Corrigendum to “Dopamine and serotonin regulate tumor behavior by affecting angiogenesis” [Drug Resist. Updat. 17 (2014) 96–104] (Dopamine and serotonin regulate tumor behavior by affecting angiogenesis (2014) 17(4–6) (96–104), (S1368764614000508), (10.1016/j.drup.2014.09.001));Dopamine and serotonin regulate tumor behavior by affecting angiogenesis

Drug Resistance Updates

Volumn 17, Issue 4-6, 2014, Pages 96-104

  Peters, Marloes A M ^a   Walenkamp, Annemiek M E ^a   Kema, Ido P ^a   Meijer, Coby ^a   De Vries, Elisabeth G E ^a   Oosting, Sjoukje F ^a  

^a UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

Angiogenesis; Biogenic amines; Cancer; Dopamine; Serotonin

Indexed keywords

DOPAMINE; DOPAMINE 2 RECEPTOR; SEROTONIN; SEROTONIN RECEPTOR;

ANIMAL; CELL PROLIFERATION; ENDOTHELIUM CELL; HUMAN; METABOLISM; NEOPLASM; NEOVASCULARIZATION (PATHOLOGY); PATHOLOGY; PHYSIOLOGY; VASCULARIZATION;

ANIMALS; CELL PROLIFERATION; DOPAMINE; ENDOTHELIAL CELLS; HUMANS; NEOPLASMS; NEOVASCULARIZATION, PATHOLOGIC; RECEPTORS, DOPAMINE D2; RECEPTORS, SEROTONIN; SEROTONIN;

EID: 84914176279     PISSN: 13687646     EISSN: 15322084     Source Type: Journal    
DOI: 10.1016/j.drup.2020.100717     Document Type: Erratum

References (109)

