Methods for defecting sperm apoptosis

Fertility and Sterility

Volumn 92, Issue 2, 2009, Pages

  Ricci, Giuseppe ^a   Boscolo, Rita ^a   Martinelli, Monica ^a   Perticarari, Sandra ^a  

^a Institute for Maternal and Child Health   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

APOPTOSIS; CELL DEATH; HUMAN; LETTER; PRIORITY JOURNAL; SAMPLE SIZE; SEMEN ABNORMALITY; SEMEN ANALYSIS; SPERM; SPERMATOZOON MOTILITY;

APOPTOSIS; CHROMATIN; DNA FRAGMENTATION; HUMANS; MALE; PLOIDIES; SEMEN ANALYSIS; SPERM COUNT; SPERMATOZOA; TOLONIUM CHLORIDE;

EID: 67651095768     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fertnstert.2008.12.109     Document Type: Letter

References (7)

1. Mahfouz RZ, Sharma RK, Said TM, Erenpreiss J, Agarwal A. Association of sperm apoptosis and DNA ploidy with sperm chromatin quality in human spermatozoa. Fertil Steril. Published online May 5, 2008 [Epub ahead of print].
2. Paasch U., Grunewald S., Fitzl G., and Glander H.J. Deterioration of plasma membrane is associated with activated caspases in human spermatozoa. J Androl 24 (2003) 246-252
3. Marchetti C., Gallego M.A., Deffosez A., Formstecher P., and Marchetti P. Staining of human sperm with fluorochrome-labeled inhibitor of caspases to detect activated caspases: correlation with apoptosis and sperm parameters. Hum Reprod 19 (2004) 1127-1134
4. Ricci G, Perticarari S, Boscolo R, Montico M, Guaschino S, Presani G. Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique. Fertil Steril. Published online January 16, 2008 [Epub ahead of print].
5. Ricci G., Perticarari S., Fragonas E., Giolo E., Canova S., Pozzobon C., et al. Apoptosis in human sperm: its correlation with semen quality and the presence of leukocytes. Hum Reprod 17 (2002) 2665-2672
6. Koopman G., Reutelingsperger C.P., Kuijten G.A., Keehnen R.M., Pals S.T., and van Oers M.H. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84 (1994) 1415-1420
7. Perticarari S., Ricci G., Granzotto M., Boscolo R., Pozzobon C., Guarnieri S., et al. A new multiparameter flow cytometric method for human semen analysis. Hum Reprod 22 (2007) 485-494