Vinyl functionalized silica hybrid monolith-based trypsin microreactor for on line digestion and separation via thiol-ene "click" strategy

Journal of Chromatography A

Volumn 1218, Issue 44, 2011, Pages 7982-7988

  Chen, Yingzhuang ^a,c   Wu, Minghuo ^b   Wang, Keyi ^a   Chen, Bo ^c   Yao, Shouzhuo ^a,c   Zou, Hanfa ^b   Nie, Lihua ^a  

^a HUNAN UNIVERSITY   (China)

^b DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

^c HUNAN NORMAL UNIVERSITY   (China)

Author keywords

Enzyme microreactor; On line digestion and separation; Silica hybrid monolith; Thiol ene "click" reaction; Vinyl functionalized

Indexed keywords

FUNCTIONALIZED; MICRO-REACTOR; ON-LINE DIGESTION; SILICA HYBRID MONOLITH; THIOL-ENES;

ARGININE; CAPILLARY ELECTROPHORESIS; COVALENT BONDS; OPTICAL PROJECTORS; POSITIVE IONS; REACTION RATES; SILICA; SOL-GEL PROCESS;

ENZYME IMMOBILIZATION;

N,N' METHYLENEBISACRYLAMIDE; SILICON DIOXIDE; THIOL DERIVATIVE; TOSYLARGININE METHYL ESTER; TRYPSIN; VINYL DERIVATIVE;

ARTICLE; BIOSENSOR; CAPILLARY ZONE ELECTROPHORESIS; CHEMICAL ANALYSIS; CHEMICAL REACTION; CHEMICAL STRUCTURE; CONTROLLED STUDY; DISULFIDE BOND; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; GRAVIMETRY; HYDROLYSIS; METHODOLOGY; MICHAELIS CONSTANT; MICROREACTOR; MOLECULAR WEIGHT; MORPHOLOGY; PHASE SEPARATION; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN METABOLISM; PURIFICATION; REACTOR; THERMOSTABILITY; ULTRASOUND;

ARGININE; BIOREACTORS; CHROMATOGRAPHY, LIQUID; CLICK CHEMISTRY; ENZYMES, IMMOBILIZED; METHACRYLATES; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, FLUORESCENCE; PEPTIDE FRAGMENTS; SILANES; SULFHYDRYL COMPOUNDS; TOSYLARGININE METHYL ESTER; TRYPSIN; VINYL COMPOUNDS;

EID: 80053970833     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.09.002     Document Type: Article

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