1. G. Alpini, P. Invernizzi, and E. Gaudio Serotonin metabolism is dysregulated in cholangiocarcinoma, which has implications for tumor growth Cancer Res. 68 2008 9184 9193
2. C. Alvarez, L. Martí-Bonmatí, and E. Novella-Maestre Dopamine agonist cabergoline reduces hemoconcentration and ascites in hyperstimulated women undergoing assisted reproduction J. Clin. Endocrinol. Metab. 92 2007 2931 2937
3. M. Asada, S. Ebihara, and Y. Numachi Reduced tumor growth in a mouse model of schizophrenia, lacking the dopamine transporter Int. J. Cancer 123 2008 511 518
4. M. Asada, S. Ebihara, and S. Yamanda Depletion of serotonin and selective inhibition of 2B receptor suppressed tumor angiogenesis by inhibiting endothelial nitric oxide synthase and extracellular signal-regulated kinase 1/2 phosphorylation Neoplasia 11 2009 408 417
5. S. Basu, and P.S. Dasgupta Alteration of dopamine D2 receptors in human malignant stomach tissue Dig. Dis. Sci. 42 1997 1260 1264
6. S. Basu, and P.S. Dasgupta Decreased dopamine receptor expression and its second-messenger cAMP in malignant human colon tissue Dig. Dis. Sci. 44 1999 916 921
7. S. Basu, J.A. Nagy, and S. Pal The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability factor/vascular endothelial growth factor Nat. Med. 7 2001 569 574
8. S. Basu, C. Sarkar, and D. Chakroborty Ablation of peripheral dopaminergic nerves stimulates malignant tumor growth by inducing vascular permeability factor/vascular endothelial growth factor-mediated angiogenesis Cancer Res. 64 2004 5551 5555
9. J.M. Beaulieu, and R.R. Gainetdinov The physiology, signaling, and pharmacology of dopamine receptors Pharmacol. Rev. 63 2011 182 217
10. R. Bhattacharya, S. Sinha, and S.P. Yang The neurotransmitter dopamine modulates vascular permeability in the endothelium J. Mol. Signal. 3 2008 14
11. P.M. Brown, D.A. Drossman, and A.J.J. Wood The tryptophan hydroxylase inhibitor LX1031 shows clinical benefit in patients with nonconstipating irritable bowel syndrome Gastroenterology 141 2011 507 516
12. D. Campa, S. Zienolddiny, and H. Lind Polymorphisms of dopamine receptor/transporter genes and risk of non-small cell lung cancer Lung Cancer 56 2007 17 23
13. P. Carmeliet, and R.K. Jain Angiogenesis in cancer and other diseases Nature 407 2000 249 257
14. M.G. Cattaneo, E. Palazzi, G. Bondiolotti, and L.M. Vicentini 5-HT1D receptor type is involved in stimulation of cell proliferation by serotonin in human small cell lung carcinoma Eur. J. Pharmacol. 268 1994 425 430
15. S. Chakraborti, M. Mandal, S. Das, A. Mandal, and T. Chakraborti Regulation of matrix metalloproteinases: an overview Mol. Cell. Biochem. 253 2003 269 285
16. D. Chakroborty, C. Sarkar, R.B. Mitra, S. Banerjee, P.S. Dasgupta, and S. Basu Depleted dopamine in gastric cancer tissues: dopamine treatment retards growth of gastric cancer by inhibiting angiogenesis Clin. Cancer Res. 10 2004 4349 4356
17. D. Chakroborty, U.R. Chowdhury, C. Sarkar, R. Baral, P.S. Dasgupta, and S. Basu Dopamine regulates endothelial progenitor cell mobilization from mouse bone marrow in tumor vascularization J. Clin. Invest. 118 2008 1380 1389
18. D. Chakroborty, C. Sarkar, B. Basu, P.S. Dasgupta, and S. Basu Catecholamines regulate tumor angiogenesis Cancer Res. 69 2009 3727 3730
19. T.N. Chase Levodopa therapy: consequences of the nonphysiologic replacement of dopamine Neurology 50 5 Suppl. 5 1998 S17 S25
20. S.U. Chen, C.H. Chou, and C.W. Lin Signal mechanisms of vascular endothelial growth factor and interleukin-8 in ovarian hyperstimulation syndrome: dopamine targets their common pathways Hum. Reprod. 25 2010 757 767
21. S. Chevalier, I. Defoy, and J. Lacoste Vascular endothelial growth factor and signaling in the prostate: more than angiogenesis Mol. Cell. Endocrinol. 189 2002 169 179
22. A. Chiechi, C. Novello, and G. Magagnoli Elevated TNFR1 and serotonin in bone metastasis are correlated with poor survival following bone metastasis diagnosis for both carcinoma and sarcoma primary tumors Clin. Cancer Res. 19 2013 2473 2485
