Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

Journal of Assisted Reproduction and Genetics

Volumn 31, Issue 12, 2014, Pages 1673-1680

  Petyim, Somsin ^a   Neungton, Chanon ^a   Thanaboonyawat, Isarin ^a   Laokirkkiat, Pitak ^a   Choavaratana, Roungsin ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Apoptosis; Cryopreservation; Motility; Swim up; Vitality

Indexed keywords

ADULT; APOPTOSIS; ARTICLE; CONTROLLED STUDY; CRYOPRESERVATION; FREEZE THAWING; HUMAN; MAJOR CLINICAL STUDY; MALE; MALE INFERTILITY; SEMEN ANALYSIS; SPERM; SPERMATOZOON; SPERMATOZOON COUNT; SPERMATOZOON MOTILITY; DNA DAMAGE; FLOW CYTOMETRY; GENETICS; PATHOLOGY; PHYSIOLOGY; SPERM PRESERVATION;

ADULT; APOPTOSIS; CRYOPRESERVATION; DNA DAMAGE; FLOW CYTOMETRY; HUMANS; INFERTILITY, MALE; MALE; SEMEN ANALYSIS; SEMEN PRESERVATION; SPERM COUNT; SPERM MOTILITY;

EID: 84914159575     PISSN: 10580468     EISSN: 15737330     Source Type: Journal    
DOI: 10.1007/s10815-014-0332-y     Document Type: Article

References (38)

