Sensitivity improvement in hydrophilic interaction chromatography negative mode electrospray ionization mass spectrometry using 2-(2-methoxyethoxy)ethanol as a post-column modifier for non-targeted metabolomics

Journal of Chromatography A

Volumn 1361, Issue , 2014, Pages 209-216

  Koch, Wendelin ^a,b,e   Forcisi, Sara ^a,b,e   Lehmann, Rainer ^c,d,e   Schmitt Kopplin, Philippe ^a,b,e  

^a German Research Center for Environment Health   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^d UNIVERSITY OF TÜBINGEN   (Germany)

^e GERMAN CENTER FOR DIABETES RESEARCH DZD   (Germany)

Author keywords

2 (2 Methoxyethoxy)ethanol; Dopant; Hydrophilic interaction chromatography; Metabolomics; Time of flight mass spectrometry; Ultra high pressure liquid chromatography

Indexed keywords

AMINO ACIDS; AMMONIA; CHEMICAL DETECTION; COLUMN CHROMATOGRAPHY; DOPING (ADDITIVES); DROPS; ELECTRODEPOSITION; ELECTROSPRAY IONIZATION; ETHANOL; HYDROPHILICITY; LIQUIDS; MASS SPECTROMETRY; MOLECULES; NEGATIVE IONS;

2-(2-METHOXYETHOXY)ETHANOL; HYDROPHILIC INTERACTION CHROMATOGRAPHY; METABOLOMICS; TIME OF FLIGHT MASS SPECTROMETRY; ULTRA HIGH PRESSURE LIQUID CHROMATOGRAPHY (UHPLC);

HIGH PRESSURE LIQUID CHROMATOGRAPHY;

ACETIC ACID; AMINO ACID; AMMONIUM ACETATE; ANION; DIETHYLENE GLYCOL MONOMETHYL ETHER; GLUTAMIC ACID; LYSINE; 2-(2-METHOXYETHOXY)ETHANOL; ALCOHOL;

ARTICLE; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; EVAPORATION; HUMAN; HYDROPHILIC INTERACTION CHROMATOGRAPHY; ISOTOPE LABELING; METABOLOMICS; NORMAL HUMAN; PARTICLE SIZE; SENSITIVITY ANALYSIS; TIME OF FLIGHT MASS SPECTROMETRY; URINALYSIS; ANALOGS AND DERIVATIVES; CHEMICAL PHENOMENA; CHEMISTRY; DEVICES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PHYSICAL CHEMISTRY; PROCEDURES;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ETHANOL; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; METABOLOMICS; PHYSICOCHEMICAL PROCESSES; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 84919701688     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2014.07.104     Document Type: Article

References (24)