23. A.R. Cools, and M.A. Gingras Nijmegen high and low responders to novelty: a new tool in the search after the neurobiology of drug abuse liability Pharmacol. Biochem. Behav. 60 1998 151 159
24. L.A. Cornelius, L.C. Nehring, and E. Harding Matrix metalloproteinases generate angiostatin: effects on neovascularization J. Immunol. 161 1998 6845 6852
25. M. Da Prada, and A. Pletscher Differential uptake of biogenic amines by isolated 5-hydroxytryptamine organelles of blood platelets Life Sci. 8 1969 65 72
26. M. Da Prada, and G.B. Picotti Content and subcellular localization of catecholamines and 5-hydroxytryptamine in human and animal blood platelets: monoamine distribution between platelets and plasma Br. J. Pharmacol. 65 1979 653 662
27. P.S. Dasgupta, and T. Lahiri Antitumor effect of i.p. dopamine in mice bearing Ehrlich ascites carcinoma J. Cancer Res. Clin. Oncol. 113 1987 363 368
28. F. Delgado-Rosas, R. Gómez, and H. Ferrero The effects of ergot and non-ergot derived dopamine agonists in an experimental mouse model of endometriosis Reproduction 142 2011 745 755
29. A. Delvigne, and S. Rozenberg Epidemiology and prevention of ovarian hyperstimulation syndrome (OHSS): a review Hum. Reprod. Update 8 2002 559 577
30. S. Dimmeler, I. Fleming, and B. Fisslthaler Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation Nature 399 1999 601 605
31. N. Dizeyi, A. Bjartell, P. Hedlund, K.A. Taskén, V. Gadaleanu, and P.A. Abrahamsson Expression of serotonin receptors 2B and 4 in human prostate cancer tissue and effects of their antagonists on prostate cancer cell lines Eur. Urol. 47 2005 895 900
32. I. Drozdov, M. Kidd, and B.I. Gustafsson Autoregulatory effects of serotonin on proliferation and signaling pathways in lung and small intestine neuroendocrine tumor cell lines Cancer 115 2009 4934 4945
33. G. Eisenhofer, A. Aneman, and P. Friberg Substantial production of dopamine in the human gastrointestinal tract J. Clin. Endocrinol. Metab. 82 1997 3864 3871
34. G. Eisenhofer, M.W. Coughtrie, and D.S. Goldstein Dopamine sulphate: an enigma resolved Clin. Exp. Pharmacol. Physiol. Suppl. 26 1999 S41 S53
35. B.A. Faucheux, A.M. Bonnet, Y. Agid, and E.C. Hisch Blood vessels change in the mesencephalon of patients with Parkinson's disease Lancet 353 1999 981 982
36. P. Frankhauser, Y. Grimmer, P. Bugert, M. Deuschle, M. Schmidt, and P. Schloss Characterization of the neuronal dopamine transporter DAT in human blood platelets Neurosci. Lett. 399 2006 197 201
37. S. Franks Polycystic ovary syndrome N. Engl. J. Med. 333 1995 853 861
38. S. Ganguly, B. Basu, and S. Shome Dopamine, by acting through its D2 receptor, inhibits insulin-like growth factor-I (IGF-I)-induced gastric cancer cell proliferation via up-regulation of Krüppel-like factor 4 through down-regulation of IGF-IR and AKT phosphorylation Am. J. Pathol. 177 2010 2701 2707
39. F. Gemignani, S. Landi, and V. Moreno Polymorphisms of the dopamine receptor gene DRD2 and colorectal cancer risk Cancer Epidemiol. Biomarkers Prev. 14 2005 1633 1638
40. L.C. Giudice, and L.C. Kao Endometriosis Lancet 364 2004 1789 1799
41. R. Gomez, M. Gonzalez-Izquierdo, and R.C. Zimmermann Low-dose dopamine agonist administration blocks vascular endothelial growth factor (VEGF)-mediated vascular hyperpermeability without altering VEGF receptor 2-dependent luteal angiogenesis in a rat ovarian hyperstimulation model Endocrinology 147 2006 5400 5411
42. R. Gómez, S.R. Soares, C. Busso, J.A. García-Velasco, C. Símon, and A. Pellicer Physiology and pathology of ovarian hyperstimulation syndrome Semin. Reprod. Med. 28 2010 448 457
43. R. Gómez, A. Abad, F. Delgado, S. Tamarit, C. Simón, and A. Pellicer Effects of hyperprolactinemia treatment with the dopamine agonist quinagolide on endometriotic lesions in patients with endometriosis-associated hyperprolactinemia Fertil. Steril. 95 2011 882 888