1. Nangia AK, Krieg SA, Kim SS. Clinical guidelines for sperm cryopreservation in cancer patients. Fertil Steril. 2013;100:1203–9.
2. Petyim S, Choavaratana R. Cryodamage on sperm chromatin according to different freezing methods, assessed by AO test. J Med Assoc Thai. 2006;89:306–13.
3. Critser JK, Arneson BW, Aaker DV, Huse-Benda AR, Ball GD. Cryopreservation of human spermatozoa. II. Postthaw chronology of motility and of zona-free hamster ova penetration. Fertil Steril. 1987;47:980–4.
4. Hammadeh ME, Askari AS, Georg T, Rosenbaum P, Schmidt W. Effect of freeze-thawing procedure on chromatin stability, morphological alteration and membrane integrity of human spermatozoa in fertile and subfertile men. Int J Androl. 1999;22:155–62.
5. Royere D, Hamamah S, Nicolle JC, Lansac J. Chromatin alterations induced by freeze-thawing influence the fertilizing ability of human sperm. Int J Androl. 1991;14:328–32.
6. Thomson LK, Fleming SD, Aitken RJ, De Iuliis GN, Zieschang JA, Clark AM. Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Hum Reprod. 2009;24:2061–70.
7. Abush A, Hauser R, Paz G, Kleiman SE, Lehavi O, Yavetz H, et al. Thawed human sperm quality is influenced by the volume of the cryopreserved specimen. Fertil Steril. 2014;101:640–6.
8. Ribas-Maynou J, Fernandez-Encinas A, Garcia-Peiro A, Prada E, Abad C, Amengual MJ, et al. Human semen cryopreservation: a sperm DNA fragmentation study with alkaline and neutral Comet assay. Andrology. 2014;2:83–7.
9. Larson KL, DeJonge CJ, Barnes AM, Jost LK, Evenson DP. Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques. Hum Reprod. 2000;15:1717–22.
10. Hoshi K, Katayose H, Yanagida K, Kimura Y, Sato A. The relationship between acridine orange fluorescence of sperm nuclei and the fertilizing ability of human sperm. Fertil Steril. 1996;66:634–9.
11. Twigg J, Fulton N, Gomez E, Irvine DS, Aitken RJ. Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants. Hum Reprod. 1998;13:1429–36.
12. Ricci G, Perticarari S, Boscolo R, Simeone R, Martinelli M, Fischer-Tamaro L, et al. Leukocytospermia and sperm preparation–a flow cytometric study. Reprod Biol Endocrinol. 2009;7:128.
13. Demir B, Arikan II, Bozdag G, Esinler I, Karakoc Sokmensuer L, Gunalp S. Effect of sperm morphology on clinical outcome parameters in ICSI cycles. Clin Exp Obstet Gynecol. 2012;39:144–6.
14. Whittington K, Ford WC. Relative contribution of leukocytes and of spermatozoa to reactive oxygen species production in human sperm suspensions. Int J Androl. 1999;22:229–35.
15. Aitken RJ, Buckingham D, West K, Wu FC, Zikopoulos K, Richardson DW. Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygen species in the ejaculates of oligozoospermic patients and fertile donors. J Reprod Fertil. 1992;94:451–62.
16. Henkel R, Kierspel E, Stalf T, Mehnert C, Menkveld R, Tinneberg HR, et al. Effect of reactive oxygen species produced by spermatozoa and leukocytes on sperm functions in non-leukocytospermic patients. Fertil Steril. 2005;83:635–42.
17. Said TM, Agarwal A, Sharma RK, Mascha E, Sikka SC, Thomas Jr AJ. Human sperm superoxide anion generation and correlation with semen quality in patients with male infertility. Fertil Steril. 2004;82:871–7.
18. Brugnon F, Ouchchane L, Pons-Rejraji H, Artonne C, Farigoule M, Janny L. Density gradient centrifugation prior to cryopreservation and hypotaurine supplementation improve post-thaw quality of sperm from infertile men with oligoasthenoteratozoospermia. Hum Reprod. 2013;28:2045–57.
19. Valcarce DG, Carton-Garcia F, Riesco MF, Herraez MP, Robles V. Analysis of DNA damage after human sperm cryopreservation in genes crucial for fertilization and early embryo development. Andrology. 2013;1:723–30.
20. Rougier N, Uriondo H, Papier S, Checa MA, Sueldo C, Alvarez SC. Changes in DNA fragmentation during sperm preparation for intracytoplasmic sperm injection over time. Fertil Steril. 2013;100:69–74.
21. Justice T, Christensen G. Sperm cryopreservation methods. Methods Mol Biol. 2013;927:209–15.
22. Sharma RK, Said T, Agarwal A. Sperm DNA damage and its clinical relevance in assessing reproductive outcome. Asian J Androl. 2004;6:139–48.
23. Esteves SC, Sharma RK, Thomas Jr AJ, Agarwal A. Effect of swim-up sperm washing and subsequent capacitation on acrosome status and functional membrane integrity of normal sperm. Int J Fertil Womens Med. 2000;45:335–41.
24. Thomson LK, Fleming SD, Barone K, Zieschang JA, Clark AM. The effect of repeated freezing and thawing on human sperm DNA fragmentation. Fertil Steril. 2010;93:1147–56.
25. Branco CS, Garcez ME, Pasqualotto FF, Erdtman B, Salvador M. Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen. Cryobiology. 2009;60:235–7.
26. Li Z, Lin Q, Liu R, Xiao W, Liu W. Protective Effects of Ascorbate and Catalase on Human Spermatozoa During Cryopreservation. J Androl. 2009;31:437–44.
27. WHO. Cambridge; Cambridge University Press; 2010. WHO laboratory manual for the examination and processing of human semen.
28. Esteves SC, Sharma RK, Thomas Jr AJ, Agarwal A. Improvement in motion characteristics and acrosome status in cryopreserved human spermatozoa by swim-up processing before freezing. Hum Reprod. 2000;15:2173–9.
29. Counsel M, Bellinge R, Burton P. Vitality of oligozoospermic semen samples is improved by both swim-up and density gradient centrifugation before cryopreservation. J Assist Reprod Genet. 2004;21:137–42.
30. Abeysundara PK, Dissanayake D, Wijesinghe PS, Perera R, Nishad A. Efficacy of two sperm preparation techniques in reducing non-specific bacterial species from human semen. J Hum Reprod Sci. 2013;6:152–7.
31. Stanic P, Tandara M, Sonicki Z, Simunic V, Radakovic B, Suchanek E. Comparison of protective media and freezing techniques for cryopreservation of human semen. Eur J Obstet Gynecol Reprod Biol. 2000;91:65–70.
32. Sion B, Janny L, Boucher D, Grizard G. Annexin V binding to plasma membrane predicts the quality of human cryopreserved spermatozoa. Int J Androl. 2004;27:108–14.
33. Rodriguez-Martinez H, Larsson B, Pertoft H. Evaluation of sperm damage and techniques for sperm clean-up. Reprod Fertil Dev. 1997;9:297–308.
34. Muratori M, Porazzi I, Luconi M, Marchiani S, Forti G, Baldi E. AnnexinV binding and merocyanine staining fail to detect human sperm capacitation. J Androl. 2004;25:797–810.
35. Tavalaee M, Deemeh MR, Arbabian M, Nasr-Esfahani MH. Density gradient centrifugation before or after magnetic-activated cell sorting: which technique is more useful for clinical sperm selection? J Assist Reprod Genet. 2012;29:31–8.
36. Grunewald S, Paasch U. Sperm selection for ICSI using annexin V. Methods Mol Biol. 2013;927:257–62.
37. Martin G, Sabido O, Durand P, Levy R. Phosphatidylserine externalization in human sperm induced by calcium ionophore A23187: relationship with apoptosis, membrane scrambling and the acrosome reaction. Hum Reprod. 2005;20:3459–68.
38. Ricci G, Boscolo R, Martinelli M, Perticarari S. Methods for defecting sperm apoptosis. Fertil Steril. 2009;92(2):e19.