Engineering specificity in a dynamic protein complex with a single conserved mutation

FEBS Journal

Volumn 281, Issue 21, 2014, Pages 4892-4905

  Bashir, Qamar ^a,b   Meulenbroek, Elisabeth M ^a   Pannu, Navraj S ^a   Ubbink, Marcellus ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

^b UNIVERSITY OF THE PUNJAB   (Pakistan)

Author keywords

Cytochrome c; Cytochrome c peroxidase; Electron transport; Nuclear magnetic resonance; Protein ligand interactions; Specificity; Transient complex

Indexed keywords

CYTOCHROME C; CYTOCHROME C PEROXIDASE; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE PROTEIN; SPIN LABELING;

ARTICLE; BINDING AFFINITY; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME SPECIFICITY; HYDROGEN BOND; LIGAND BINDING; MUTATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTON NUCLEAR MAGNETIC RESONANCE; SINGLE CONSERVED MUTATION; SOLVATION; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; COMPARATIVE STUDY; GENETICS; HORSE; METABOLISM; MISSENSE MUTATION; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE; POINT MUTATION; PROTEIN ANALYSIS; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SPECIES DIFFERENCE; SPIN LABELING; STEREOISOMERISM; STRUCTURE ACTIVITY RELATION; X RAY CRYSTALLOGRAPHY;

EQUIDAE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CRYSTALLOGRAPHY, X-RAY; CYTOCHROME-C PEROXIDASE; CYTOCHROMES C; HORSES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION, MISSENSE; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; POINT MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN INTERACTION MAPPING; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SPECIES SPECIFICITY; SPIN LABELS; STEREOISOMERISM; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 84915746867     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.13028     Document Type: Article

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