44. R. Gómez, H. Ferror, and F. Delgado-Rosas Evidences for the existence of a low dopaminergic tone in polycystic ovarian syndrome: implications for OHSS development and treatment J. Clin. Endocrinol. Metab. 96 2011 2484 2492
45. D. Hanahan, and L.M. Coussens Accessories to the crime: functions of cells recruited to the tumor microenvironment Cancer Cell 21 2012 309 322
46. D. Hanahan, and R.A. Weinberg The hallmarks of cancer: the next generation Cell 144 2011 646 674
47. E. Heinrich, L. Trojan, and D. Friedrich Neuroendocrine tumor cells in prostate cancer: evaluation of the neurosecretory products serotonin, bombesin, and gastrin - impact on angiogenesis and clinical follow-up Prostate 71 2011 1752 1758
48. B. Heissig, K. Hattori, and S. Dias Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand Cell 109 2002 625 637
49. H. Holmsen, and H.J. Weiss Secretable storage pools in platelets Annu. Rev. Med. 30 1979 119 134
50. M. Ishibashi, M. Fujisawa, H. Furue, Y. Maeda, M. Fukuyama, and T. Yamaji Inhibition of growth of human small cell lung cancer by bromocriptine Cancer Res. 54 1994 3442 3446
51. J.E. Italiano Jr., J.L. Richardson, and S. Patel-Hett Angiogenesis is regulated by a novel mechanism: pro- and antiangiogenic proteins are organized into separate platelet alpha granules and differentially released Blood 111 2008 1227 1233
52. M. Iwabayashi, Y. Taniyama, and F. Sanada Role of serotonin in angiogenesis: induction of angiogenesis by sarpogrelate via endothelial 5-HT1B/Akt/eNOS pathway in diabetic mice Atherosclerosis 220 2012 337 342
53. R.K. Jain Normalization of tumor vasculature: an emerging concept in antiangiogenic therapy Science 307 2005 58 62
54. P.K. Kopparapu, M. Tinzl, L. Anagnostaki, J.L. Persson, and N. Dizeyi Expression and localization of serotonin receptors in human breast cancer Anticancer Res. 33 2013 363 370
55. I.J. Kopin Catecholamine metabolism: basic aspects and clinical significance Pharmacol. Rev. 37 1985 333 364
56. A.E. Lang, and A.M. Lozano Parkinson's disease. First of two parts N. Engl. J. Med. 339 1998 1044 1053
57. C. Liang, W. Chen, and X. Zhi Serotonin promotes the proliferation of serum-deprived hepatocellular carcinoma cells via upregulation of FOXO3a Mol. Cancer 12 2013 14
58. H.S. Lindgren, K.E. Ohlin, and M.A. Cenci Differential involvement of D1 and D2 dopamine receptors in l-DOPA-induced angiogenic activity in a rat model of Parkinson's disease Neuropsychopharmacology 34 2009 2477 2488
59. R.B. Livingston, U. Ambus, S.L. George, E.J. Freireich, and J.S. Hart In vitro determination of thymidine-3H labeling index in human solid tumors Cancer Res. 34 1974 1376 1380
60. N. Maleki-Dizaji, T. Eteraf-Oskouei, A. Fakhrjou, S.H. Maljaie, and A. Garjani The effects of 5HT3 receptor antagonist granisetron on inflammatory parameters and angiogenesis in the air-pouch model of inflammation Int. Immunopharmacol. 10 2010 1010 1016
61. A.J. Marcus, and L.B. Safier Thromboregulation: multicellular modulation of platelet reactivity in hemostasis and thrombosis FASEB J. 7 1993 516 522
62. S. Matsusaka, and I. Wakabayashi 5-Hydroxytryptamine as a potent migration enhancer of human aortic endothelial cells FEBS Lett. 579 2005 6721 6725
63. D.M. McDonald Angiogenesis and remodeling of airway vasculature in chronic inflammation Am. J. Respir. Crit. Care Med. 164 2001 S39 S45
64. W.G. Meijer, S.C. Copray, and H. Hollema Catecholamine-synthesizing enzymes in carcinoid tumors and pheochromocytomas Clin. Chem. 49 2003 586 593
65. E.J. Meredith, M.J. Holder, and A. Rosén Dopamine targets cycling B cells independent of receptors/transporter for oxidative attack: Implications for non-Hodgkin's lymphoma Proc. Natl. Acad. Sci. U. S. A. 103 2006 13485 13490
66. E. Mezey, G. Eisenhofer, and G. Harta A novel nonneuronal catecholaminergic system: exocrine pancreas synthesizes and releases dopamine Proc. Natl. Acad. Sci. U. S. A. 93 1996 10377 10382
