Integrated enzyme reactor and high resolving chromatography in "sub-chip" dimensions for sensitive protein mass spectrometry

Scientific Reports

Volumn 3, Issue , 2013, Pages

  Hustoft, Hanne Kolsrud ^a   Brandtzaeg, Ole Kristian ^a   Rogeberg, Magnus ^a,b   Misaghian, Dorna ^a   Torsetnes, Silje Bøen ^a   Greibrokk, Tyge ^a   Reubsaet, Léon ^a   Wilson, Steven Ray ^a   Lundanes, Elsa ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b AKERSHUS UNIVERSITY HOSPITAL   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; IMMOBILIZED ENZYME; PEPTIDE FRAGMENT; PRO-GASTRIN-RELEASING PEPTIDE (31-98); RECOMBINANT PROTEIN; TRYPSIN;

CHEMISTRY; HUMAN; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NANOTECHNOLOGY; PROCEDURES; PROTEOMICS;

BIOLOGICAL MARKERS; CHROMATOGRAPHY, LIQUID; ENZYMES, IMMOBILIZED; HUMANS; MASS SPECTROMETRY; NANOTECHNOLOGY; PEPTIDE FRAGMENTS; PROTEOMICS; RECOMBINANT PROTEINS; TRYPSIN;

EID: 84890583613     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep03511     Document Type: Article

References (38)

