Transforming growth factor-β1-induced epithelial to mesenchymal transition increases mitochondrial content in the A549 non-small cell lung cancer cell line

Molecular Medicine Reports

Volumn 11, Issue 1, 2015, Pages 417-421

  Xu, Yunhua ^a   Lu, Shun ^a  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

Author keywords

Epithelial mesenchymal transition; Mitochondrial content; Non small cell lung cancer

Indexed keywords

CYTOCHROME C; EPIDERMAL GROWTH FACTOR; FIBRONECTIN; GENOMIC DNA; MITOCHONDRIAL DNA; MITOCHONDRIAL PROTEIN; NERVE CELL ADHESION MOLECULE; TRANSCRIPTION FACTOR SLUG; TRANSCRIPTION FACTOR SNAIL; TRANSCRIPTION FACTOR TWIST; TRANSFORMING GROWTH FACTOR BETA1; UVOMORULIN; VIMENTIN;

A549 CELL LINE; ARTICLE; CANCER CELL; CELL CONTACT; CELL POLARITY; CONTROLLED STUDY; COPY NUMBER VARIATION; DNA CONTENT; ENERGY METABOLISM; EPITHELIAL MESENCHYMAL TRANSITION; HUMAN; HUMAN CELL; MITOCHONDRIAL CONTENT; NON SMALL CELL LUNG CANCER; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION; DRUG EFFECTS; GENE DOSAGE; GENETICS; LUNG TUMOR; METABOLISM; MITOCHONDRION; PATHOLOGY; TUMOR CELL LINE;

CARCINOMA, NON-SMALL-CELL LUNG; CELL LINE, TUMOR; DNA, MITOCHONDRIAL; EPITHELIAL-MESENCHYMAL TRANSITION; GENE DOSAGE; HUMANS; LUNG NEOPLASMS; MITOCHONDRIA; TRANSFORMING GROWTH FACTOR BETA1;

EID: 84916238917     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2014.2678     Document Type: Article

References (41)

1. Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin 61: 69-90, 2011.
2. Dela Cruz CS, Tanoue LT and Matthay RA: Lung cancer: epidemiology, etiology and prevention. Clin Chest Med 32: 605-644, 2011.
3. Travis WD, Travis LB and Devesa SS: Lung cancer. Cancer 75: 191-202, 1995.
4. Kudo-Saito C, Shirako H, Takeuchi T and Kawakami Y: Cancer metastasis is accelerated through immunosuppression during Snail-induced EMT of cancer cells. Cancer Cell 15: 195-206, 2009.
5. Kalluri R and Weinberg RA: The basics of epithelial-mesen-chymal transition. J Clin Invest 119: 1420-1428, 2009.
6. Acloque H, Adams MS, Fishwick K, Bronner-Fraser M and Nieto MA: Epithelial-mesenchymal transitions: the importance of changing cell state in development and disease. J Clin Invest 119: 1438-1449, 2009.
7. Thiery JP, Acloque H, Huang RY and Nieto MA: Epithelial-mesenchymal transitions in development and disease. Cell 139: 871-890, 2009.
8. Zeisberg M and Neilson EG: Biomarkers for epithelial-mesen-chymal transitions. J Clin Invest 119: 1429-1437, 2009.
9. Peinado H, Olmeda D and Cano A: Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype? Nat Rev Cancer 7: 415-428, 2007.
10. Kumarswamy R, Mudduluru G, Ceppi P, et al: MicroRNA-30a inhibits epithelial-to-mesenchymal transition by targeting Snai1 and is downregulated in non-small cell lung cancer. Int J Cancer 130: 2044-2053, 2012.
11. Mani SA, Guo W, Liao MJ, et al: The epithelial-mesenchymal transition generates cells with properties of stem cells. Cell 133: 704-715, 2008.
12. Voulgari A and Pintzas A: Epithelial-mesenchymal transition in cancer metastasis: mechanisms, markers and strategies to overcome drug resistance in the clinic. Biochim Biophys Acta 1796: 75-90, 2009.
13. Arumugam T, Ramachandran V, Fournier KF, et al: Epithelial to mesenchymal transition contributes to drug resistance in pancreatic cancer. Cancer Res 69: 5820-5828, 2009.
14. Thomson S, Buck E, Petti F, et al: Epithelial to mesenchymal transition is a determinant of sensitivity of non-small-cell lung carcinoma cell lines and xenografts to epidermal growth factor receptor inhibition. Cancer Res 65: 9455-9462, 2005.
15. Yauch RL, Januario T, Eberhard DA, et al: Epithelial versus mesenchymal phenotype determines in vitro sensitivity and predicts clinical activity of erlotinib in lung cancer patients. Clin Cancer Res 11: 8686-8698, 2005.
16. Witta SE, Gemmill RM, Hirsch FR, et al: Restoring E-cadherin expression increases sensitivity to epidermal growth factor receptor inhibitors in lung cancer cell lines. Cancer Res 66: 944-950, 2006.
17. Maitah MY, Ali S, Ahmad A, Gadgeel S and Sarkar FH: Up-regulation of sonic hedgehog contributes to TGF-beta1-induced epithelial to mesenchymal transition in NSCLC cells. PLoS One 6: e16068, 2011.
18. Wang D, Su LY, Zhang AM, et al: Mitochondrial DNA copy number, but not haplogroup, confers a genetic susceptibility to leprosy in han chinese from southwest china. PLoS One 7: e38848, 2012.
19. Gianotti TF, Castano G, Gemma C, et al: Mitochondrial DNA copy number is modulated by genetic variation in the signal transducer and activator of transcription 3 (STAT3). Metabolism 60: 1142-1149, 2011.
20. Carling PJ, Cree LM and Chinnery PF: The implications of mito-chondrial DNA copy number regulation during embryogenesis. Mitochondrion 11: 686-692, 2011.
21. Moreno-Bueno G, Peinado H, Molina P, et al: The morphological and molecular features of the epithelial-to-mesenchymal transition. Nat Protoc 4: 1591-1613, 2009.
22. Fetisova EK, Avetisyan AV, Izyumov DS, Korotetskaya MV, Chernyak BV and Skulachev VP: Mitochondria-targeted anti-oxidant SkQR1 selectively protects MDR (Pgp 170)-negative cells against oxidative stress. FEBS letters 584: 562-566, 2010.
23. Venegas V, Wang J, Dimmock D and Wong LJ: Real-time quantitative PCR analysis of mitochondrial DNA content. Curr Protoc Hum Genet 68: 19.7.1-19.7.12, 2011.
24. Kasai H, Allen JT, Mason RM, Kamimura T and Zhang Z: TGF-beta1 induces human alveolar epithelial to mesenchymal cell transition (EMT). Respir Res 6: 56, 2005.
25. Pirozzi G, Tirino V, Camerlingo R, et al: Epithelial to mesen-chymal transition by TGFbeta-1 induction increases stemness characteristics in primary non small cell lung cancer cell line. PLoS One 6: e21548, 2011.
26. Tiwari N, Gheldof A, Tatari M and Christofori G: EMT as the ultimate survival mechanism of cancer cells. Semin Cancer Biol 22: 194-207, 2012.
27. Dingley S, Chapman KA and Falk MJ: Fluorescence-activated cell sorting analysis of mitochondrial content, membrane potential and matrix oxidant burden in human lymphoblastoid cell lines. Methods Mol Biol 837: 231-239, 2012.
28. Thiery JP: Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer 2: 442-454, 2002.
29. Pietila M, Palomaki S, Lehtonen S, et al: Mitochondrial function and energy metabolism in umbilical cord blood-and bone marrow-derived mesenchymal stem cells. Stem Cells Dev 21: 575-588, 2012.
30. Folmes CD, Nelson TJ, Dzeja PP and Terzic A: Energy metabolism plasticity enables stemness programs. Ann NY Acad Sci 1254: 82-89, 2012.
31. Diaz-Ruiz R, Uribe-Carvajal S, Devin A and Rigoulet M: Tumor cell energy metabolism and its common features with yeast metabolism. Biochim Biophys Acta 1796: 252-265, 2009.
32. Westermann B: Mitochondrial fusion and fssion in cell life and death. Nat Rev Mol Cell Biol 11: 872-884, 2010.
33. Falkenberg M, Larsson NG and Gustafsson CM: DNA replication and transcription in mammalian mitochondria. Annu Rev Biochem 76: 679-699, 2007.
34. Clay Montier LL, Deng JJ and Bai Y: Number matters: control of mammalian mitochondrial DNA copy number. J Genet Genomics 36: 125-131, 2009.
35. Bai RK, Perng CL, Hsu CH and Wong LJ: Quantitative PCR analysis of mitochondrial DNA content in patients with mito-chondrial disease. Ann NY Acad Sci 1011: 304-309, 2004.
36. Xing J, Chen M, Wood CG, et al: Mitochondrial DNA content: its genetic heritability and association with renal cell carcinoma. J Natl Cancer Inst 100: 1104-1112, 2008.
37. Blokhin A, Vyshkina T, Komoly S and Kalman B: Variations in mitochondrial DNA copy numbers in MS brains. J Mol Neurosci 35: 283-287, 2008.
38. Morten KJ, Ashley N, Wijburg F, et al: Liver mtDNA content increases during development: a comparison of methods and the importance of age-and tissue-specific controls for the diagnosis of mtDNA depletion. Mitochondrion 7: 386-395, 2007.
39. Tiao MM, Lin TK, Kuo F Y, et al: Early stage of biliary atresia is associated with signifcant changes in 8-hydroxydeoxyguanosine and mitochondrial copy number. J Pediatr Gastroenterol Nutr 45: 329-334, 2007.
40. Choi YS, Kim S and Pak YK: Mitochondrial transcription factor A (mtTFA) and diabetes. Diabetes Res Clin Pract 54 (Suppl 2): S3-S9, 2001.
41. Bai RK and Wong LJ: Simultaneous detection and quantifcation of mitochondrial DNA deletion(s), depletion and over-replication in patients with mitochondrial disease. J Mol Diagn 7: 613-622, 2005.