67. L.F. Mohammad-Zadeh, L. Moses, and S.M. Gwaltney-Brant Serotonin: a review J. Vet. Pharmacol. Ther. 31 2008 187 199
68. M. Moreno-Smith, C. Lu, and M.M. Shahzad Dopamine blocks stress-mediated ovarian carcinoma growth Clin. Cancer Res. 17 2011 3649 3659
69. M. Moreno-Smith, S.J. Lee, and C. Lu Biologic effects of dopamine on tumor vasculature in ovarian carcinoma Neoplasia 15 2013 502 510
70. K. Müller, K.P. Gilbertz, and V. Meineke Serotonin and ionizing radiation synergistically affect proliferation and adhesion molecule expression of malignant melanoma cells J. Dermatol. Sci. 68 2012 89 98
71. G. Murphy, A.J. Cross, and L.S. Sansbury Dopamine D2 receptor polymorphisms and adenoma recurrence in the Polyp Prevention Trial Int. J. Cancer 124 2009 2148 2151
72. C.G. Nebigil, D.S. Choi, and A. Dierich Serotonin 2B receptor is required for heart development Proc. Natl. Acad. Sci. U. S. A. 97 2000 9508 9513
73. G.M. Nemecek, S.R. Coughlin, D.A. Handley, and M.A. Moskowitz Stimulation of aortic smooth muscle cell mitogenesis by serotonin Proc. Natl. Acad. Sci. U. S. A. 83 1986 674 678
74. W. Ni, and S.W. Watts 5-Hydroxytryptamine in the cardiovascular system: focus on the serotonin transporter (SERT) Clin. Exp. Pharmacol. Physiol. 33 2006 575 583
75. T. Nishikawa, N.H. Tsuno, and Y. Shuno Antiangiogenic effect of a selective 5-HT4 receptor agonist J. Surg. Res. 159 2010 696 704
76. A. Nocito, F. Dahm, and W. Jochum Serotonin regulates macrophage-mediated angiogenesis in a mouse model of colon cancer allografts Cancer Res. 68 2008 5152 5158
77. E. Novella-Maestre, C. Carda, and I. Noquera Dopamine agonist administration causes a reduction in endometrial implants through modulation of angiogenesis in experimentally induced endometriosis Hum. Reprod. 24 2009 1025 1035
78. E. Novella-Maestre, C. Carda, A. Ruiz-Sauri, J.A. Garcia-Velasco, C. Simon, and A. Pellicer Identification and quantification of dopamine receptor 2 in human eutopic and ectopic endometrium: a novel molecular target for endometriosis therapy Biol. Reprod. 83 2010 866 873
79. K.E. Ohlin, V. Francardo, and H.S. Lindgren Vascular endothelial growth factor is upregulated by l-DOPA in the parkinsonian brain: implications for development of dyskinesia Brain 134 2011 2339 2357
80. R. Pakala, and C.R. Benedict Effect of serotonin and thromboxane A2 on endothelial cell proliferation: effect of specific receptor antagonists J. Lab. Clin. Med. 131 1998 527 537
81. E. Papaleo, N. Doldi, and L. De Santis Cabergoline influences ovarian stimulation in hyperprolactinaemic patients with polycystic ovary syndrome Hum. Reprod. 16 2001 2263 2266
82. H.M. Pinedo, H.M. Verheul, R.J. D'Amato, and J. Folkman Involvement of platelets in tumour angiogenesis? Lancet 352 1998 1775 1777
83. I. Pirozhok, A. Meye, O.W. Hakenberg, S. Fuessel, and M.P. Wirth Serotonin and melatonin do not play a prominent role in the growth of prostate cancer cell lines Urol. Int. 84 2010 452 460
84. J. Profirovic, E. Strekalova, and N. Urao A novel regulator of angiogenesis in endothelial cells: 5-hydroxytriptamine 4 receptor Angiogenesis 16 2013 15 28
85. L. Qin, D. Zhao, and J. Xu The vascular permeabilizing factors histamine and serotonin induce angiogenesis through TR3/Nur77 and subsequently truncate it through thrombospondin-1 Blood 121 2013 2154 2164
86. W. Samborski, T. Stratz, S. Mackiewicz, and W. Müller Intra-articular treatment of arthritides and activated osteoarthritis with the 5-HT 3 receptor antagonist tropisestron. A double-blind study compared with methylprednisolone Scand. J. Rheumatol. Suppl. 119 2004 51 54
87. C. Sarkar, D. Chakroborty, R.B. Mitra, S. Banerjee, P.S. Dasgupta, and S. Basu Dopamine in vivo inhibits VEGF-induced phosphorylation of VEGFR-2, MAPK, and focal adhesion kinase in endothelial cells Am. J. Physiol. Heart Circ. Physiol. 287 2004 H1554 H1560
