Cucurbitacin B inhibits proliferation and induces apoptosis via STAT3 pathway inhibition in A549 lung cancer cells

Molecular Medicine Reports

Volumn 10, Issue 6, 2014, Pages 2905-2911

  Zhang, Meng ^a   Bian, Zhi Gang ^a   Zhang, Yi ^a   Wang, Jia He ^a   Kan, Liang ^a   Wang, Xin ^a   Niu, Hui Yan ^a   He, Ping ^a  

^a CHINA MEDICAL UNIVERSITY   (China)

Author keywords

A549 lung cancer cell line; Apoptosis; Cucurbitacin B; STAT3

Indexed keywords

ANTINEOPLASTIC AGENT; CUCURBITACIN B; CYCLIN B1; CYTOCHROME C; PROTEIN BCL 2; STAT3 PROTEIN; STAT3 PROTEIN, HUMAN; TRITERPENE;

APOPTOSIS; CELL CYCLE; CELL PROLIFERATION; DOWN REGULATION; DRUG EFFECTS; HUMAN; LUNG NEOPLASMS; METABOLISM; SIGNAL TRANSDUCTION; TUMOR CELL LINE;

ANTINEOPLASTIC AGENTS; APOPTOSIS; CELL CYCLE; CELL LINE, TUMOR; CELL PROLIFERATION; CYCLIN B1; CYTOCHROMES C; DOWN-REGULATION; HUMANS; LUNG NEOPLASMS; PROTO-ONCOGENE PROTEINS C-BCL-2; SIGNAL TRANSDUCTION; STAT3 TRANSCRIPTION FACTOR; TRITERPENES;

EID: 84914172158     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2014.2581     Document Type: Article

References (27)

1. Jemal A, Bray F, Center MM, et al: Global cancer statistics. CA Cancer J Clin 61: 69-90, 2011.
2. Schiller JH, Harrington D, Belani CP, et al: Comparison of four chemotherapy regimens for advanced non-small-cell lung cancer. N Engl J Med 346: 92-98, 2002.
3. Zhang Y, Zhang GB, Xu XM, et al: Suppression of growth of A549 lung cancer cells by waltonitone and its mechanisms of action. Oncol Rep 28: 1029-1035, 2012.
4. Reyes-Zurita FJ, Rufino-Palomares EE, Lupiañez JA and Cascante M: Maslinic acid, a natural triterpene from Olea europaea L., induces apoptosis in HT29 human colon-cancer cells via the mitochondrial apoptotic pathway. Cancer Lett 273: 44-54, 2009.
5. Liu T, Zhang M, Zhang H, et al: Combined antitumor activity of cucurbitacin B and docetaxel in laryngeal cancer. Eur J Pharmacol 587: 78-84, 2008.
6. Jayaprakasam B, Seeram NP and Nair MG: Anticancer and antiinflammatory activities of cucurbitacins from Cucurbita andreana. Cancer Lett 189: 11-16, 2003.
7. Kongtun S, Jiratchariyakul W, Kummalue T, et al: Cytotoxic properties of root extract and fruit juice of Trichosanthes cucumerina. Planta Med 75: 839-842, 2009.
8. Zhang M, Zhang H, Sun C, et al: Targeted constitutive activation of signal transducer and activator of transcription 3 in human hepatocellular carcinoma cells by cucurbitacin B. Cancer Chemother Pharmacol 63: 635-642, 2009.
9. Hsu HF, Houng JY, Kuo CF, Tsao N and Wu YC: Glossogin, a novel phenylpropanoid from Glossogyne tenuifolia, induced apoptosis in A549 lung cancer cells. Food Chem Toxicol 46: 3785-3791, 2008.
10. Magesh V, Lee JC, Ahn KS, et al: Ocimum sanctum induces apoptosis in A549 lung cancer cells and suppresses the in vivo growth of Lewis lung carcinoma cells. Phytother Res 23: 1385-1391, 2009.
11. Thoennissen NH, Iwanski GB, Doan NB, et al: Cucurbitacin B induces apoptosis by inhibition of the JAK/STAT pathway and potentiates antiproliferative effects of gemcitabine on pancreatic cancer cells. Cancer Res 69: 5876-5884, 2009.
12. Liu T, Zhang M, Zhang H, Sun C and Deng Y: Inhibitory effects of cucurbitacin B on laryngeal squamous cell carcinoma. Eur Arch Otorhinolaryngol 265: 1225-1232, 2008.
13. Chan KT, Meng FY, Li Q, et al: Cucurbitacin B induces apoptosis and S phase cell cycle arrest in BEL-7402 human hepatocellular carcinoma cells and is effective via oral administration. Cancer Lett 294: 118-124, 2010.
14. Xu X, Zhang Y, Qu D, Jiang T and Li S: Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway. J Exp Clin Cancer Res 30: 33, 2011.
15. Jackman M, Lindon C, Nigg EA and Pines J: Active cyclin B1-Cdk1 first appears on centrosomes in prophase. Nat Cell Biol 5: 143-148, 2003.
16. Yasuda S, Yogosawa S, Izutani Y, et al: Cucurbitacin B induces G2 arrest and apoptosis via a reactive oxygen species-dependent mechanism in human colon adenocarcinoma SW480 cells. Mol Nutr Food Res 54: 559-565, 2010.
17. Yang L, Wu S, Zhang Q, Liu F and Wu P: 23,24-Dihydrocucurbitacin B induces G2/M cell-cycle arrest and mitochondria-dependent apoptosis in human breast cancer cells (Bcap37). Cancer Lett 256: 267-278, 2007.
18. Braun F, de Carne Trecesson S, Bertin-Ciftci J and Juin P: Protect and serve: Bcl-2 proteins as guardians and rulers of cancer cell survival. Cell Cycle 12: 2937-2947, 2013.
19. Yang J, Liu X, Bhalla K, et al: Prevention of apoptosis by Bcl-2: Release of cytochrome C from mitochondria blocked. Science 275: 1129-1132, 1997.
20. Hu Y, Benedict MA, Ding L and Nuñez G: Role of cytochrome C and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis. EMBO J 18: 3586-3595, 1999.
21. Malladi S, Challa-Malladi M, Fearnhead HO and Bratton SB: The Apaf-1 procaspase-9 apoptosome complex functions as a proteolytic-based molecular timer. EMBO J 28: 1916-1925, 2009.
22. Inoue S, Browne G, Melino G and Cohen GM: Ordering of caspases in cells undergoing apoptosis by the intrinsic pathway. Cell Death Differ 16: 1053-1061, 2009.
23. Niu G, Wright KL, Huang M, et al: Constitutive Stat3 activity up-regulates VEGF expression and tumor angiogenesis. Oncogene 21: 2000-2008, 2002.
24. Diaz N, Minton S, Cox C, et al: Activation of stat3 in primary tumors from high-risk breast cancer patients is associated with elevated levels of activated SRC and survivin expression. Clin Cancer Res 12: 20-28, 2006.
25. Yeh HH, Chang WT, Lu KC, et al: Upregulation of tissue factor by activated Stat3 contributes to malignant pleural effusion generation via enhancing tumor metastasis and vascular permeability in lung adenocarcinoma. PLoS One 8: E75287, 2013.
26. Chan KS, Sano S, Kiguchi K, et al: Disruption of Stat3 reveals a critical role in both the initiation and the promotion stages of epithelial carcinogenesis. J Clin Invest 114: 720-728, 2004.
27. Yu H and Jove R: The STATs of cancer - new molecular targets come of age. Nat Rev Cancer 4: 97-105, 2004.