Epigallocatechin-3-gallate induces the apoptosis of hepatocellular carcinoma LM6 cells but not non-cancerous liver cells

International Journal of Molecular Medicine

Volumn 35, Issue 1, 2015, Pages 117-124

  Zhang, Yunjuan ^a   Duan, Wei ^b   Owusu, Lawrence ^a   Wu, Dachang ^a   Xin, Yi ^a  

^a DALIAN MEDICAL UNIVERSITY   (China)

^b DEAKIN UNIVERSITY   (Australia)

Author keywords

Apoptosis; Epigallocatechin 3 gallate; Hepatocellular carcinoma

Indexed keywords

CASPASE 3; CASPASE 9; CYTOCHROME C; EPIGALLOCATECHIN GALLATE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; PROTEIN BAX; PROTEIN BCL 2; PROTEIN P53; ANTINEOPLASTIC AGENT; APOPTOSIS REGULATORY PROTEIN; CATECHIN;

ANTINEOPLASTIC ACTIVITY; APOPTOSIS; APOPTOSIS ASSAY; ARTICLE; CANCER INHIBITION; CELL ACTIVITY; CELL VIABILITY ASSAY; CONTROLLED STUDY; DRUG DETERMINATION; DRUG MECHANISM; G1 PHASE CELL CYCLE CHECKPOINT; HEPATOCELLULAR CARCINOMA LM6 CELL LINE; HUMAN; HUMAN CELL; IN VITRO STUDY; LIVER CANCER CELL LINE; LIVER CELL; MITOCHONDRIAL MEMBRANE POTENTIAL; PROTEIN SECRETION; WESTERN BLOTTING; ANALOGS AND DERIVATIVES; CELL CYCLE; CELL SURVIVAL; DRUG EFFECTS; LIVER CELL CARCINOMA; METABOLISM; TUMOR CELL LINE;

ANTINEOPLASTIC AGENTS; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CARCINOMA, HEPATOCELLULAR; CATECHIN; CELL CYCLE; CELL LINE, TUMOR; CELL SURVIVAL; HEPATOCYTES; HUMANS; MEMBRANE POTENTIAL, MITOCHONDRIAL;

EID: 84917674153     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm.2014.1988     Document Type: Article

References (37)