88. C. Sarkar, D. Chakroborty, U.R. Chowdhury, P.S. Dasgupta, and S. Basu Dopamine increases the efficacy of anticancer drugs in breast and colon cancer preclinical models Clin. Cancer Res. 14 2008 2502 2510
89. M. Schäfer, and S. Werner Cancer as an overhealing wound: an old hypothesis revisited Nat. Rev. Mol. Cell Biol. 9 2008 628 638
90. S.E. Senogles D2 dopamine receptor-mediated antiproliferation in a small cell lung cancer cell line, NCI-H69 Anticancer Drugs 18 2007 801 807
91. A. Shaltout, A. Shohyab, and M.A. Youssef Can dopamine agonist at a low dose reduce ovarian hyperstimulation syndrome in women at risk undergoing ICSI treatment cycles? A randomized controlled study Eur. J. Obstet. Gynecol. Reprod. Biol. 165 2012 254 258
92. S. Shome, T. Rana, and S. Ganguly Dopamine regulates angiogenesis in normal dermal wound tissues PloS One 6 2011 e25215
93. S. Shome, P.S. Dasgupta, and S. Basu Dopamine regulates mobilization of mesenchymal stem cells during wound angiogenesis PLoS One 7 2012 e31682
94. E.J. Siddiqui, M.A. Shabbir, D.P. Mikhailidis, F.H. Mumtaz, and C.S. Thompson The effect of serotonin and serotonin antagonists on bladder cancer cell proliferation BJU Int. 97 2006 634 639
95. 1B) receptors in prostate cancer cell proliferation J. Urol. 176 2006 1648 1653
96. A.J. Singer, and R.A. Clark Cutaneous wound healing N. Engl. J. Med. 341 1999 738 746
97. C. Soll, J.H. Jang, and M.O. Riener Serotonin promotes tumor growth in human hepatocellular cancer Hepatology 51 2010 1244 1254
98. C. Soll, M.O. Riener, and C.E. Oberkofler Expression of serotonin receptors in human hepatocellular cancer Clin. Cancer Res. 18 2012 5902 5910
99. B. Sonier, M. Arseneault, C. Lavigne, R.J. Ouellette, and C. Vaillancourt The 5-HT2A serotoninergic receptor is expressed in the MCF-7 human breast cancer cell line and reveals a mitogenic effect of serotonin Biochem. Biophys. Res. Commun. 343 2006 1053 1059
100. K. Tamura, T. Kanzaki, Y. Saito, M. Otabe, Y. Saito, and N. Morisaki Serotonin (5-hydroxytryptamine, 5-HT) enhances migration of rat aortic smooth muscle cells through 5-HT2 receptors Atherosclerosis 132 1997 139 143
101. M.A. Teunis, A. Kavelaars, and E. Voest Reduced tumor growth, experimental metastasis formation, and angiogenesis in rats with a hyperreactive dopaminergic system FASEB J. 16 2002 1465 1467
102. G.E. Torres, R.R. Gainetdinov, and M.G. Caron Plasma membrane monoamine transporters: structure, regulation and function Nat. Rev. Neurosci. 4 2003 13 25
103. L.M. Vicentini, M.G. Cattaneo, and R. Fesce Evidence for receptor subtype cross-talk in the mitogenic action of serotonin on human small-cell lung carcinoma cells Eur. J. Pharmacol. 318 1996 497 504
104. J.E. Westin, H.S. Lidgren, and J. Gardi Endothelial proliferation and increased blood-brain barrier permeability in the basal ganglia in a rat model of 3,4-dihydroxyphenyl-l-alanine-induced dyskinesia J. Neurosci. 26 2006 9448 9461
105. M.M. Wick The chemotherapy of malignant melanoma J. Invest. Dermatol. 80 1983 61s 62s
106. M. Yang, K. Li, and P.C. Ng Promoting effects of serotonin on hematopoiesis: ex vivo expansion of cord blood CD34+ stem/progenitor cells, proliferation on bone marrow stromal cells, and antiapoptosis Stem Cells 25 2007 1800 1806
107. M. Youssef, M. Van Wely, and M.A. Hassan Can dopamine agonists reduce the incidence and severity of OHSS in IVF/ICSI treatment cycles? A systematic review and meta-analysis Hum. Reprod. 16 2010 459 466
108. A. Zamani, and Z. Qu Serotonin activates angiogenic phosphorylation signaling in human endothelial cells FEBS Lett. 586 2012 2360 2365
109. D. Zhao, L. Qin, P.M. Bourbon, L. James, H.F. Dvorak, and H. Zeng Orphan nuclear transcription factor TR3/Nur77 regulates microvessel permeability by targeting endothelial nitric oxide synthase and destabilizing endothelial junctions Proc. Natl. Acad. Sci. U. S. A. 108 2011 12066 12071