One-step biosynthesis of α-keto-γ- Methylthiobutyric acid from l-methionine by an Escherichia coli whole-cell biocatalyst expressing an engineered lamino acid deaminase from proteus vulgaris

PLoS ONE

Volumn 9, Issue 12, 2014, Pages

  Hossain, Gazi Sakir ^a   Li, Jianghua ^a,b   Shin, Hyun Dong ^c   Du, Guocheng ^a,b   Wang, Miao ^a   Liu, Long ^a,b   Chen, Jian ^a,b  

^a JIANGNAN UNIVERSITY   (China)

^b Synergetic Innovation Center of Food Safety and Nutrition   (China)

^c GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 METHYLTHIO 2 OXOBUTYRIC ACID; ALANINE; AMINO ACID DEAMINASE; ARGININE; CALCIUM ALGINATE; DEAMINASE; LYSINE; METHIONINE; SERINE; UNCLASSIFIED DRUG; 2-KETO-4-METHYLTHIOBUTYRIC ACID; IMMOBILIZED ENZYME; LYASE;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL STRAIN; BACTERIUM MUTANT; BIOCATALYST; BIOSYNTHESIS; CATALYSIS; CELL DENSITY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; PH; PLASMID; POLYMERASE CHAIN REACTION; PROTEUS VULGARIS; TEMPERATURE; ANALOGS AND DERIVATIVES; BIOCATALYSIS; BIOTRANSFORMATION; CHEMISTRY; CYTOLOGY; DEAMINATION; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; GENE EXPRESSION; GENETICS; METABOLISM; PROTEIN ENGINEERING; PROTEIN TRANSPORT; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI; PROTEUS VULGARIS;

BIOCATALYSIS; BIOTRANSFORMATION; CLONING, MOLECULAR; DEAMINATION; DIRECTED MOLECULAR EVOLUTION; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; GENE EXPRESSION; LYASES; METHIONINE; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; PROTEIN TRANSPORT; PROTEUS VULGARIS; SUBSTRATE SPECIFICITY;

EID: 84919685899     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0114291     Document Type: Article

References (15)

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