1. El-Serag HB and Mason AC: Rising incidence of hepatocellular carcinoma in the United States. N Engl J Med 340: 745-750, 1999.
2. Parkin DM, Bray F, Ferlay J and Pisani P: Estimating the world cancer burden: Globocan 2000. Int J Cancer 94: 153-156, 2001.
3. lkai I, Yamaoka Y, Yamamoto Y, Ozaki N, Sakai Y, Satoh S, Shinkura N and Yamamoto M: Surgical intervention for patients with stage IV-A hepatocellular carcinoma without lymph node metastasis: proposal as a standard therapy. Ann Surg 227: 433-439, 1998.
4. Lee JS, Chu IS, Heo J, Calvisi DF, Sun Z, Roskams T, Durnez A, Demetris AJ and Thorgeirsson SS: Classification and prediction of survival in hepatocellular carcinoma by gene expression profiling. Hepatology 40: 667-676, 2004.
5. Song HY, Liu YK, Feng JT, Cui JF, Dai Z, Zhang LJ, Feng JX, Shen HL and Tang ZY: Proteomic analysis on metastasis-associated proteins of human hepatocellular carcinoma tissues. J Cancer Res Clin Oncol 132: 92-98, 2006.
6. Yang CS and Wang ZY: Tea and cancer. J Natl Cancer Inst 85: 1038-1049, 1993.
7. Balentine DA, Wiseman SA and Bouwens LC: The chemistry of tea flavonoids. Crit Rev Food Sci Nutr 37: 693-704, 1997.
8. Mukhtar H and Ahmad N: Green tea in chemoprevention of cancer. Toxicol Sci 52 (Suppl 2): 111-117, 1999.
9. Singh BN, Shankar S and Srivastava RK: Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications. Biochem Pharmacol 82: 1807-1821, 2011.
10. Thawonsuwan J, Kiron V, Satoh S, Panigrahi A and Verlhac V: Epigallocatechin-3-gallate (EGCG) affects the antioxidant and immune defense of the rainbow trout, Oncorhynchus mykiss. Fish Physiol Biochem 36: 687-697, 2010.
11. Mukhtar H and Ahmad N: Tea polyphenols: prevention of cancer and optimizing health. Am J Clin Nutr 71 (Suppl 6): 1698S-1702S, 2000.
12. Thangapazham RL, Singh AK, Sharma A, Warren J, Gaddipati J and Maheshwari RK: Green tea polyphenols and its constituent epigallocatechin gallate inhibits proliferation of human breast cancer cells in vitro and in vivo. Cancer Lett 245: 232-241, 2007.
13. Stuart EC, Scandlyn MJ and Rosengren RJ: Role of epigallocatechin gallate (EGCG) in the treatment of breast and prostate cancer. Life Sci 79: 2329-2336, 2006.
14. Ahmad N, Feyes DK, Nieminen AL, Agarwal R and Mukhtar H: Green tea constituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells. J Natl Cancer Inst 89: 1881-1886, 1997.
15. Masuda M, Suzui M and Weinstein IB: Effects of epigallocatechin-3-gallate on growth, epidermal growth factor receptor signaling pathways, gene expression, and chemosensitivity in human head and neck squamous cell carcinoma cell lines. Clin Cancer Res 7: 4220-4229, 2001.
16. Nihal M, Ahmad N, Mukhtar H and Wood GS: Anti-proliferative and proapoptotic effects of (-)-epigallocatechin-3-gallate on human melanoma: possible implications for the chemoprevention of melanoma. Int J Cancer 114: 513-521, 2005.
17. Gupta S, Hussain T and Mukhtar H: Molecular pathway for (-)-epigallocatechin-3-gallate-induced cell cycle arrest and apoptosis of human prostate carcinoma cells. Arch Biochem Biophys 410: 177-185, 2003.
18. Wei DZ, Yang JY, Liu JW and Tong WY: Inhibition of liver cancer cell proliferation and migration by a combination of (-)-epigallocatechin-3-gallate and ascorbic acid. J Chemother 15: 591-595, 2003.
19. Uesato S, Kitagawa Y, Kamishimoto M, Kumagai A, Hori H and Nagasawa H: Inhibition of green tea catechins against the growth of cancerous human colon and hepatic epithelial cells. Cancer Lett 170: 41-44, 2001.
20. Nishikawa T, Nakajima T, Moriguchi M, Jo M, Sekoguchi S, Ishii M, et al: A green tea polyphenol, epigalocatechin-3-gallate, induces apoptosis of human hepatocellular carcinoma, possibly through inhibition of Bcl-2 family proteins. J Hepatol 44: 1074-1082, 2006.
21. Wyllie AH, Kerr JF and Currie AR: Cell death: the significance of apoptosis. Int Rev Cytol 68: 251-306, 1980.
22. Soldatenkov VA and Smulson M: Poly (ADP-ribose) polymerase in DNA damage-response pathway: implications for radiation oncology. Int J Cancer 90: 59-67, 2000.
23. Wei MC, Zong WX, Cheng EH, Lindsten T, Panoutsakopoulou V, Ross AJ, et al: Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 292: 727-730, 2001.
24. Green DR and Reed JC: Mitochondria and apoptosis. Science 281: 1309-1312, 1998.
25. Marsden VS, O'Connor L, O'Reilly LA, Silke J, Metcalf D, Ekert PG, et al: Apoptosis initiated by Bcl-2-regulated caspase activation independently of the cytochrome c/Apaf-1/caspase-9 apoptosome. Nature 419: 634-637, 2002.
26. Tsujimoto Y: Cell death regulation by the Bcl-2 protein family in the mitochondria. J Cell Physiol 195: 158-167, 2003.
27. Kowaltowski AJ, Vercesi AE and Fiskum G: Bcl-2 prevents mitochondrial permeability transition and cytochrome c release via maintenance of reduced pyridine nucleotides. Cell Death Differ 7: 903-910, 2000.
28. Reed JC: Proapoptotic multidomain Bcl-2/Bax-family proteins: mechanisms, physiological roles, and therapeutic opportunities. Cell Death Differ 13: 1378-1386, 2006.
29. Schuler M, Bossy-Wetzel E, Goldstein JC, Fitzgerald P and Green DR: p53 induces apoptosis by caspase activation through mitochondrial cytochrome c release. J Biol Chem 275: 7337-7342, 2000.
30. Miyashita T and Reed JC: Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell 80: 293-299, 1995.
31. Ahmad N, Cheng P and Mukhtar H: Cell cycle dysregulation by green tea polyphenol epigallocatechin-3-gallate. Biochem Biophys Res Commun 275: 328-334, 2000.
32. Weinert TA and Hartwell LH: The RAD9 gene controls the cell cycle response to DNA damage in Saccharomyces cerevisiae. Science 241: 317-322, 1988.
33. Smerdon MJ, Kastan MB and Lieberman MW: Distribution of repair-incorporated nucleotides and nucleosome rearrangement in the chromatin of normal and xeroderma pigmentosum human fibroblasts. Biochemistry 18: 3732-3739, 1979.
34. Kastan MB, Canman CE and Christopher J: p53, cell cycle control and apoptosis: implications for cancer. Cancer Metastasis Rev 14: 3-15, 1995.
35. Chow HH, Cai Y, Hakim IA, Crowell JA, Shahi F, Brooks CA, Dorr RT, Hara Y and Alberts DS: Pharmacokinetics and safety of green tea polyphenols after multiple-dose administration of epigallocatechin gallate and polyphenon E in healthy individuals. Clin Cancer Res 9: 3312-3319, 2003.
36. Chow HH, Cai Y, Alberts DS, et al: Phase I pharmacokinetic study of tea polyphenols following single-dose administration of epigallocatechin gallate and polyphenon E. Cancer Epidemiol Biomarkers Prev 10: 53-58, 2001.
37. Li QS, Wang CY, Han GZ, Zou LL, Li L and Li N: Application of LC-MS/MS method in pharmacokinetic study of multi-components from tea polyphenols in rats. Chin J New Drugs 20: 817-823, 2011.