Preparation of high efficiency and low carry-over immobilized enzymatic reactor with methacrylic acid-silica hybrid monolith as matrix for on-line protein digestion

Journal of Chromatography A

Volumn 1371, Issue , 2014, Pages 48-57

  Yuan, Huiming ^a   Zhang, Lihua ^a   Zhang, Yukui ^a  

^a DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

Author keywords

Immobilized trypsin reactor; Low carry over protein digestion; One pot strategy; Organic inorganic hybrid monolith; Proteomics

Indexed keywords

AMMONIUM PERSULFATE; EFFICIENCY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHILICITY; MAMMALS; MASS SPECTROMETRY; PEPTIDES; PROTEOMICS; SILICA;

HEPATOCELLULAR CARCINOMA; IMMOBILIZED ENZYMATIC REACTORS; IMMOBILIZED TRYPSIN REACTORS; ONE POT; ORGANIC-INORGANIC HYBRID; PROTEIN DIGESTION; PROTEIN IDENTIFICATION; VINYLTRIMETHOXYSILANES;

ORGANIC-INORGANIC MATERIALS;

1 (3 DIMETHYLAMINOPROPYL) 3 ETHYLCARBODIIMIDE; AMMONIUM SULFATE; BACTERIAL PROTEIN; BOVINE SERUM ALBUMIN; ESCHERICHIA COLI PROTEIN; FUNCTIONAL GROUP; METHACRYLIC ACID; MYOGLOBIN; N HYDROXYSUCCINIMIDE; POLYETHYLENEIMINE; SILOXANE; TETRAMETHOXYSILANE; TRYPSIN; UNCLASSIFIED DRUG; VINYLTRIMETHOXYSILANE; IMMOBILIZED ENZYME; METHACRYLIC ACID DERIVATIVE; SILICON DIOXIDE;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ESCHERICHIA COLI; HYDROPHILICITY; IMMOBILIZED ENZYME REACTOR; LIVER CELL CARCINOMA; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; POLYMERIZATION; PROTEIN ANALYSIS; PROTEIN DEGRADATION; REVERSED PHASE LIQUID CHROMATOGRAPHY; ANIMAL; BOVINE; CHEMICAL PHENOMENA; CHEMISTRY; MASS SPECTROMETRY; METABOLISM; REPRODUCIBILITY; TANDEM MASS SPECTROMETRY;

BOVINAE; ESCHERICHIA COLI;

ANIMALS; CATTLE; CHROMATOGRAPHY, REVERSE-PHASE; ENZYMES, IMMOBILIZED; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; METHACRYLATES; MYOGLOBIN; REPRODUCIBILITY OF RESULTS; SERUM ALBUMIN, BOVINE; SILICON DIOXIDE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 84918540891     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2014.10.067     Document Type: Article

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