1. Surinova, S. et al. On the development of plasma protein biomarkers. J. Proteome Res. 10, 5-16, doi:10.1021/pr1008515 (2010).
2. Kim, J.-H., Inerowicz, D.,Hedrick, V.& Regnier, F. Integrated sample preparation methodology for proteomics: Analysis of native proteins. Anal. Chem. 85, 8039-8045, doi:10.1021/ac401477w (2013).
3. Apffel, A., Fischer, S., Goldberg, G., Goodley, P. C. & Kuhlmann, F. E. Enhanced sensitivity for peptide mapping with electrospray liquid chromatography-mass spectrometry in the presence of signal suppression due to trifluoroacetic acidcontaining mobile phases. J. Chromatogr. A 712, 177-190, doi:http://dx.doi.org/10.1016/0021-9673(95)00175-M (1995).
4. Gaspari, M. & Cuda, G. Nanoproteomics, Toms, S. A. & Weil, R. J. (ed.), 115-126 (Humana Press, New York, 2011).
5. Yin, H. & Killeen, K. The fundamental aspects and applications of Agilent HPLCChip. J. Sep. Sci. 30, 1427-1434, doi:10.1002/jssc.200600454 (2007). (Pubitemid 47082917)
6. Luo, Q., Gu, Y., Wu, S.-L., Rejtar, T. & Karger, B. L. Two-dimensional strong cation exchange/porous layer open tubular/mass spectrometry for ultratrace proteomic analysis using a 10 mm id poly(styrene-divinylbenzene) porous layer open tubular column with an on-line triphasic trapping column. Electrophoreisis 29, 1604-1611, doi:10.1002/elps. 200700741 (2008). (Pubitemid 351597517)
7. Luo, Q. et al. On-line 1D and 2D porous layer open tubular/LC-ESI-MS using 10-mm-i.d. poly(styrene 2 divinylbenzene) columns for ultrasensitive proteomic analysis. Anal.Chem. 79, 6174-6181, doi:10.1021/ac070583w (2007). (Pubitemid 47282328)
8. Yue, G., Luo, Q., Zhang, J., Wu, S.-L. & Karger, B. L. Ultratrace LC/MS proteomic analysis using 10-mm-i.d. porous layer open tubular poly(styrene 2 divinylbenzene) capillary columns. Anal. Chem. 79, 938-946, doi:10.1021/ac061411m (2006).
9. Rogeberg, M.,Wilson, S. R. G. Tyge & Lundanes, E. Separation of intact proteins on porous layer open tubular (PLOT) columns. J. Chromatogr. A 1217, 2782-2786 (2010).
10. Rogeberg, M. et al. Separation optimization of long porous layer open tubular columns for nano-LC-MS of limited proteomic samples. J. Sep. Sci. 36, 2838-2847, doi:10.1002/jssc.201300499 (2013).
11. Thakur, D. et al.Microproteomic analysis of 10,000 laser captured microdissected breast tumor cells using short-range sodium dodecyl sulfate-polyacrylamide gel electrophoresis and porous layer open tubular liquid chromatography tandem mass spectrometry. J. Chromatogr. A 1218, 8168-8174, doi:http://dx.doi.org/10. 1016/j.chroma.2011.09.022 (2011).
12. Wang, D., Hincapie, M., Rejtar, T. & Karger, B. L. Ultrasensitive characterization of site-specific glycosylation of affinity-purified haptoglobin from lung cancer patient plasma using 10 mm id porous layer open tubular liquid chromatography 2 linear ion trap collision-induced dissociation/electron transfer dissociation mass spectrometry. Anal. Chem. 83, 2029-2037 (2011).
13. Rogeberg, M., Malerod, H., Roberg-Larsen, H., Aass, C. & Wilson, S. R. On-line solid phase extraction-liquid chromatography, with emphasis on modern bioanalysis and miniaturized systems. J. Pharmaceut. Biomed. Anal. doi: http://dx. doi.org/10.1016/j.jpba.2013.05.006.
14. Massolini, G. & Calleri, E. Immobilized trypsin systems coupled on-line to separation methods: Recent developments and analytical applications. J. Sep. Sci. 28, 7-21, doi:10.1002/jssc.200401941 (2005). (Pubitemid 40214460)
15. Engel, L., Flemming, R., Johnson, T., Simpson, D.&Urh, M. Conveniently perform in-solution digestions with immobilized trypsin ,http://no.promega.com/resources/pubhub/conveniently-perform-in-solution- digestions-withimmobilized-trypsin/. (Date accessed: September 25th, 2013).
16. Rivera-Burgos, D. & Regnier, F. E. Native protein proteolysis in an immobilized enzyme reactor as a function of temperature. Anal. Chem. 84, 7021-7028, doi:10.1021/ac301114m (2012).
17. Rivera B. D. & Regnier, F. E. Disparities between immobilized enzyme and solution based digestion of transferrin with trypsin. J. Sep. Sci. 36, 454-460, doi:10.1002/jssc.201200740 (2013).
