Korea University   (South Korea)

^b Kookmin University   (South Korea)

^c Korea University College of Medicine   (South Korea)

^d Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Inclusion body; Insulin like growth factor 1; L arginine; Refolding

Indexed keywords

3-DIMENSIONAL STRUCTURES; ANALYTICAL TOOL; CD SPECTROSCOPY; CHROMATOGRAPHIC PURIFICATION; DRY CELLS; E. COLI; FED-BATCH FERMENTATION; FUSION PROTEINS; INCLUSION BODIES; INSULIN-LIKE GROWTH FACTOR; INSULIN-LIKE GROWTH FACTOR 1; L-ARGININE; MALDI-TOF MASS SPECTROMETRY; POSITIVE EFFECTS; PROINSULIN; RECOMBINANT ESCHERICHIA COLI; REFOLDING; REFOLDING PROCESS; RP-HPLC; SELF AGGREGATION; SITE-SPECIFIC; THERAPEUTIC PROTEIN; UBIQUITIN; WESTERN BLOTTING;

CHROMATOGRAPHIC ANALYSIS; CIRCULAR DICHROISM SPECTROSCOPY; ESCHERICHIA COLI; FERMENTATION; INSULIN; MASS SPECTROMETRY; PEPTIDES; POSITIVE IONS;

AMINO ACIDS;

6 LYSINE TAG AND UBIQUITIN INSULIN LIKE GROWTH FACTOR 1 FUSION PROTEIN; ARGININE; HYBRID PROTEIN; RECOMBINANT SOMATOMEDIN C; UBIQUITIN; UNCLASSIFIED DRUG;

ALPHA HELIX; BACTERIAL STRAIN; CELL INCLUSION; CIRCULAR DICHROISM; CONFERENCE PAPER; CONTROLLED STUDY; ESCHERICHIA COLI; FED BATCH FERMENTATION; IN VITRO STUDY; ION EXCHANGE CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN CLEAVAGE; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN REFOLDING; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; WESTERN BLOTTING;

ARGININE; ESCHERICHIA COLI; GENETIC ENHANCEMENT; HUMANS; INSULIN-LIKE GROWTH FACTOR I; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; UBIQUITIN;

ESCHERICHIA COLI;

EID: 84857448424     PISSN: 16157591     EISSN: 16157605     Source Type: Journal    
DOI: 10.1007/s00449-011-0619-7     Document Type: Conference Paper

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