1. Forcisi S., Moritz F., Kanawati B., Tziotis D., Lehmann R., Schmitt-Kopplin P. Liquid chromatography-mass spectrometry in metabolomics research: mass analyzers in ultra high pressure liquid chromatography coupling. J. Chromatogr. A 2013, 1292:51-65.
2. Schoenmakers P.J., van Molle S., Hayes C.M.G., Uunk L.G.M. Effects of pH in reversed-phase liquid chromatography. Anal. Chim. Acta 1991, 250:1-19.
3. Huang N., Siegel M.M., Kruppa G.H., Laukien F.H. Automation of a Fourier transform ion cyclotron resonance mass spectrometer for acquisition, analysis, and e-mailing of high-resolution exact-mass electrospray ionization mass spectral data. J. Am. Soc. Mass Spectrom.V 10 1999, 1166-1173.
4. Apffel Alex, Fischer Steven, Goldberg Gerson, Goodley Paul C., Kuhlmann F.E. Enhanced sensitivity for peptide mapping with electrospray liquid chromatography-mass spectrometry in the presence of signal suppression due to trifluoroacetic acid-containing mobile phases. J. Chromatogr. A 1995, 712:177-190.
5. Rütters H., Möhring T., Rullkötter J., Griep-Raming J., Metzger J.O. The persistent memory effect of triethylamine in the analysis of phospholipids by liquid chromatography/mass spectrometry. Rapid. Commun. Mass. Spectrom. 2000, 14:122-123.
6. Mallet C.R., Lu Z., Mazzeo J.R. A study of ion suppression effects in electrospray ionization from mobile phase additives and solid-phase extracts. Rapid Commun. Mass Spectrom. 2004, 18:49-58.
7. Wu Z., Gao W., Phelps M.A., Wu D., Miller D.D., Dalton J.T. Favorable effects of weak acids on negative-ion electrospray ionization mass spectrometry. Anal. Chem. 2004, 76:839-847.
8. Zhang X., Clausen M.R., Zhao X., Zheng H., Bertram H.C. Enhancing the power of liquid chromatography-mass spectrometry-based urine metabolomics in negative ion mode by optimization of the additive. Anal. Chem. 2012, 84:7785-7792.
9. Yanes O., Tautenhahn R., Patti G.J., Siuzdak G. Expanding coverage of the metabolome for global metabolite profiling. Anal. Chem. 2011, 83:2152-2161.
10. Ohmichi M., Yamaguchi Jun-ichi, Jingu Shigeji, Ogawa Naoyoshi, Higuchi Shohei Utility of postcolumn addition of 2-(2-methoxyethoxy)ethanol, a signal-enhancing modifier, for metabolite screening with liquid chromatography and negative ion electrospray ionization mass spectrometry. Anal. Chem. 1999, 71:5386-5390.
11. Nawrocki J. The silanol group and its role in liquid chromatography. J. Chromatogr. A 1997, 779:29-71.
12. Kloos D.P., Lingeman H., Niessen W.M., Deelder A.M., Giera M., Mayboroda O.A. Evaluation of different column chemistries for fast urinary metabolic profiling. J. Chromatogr. B: Analyt. Technol. Biomed. Life. Sci. 2013, 927:90-96.
13. Guo Y., Gaiki S. Retention behavior of small polar compounds on polar stationary phases in hydrophilic interaction chromatography. J. Chromatogr. A 2005, 1074:71-80.
14. Buszewski B., Noga S. Hydrophilic interaction liquid chromatography (HILIC)-a powerful separation technique. Anal. Bioanal. Chem. 2012, 402:231-247.
15. Wishart D.S., Jewison T., Guo A.C., Wilson M., Knox C., Liu Y., Djoumbou Y., Mandal R., Aziat F., Dong E., Bouatra S., Sinelnikov I., Arndt D., Xia J., Liu P., Yallou F., Bjorndahl T., Perez-Pineiro R., Eisner R., Allen F., Neveu V., Greiner R., Scalbert A. HMDB 3.0-the human metabolome database in 2013. Nucleic Acids Res. 2013, 41:D801-D807.
16. Wishart D.S., Knox C., Guo A.C., Eisner R., Young N., Gautam B., Hau D.D., Psychogios N., Dong E., Bouatra S., Mandal R., Sinelnikov I., Xia J., Jia L., Cruz J.A., Lim E., Sobsey C.A., Shrivastava S., Huang P., Liu P., Fang L., Peng J., Fradette R., Cheng D., Tzur D., Clements M., Lewis A., De Souza A., Zuniga A., Dawe M., Xiong Y., Clive D., Greiner R., Nazyrova A., Shaykhutdinov R., Li L., Vogel H.J., Forsythe I. HMDB: a knowledgebase for the human metabolome. Nucleic Acids Res. 2009, 37:D603-D610.
17. Wishart D.S., Tzur D., Knox C., Eisner R., Guo A.C., Young N., Cheng D., Jewell K., Arndt D., Sawhney S., Fung C., Nikolai L., Lewis M., Coutouly M.A., Forsythe I., Tang P., Shrivastava S., Jeroncic K., Stothard P., Amegbey G., Block D., Hau D.D., Wagner J., Miniaci J., Clements M., Gebremedhin M., Guo N., Zhang Y., Duggan G.E., Macinnis G.D., Weljie A.M., Dowlatabadi R., Bamforth F., Clive D., Greiner R., Li L., Marrie T., Sykes B.D., Vogel H.J., Querengesser L. HMDB: the human metabolome database. Nucleic Acids Res. 2007, 35:D521-D526.
18. Cech N.B., Enke C.G. Practical implications of some recent studies in electrospray ionization fundamentals. Mass. Spectrom. Rev. 2001, 20:362-387.
19. Cech N.B., Enke C.G. Effect of affinity for droplet surfaces on the fraction of analyte molecules charged during electrospray droplet fission. Anal. Chem. 2001, 73:4632-4639.
20. Grimm R.L., Beauchamp J.L. Evaporation and discharge dynamics of highly charged multicomponent droplets generated by electrospray ionization. J. Phys. Chem. A 2010, 114:1411-1419.
21. Wortmann A., Kistler-Momotova A., Zenobi R., Heine M.C., Wilhelm O., Pratsinis S.E. Shrinking droplets in electrospray ionization and their influence on chemical equilibria. J. Am. Soc. Mass. Spectrom. 2007, 18:385-393.
22. Wilm M. Principles of electrospray ionization. Mol. Cell. Proteomics 2011, 10(M111):009407.
23. Ekdahl A., Johansson M.C., Ahnoff M. Tracing and separating plasma components causing matrix effects in hydrophilic interaction chromatography-electrospray ionization mass spectrometry. J. Chromatogr. B: Analyt. Technol. Biomed. Life. Sci. 2013, 923-924C:83-91.
24. Polson C., Sarkar P., Incledon B., Raguvaran V., Grant R. Optimization of protein precipitation based upon effectiveness of protein removal and ionization effect in liquid chromatography-tandem mass spectrometry. J. Chromatogr. B 2003, 785:263-275.