18. Abele, S., Smejkal, P., Yavorska, O., Foret, F. & Macka, M. Evanescent waveinitiated photopolymerisation as a new way to create monolithic open-tubular capillary columns: use as enzymatic microreactor for on-line protein digestion. Analyst 135, 477-481 (2010).
19. Nesterenko, E., Yavorska, O., Macka, M., Yavorskyy, A. & Paull, B. Monolithic porous layer open tubular (monoPLOT) columns for low pressure liquid chromatography of proteins. Anal. Methods 3, 537-543 (2011).
20. Huang, T., Mi, J. Q. & Zhang, X. X. Capillary electrochromatography of amino acids with a protein-bonded porous-layer open-tubular column. J. Sep. Sci. 29, 277-281, doi:10.1002/jssc.200500317 (2006).
21. Krenkova, J., Lacher, N. A. & Svec, F. Highly efficient enzyme reactors containing trypsin and endoproteinase lysC immobilized on porous polymer monolith coupled to MS suitable for analysis of antibodies. Anal. Chem. 81, 2004-2012 (2009).
22. Torsetnes, S. B.,Nordlund, M. S., Paus, E.,Halvorsen, T. G.&Reubsaet, L. Digging deeper into the field of the small cell lung cancer tumor marker ProGRP: A method for differentiation of its isoforms. J. Proteome Res. 12, 412-420, doi:10.1021/pr300751j (2012).
23. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. Basic local alignment search tool. J. Mol. Biol. 215, 403-410 (1990).
24. Hulthe, G., Petersson, M. A. & Fogelqvist, E. Coupling of open tubular liquid chromatography to electrospray mass spectrometry with a nanospray interface. Anal. Chem. 71, 2915-2921 (1999). (Pubitemid 29340485)
25. Wilm, M. & Mann, M. Analytical properties of the nanoelectrospray ion source. Anal. Chem. 68, 1-8, doi:10.1021/ac9509519 (1996).
26. Zhou,H., Ning, Z.,Wang, F., Seebun, D.& Figeys, D. Proteomic reactors and their applications in biology. FEBS Journal 278, 3796-3806, doi:10.1111/j.1742-4658.2011.08292.x (2011).
27. Krenkova, J. & Svec, F. Less common applications of monoliths: IV. Recent developments in immobilized enzyme reactors for proteomics and biotechnology. J. Sep. Sci. 32, 706-718, doi:10.1002/jssc.200800641 (2009).
28. Monzo, A., Sperling, E. & Guttman, A. Proteolytic enzyme-immobilization techniques for MS-based protein analysis. TrAC Trends in Analytical Chemistry 28, 854-864, doi:DOI: 10.1016/j.trac.2009.03.002 (2009).
29. Hustoft, H. K. et al. Integrative Proteomics Eastwood Leung, H. (ed.), 73-92 (InTech, Rijeka, 2012).
30. Ma, J., Zhang, L., Liang, Z., Zhang, W. & Zhang, Y. Recent advances in immobilized enzymatic reactors and their applications in proteome analysis. Anal. Chim. Acta 632, 1-8, doi:10.1016/j.aca.2007.08.045 (2009).
31. Kr?enková, J. & Foret, F. Immobilized microfluidic enzymatic reactors. Electrophoresis 25, 3550-3563, doi:10.1002/elps.200406096 (2004). (Pubitemid 40012112)
32. Nadler, T. et al.Automated proteolytic mapping of proteins. J. Chromatogr. A743, 91-98, doi:http://dx.doi.org/10.1016/0021-9673(96)00359-7 (1996). (Pubitemid 26316384)
33. Stigter, E. C. A., De, J. G. J.&van, B. W. P. Development of an open-tubular trypsin reactor for on-line digestion of proteins. Anal. Bioanal. Chem. 389, 1967-1977, doi:10.1007/s00216-007-1584-5 (2007). (Pubitemid 350231600)
34. Amankwa, L. N. & Kuhr, W. G. Trypsin-modified-fused-silica capillary microreactor for peptide mapping by capillary zone electrophoresis. Anal. Chem. 64, 1610-1613, doi:10.1021/ac00038a019 (1992).
35. Licklider, L. & Kuhr, W. G. Optimization of online peptide mapping by capillary zone electrophoresis. Anal. Chem. 66, 4400-4407, doi:10.1021/ ac00096a003 (1994). (Pubitemid 124006102)
36. Bao, H., Lui, T., Zhang, L. & Chen, G. Infrared-assisted proteolysis using trypsinimmobilized silica microspheres for peptide mapping. PROTEOMICS 9, 1114-1117, doi:10.1002/pmic.200800659 (2009).
37. Temporini, C. et al. Chymotrypsin immobilization on epoxy monolithic silica columns: Development and characterization of a bioreactor for protein digestion. J. Sep. Sci. 30, 3069-3076, doi:10.1002/jssc.200700337 (2007). (Pubitemid 350238342)
38. Lv, Y., Lin, Z. & Svec, F. Hypercrosslinked large surface area porous polymer monoliths for hydrophilic interaction liquid chromatography of small molecules featuring zwitterionic functionalities attached to gold nanoparticles held in layered structure. Anal. Chem. 84, 8457-8460, doi:10.1021/ac302438m (2012).