A convenient method for europium-labeling of a recombinant chimeric relaxin family peptide R3/I5 for receptor-binding assays

Journal of Peptide Science

Volumn 19, Issue 6, 2013, Pages 350-354

  Zhang, Wei Jie ^a   Jiang, Qian ^a   Wang, Xin Yi ^a   Song, Ge ^a   Shao, Xiao Xia ^a   Guo, Zhan Yun ^a  

^a TONGJI UNIVERSITY   (China)

Author keywords

Europium labeling; Receptor binding assay; Recombinant expression; Relaxin family

Indexed keywords

EUROPIUM; RECOMBINANT RELAXIN;

ARTICLE; CIRCULAR DICHROISM; DRUG LABELING; DRUG RECEPTOR BINDING; PRIORITY JOURNAL;

EUROPIUM; HUMANS; INSULIN; PROTEIN BINDING; PROTEINS; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, PEPTIDE; RECOMBINANT PROTEINS; RELAXIN; STAINING AND LABELING;

EID: 84877711041     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.2507     Document Type: Article

References (19)

1. Shabanpoor F, Separovic F, Wade JD. The human insulin superfamily of polypeptide hormones. Vitam. Horm. 2009; 80: 1-31.
2. van der Westhuizen ET, Halls ML, Samuel CS, Bathgate RA, Unemori EN, Sutton SW, Summers RJ. Relaxin family peptide receptors--from orphans to therapeutic targets. Drug Discov. Today 2008; 3: 640-651.
3. Hsu SY, Nakabayashi K, Nishi S, Kumagai J, Kudo M, Sherwood OD, Hsueh AJ. Activation of orphan receptors by the hormone relaxin. Science 2002; 295: 671-674.
4. Sudo S, Kumagai J, Nishi S, Layfield S, Ferraro T, Bathgate RA, Hsueh AJ. H3 relaxin is a specific ligand for LGR7 and activates the receptor by interacting with both the ectodomain and the exoloop 2. J. Biol. Chem. 2003; 278: 7855-7862.
5. Kumagai J, Hsu SY, Matsumi H, Roh JS, Fu P, Wade JD, Bathgate RA, Hsueh AJ. INSL3/Leydig insulin-like peptide activates the LGR8 receptor important in testis descent. J. Biol. Chem. 2002; 277: 31283-31286.
6. Liu C, Eriste E, Sutton S, Chen J, Roland B, Kuei C, Farmer N, Jörnvall H, Sillard R, Lovenberg TW. Identification of relaxin-3/INSL7 as an endogenous ligand for the orphan G-protein-coupled receptor GPCR135. J. Biol. Chem. 2003; 278: 50754-50764.
7. Liu C, Kuei C, Sutton S, Chen J, Bonaventure P, Wu J, Nepomuceno D, Kamme F, Tran DT, Zhu J, Wilkinson T, Bathgate R, Eriste E, Sillard R, Lovenberg TW. INSL5 is a high affinity specific agonist for GPCR142 (GPR100). J. Biol. Chem. 2005; 280: 292-300.
8. Liu C, Chen J, Sutton S, Roland B, Kuei C, Farmer N, Sillard R, Lovenberg TW. Identification of relaxin-3/INSL7 as a ligand for GPCR142. J. Biol. Chem. 2003; 278: 50765-50770.
9. Selvin PR. Principles and biophysical applications of lanthanide-based probes. Annu. Rev. Biophys. Biomol. Struct. 2002; 31: 275-302.
10. Liu C, Chen J, Kuei C, Sutton S, Nepomuceno D, Bonaventure P, Lovenberg TW. Relaxin-3/insulin-like peptide 5 chimeric peptide, a selective ligand for G-protein coupled receptor (GPCR)135 and GPCR142 over leucine-rich repeat-containing G-protein coupled receptor 7. Mol. Pharmacol. 2005; 67: 231-240.
11. Shabanpoor F, Separovic F, Wade JD. General method for selective labelling of double-chain cysteine-rich peptides with a lanthanide chelate via solid-phase synthesis. J. Pept. Sci. 2011; 17: 169-173.
12. Zerhouni S, Amrani A, Nijs M, Smolders N, Azarkan M, Vincentelli J, Looze Y. Purification and characterization of papaya glutamine cyclotransferase, a plant enzyme highly resistant to chemical, acid and thermal denaturation. Biochim. Biophys. Acta 1998; 1387: 275-290.
13. Luo X, Bathgate RA, Zhang WJ, Liu YL, Shao XX, Wade JD, Guo ZY. Design and recombinant expression of insulin-like peptide 5 precursors and the preparation of mature human INSL5. Amino Acids 2010; 39: 1343-1352.
14. Luo X, Liu YL, Layfield S, Shao XX, Bathgate RA, Wade JD, Guo ZY. A simple approach for the preparation of mature human relaxin-3. Peptides 2010; 31: 2083-2088.
15. Zhang WJ, Luo X, Song G, Wang XY, Shao XX, Guo ZY. Design, recombinant expression and convenient A-chain N-terminal europium-labelling of a fully active human relaxin-3 analogue. FEBS J. 2012; 279: 1505-1512.
16. Zhang WJ, Gao XJ, Liu YL, Shao XX, Guo ZY. Design, recombinant preparation and europium-labeling of a fully active easily-labeled INSL3 analogue for receptor-binding assays. Process Biochem. 2012; 47: 1856-1860.
17. Zhang WJ, Luo X, Liu YL, Shao XX, Wade JD, Bathgate RA, Guo ZY. Site-specific DOTA/europium-labeling of recombinant human relaxin-3 for receptor-ligand interaction studies. Amino Acids 2012; 43: 983-992.
18. Luo X, Huang CH, Shao XX, Guo ZY. Design, recombinant expression and in vitro maturation of human insulin-like peptide 6 and a biotin-labeled analogue. Process Biochem. 2011; 46: 1243-1247.
19. Luo X, Bathgate RA, Liu YL, Shao XX, Wade JD, Guo ZY. Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3. FEBS J. 2009; 276: 5203-5211.