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using procedures described in ref 12
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BD computations were done with MacroDox (see: Northrup, S. H.; Thomasson, K. A.; Miller, C. M.; Barker, P. D.; Eltis, L. D.; Guillemette, J. G.; Inglis, S. C.; Mauk, A. G. Biochemistry 1993, 32, 6613-6623. ) using procedures described in ref 12.
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70349086428
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Configurations were compared using Suppose, available through the Structural Biology Facility at Vanderbilt University
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Configurations were compared using Suppose, available through the Structural Biology Facility at Vanderbilt University.
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18
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19
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70349132559
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note
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20 was used as a template for Mb(+6), which was overexpressed in E. coli, purified, and reconstituted with Zn-deuteroporphyrin IX as in refs 10 and 11; complete characterization will be presented elsewhere. Flash photolysis employed LKS.60 (Applied Photophysics) equipped with a Xe-arc and a frequency-doubled Spectra-Physics INDI 40-10-HG Nd:YAG pulsed laser.
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20
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20144370426
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Hoffman, B. M.; Celis, L. M.; Cull, D. A.; Patel, A. D.; Seifert, J. L.; Wheeler, K. E.; Wang, J.; Yao, J.; Kurnikov, I. V.; Nocek, J. Proc. Natl. Acad. Sci. U.S.A. 2005, 702, 3564-3569.
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23
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70349113398
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note
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Preliminary kinetic measurements in viscous solutions suggest that even this ET rate constant involves conformational gating, and not just the ET process itself.
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