Simultaneous production of butanol and acetoin by metabolically engineered Clostridium acetobutylicum

Metabolic Engineering

Volumn 27, Issue , 2015, Pages 107-114

  Liu, Dong ^a   Chen, Yong ^a   Ding, Fengying ^a   Guo, Ting ^a   Xie, Jingjing ^a   Zhuang, Wei ^a   Niu, Haunqing ^a   Shi, Xinchi ^a   Zhu, Chenjie ^a   Ying, Hanjie ^a  

^a NANJING TECH UNIVERSITY   (China)

Author keywords

Acetoin; Acetolactate decarboxylase; AlsR; Clostridium acetobutylicum; HydA; Redox balance

Indexed keywords

CLOSTRIDIUM; FERMENTATION; GENES; METABOLIC ENGINEERING;

ACETOIN; ALSR; CLOSTRIDIUM ACETOBUTYLICUM; DECARBOXYLASES; HYDA; REDOX BALANCES;

ACETONE;

2 BUTANOL; 2,3 BUTANEDIOL; ACETOIN; ACETONE; ALCOHOL; ALPHA ACETOLACTATE DECARBOXYLASE; BUTANOL; CARBOXYLYASE; UNCLASSIFIED DRUG; ACETOLACTATE DECARBOXYLASE; BACTERIAL PROTEINS; BUTANOLS; CARBOXY-LYASES;

ALCOHOL PRODUCTION; ARTICLE; BINDING SITE; CLOSTRIDIUM ACETOBUTYLICUM; DECARBOXYLATION; EXTRACTIVE FERMENTATION; GENE OVEREXPRESSION; METABOLIC ENGINEERING; NONHUMAN; TRANSCRIPTION REGULATION; BIOSYNTHESIS; ENZYMOLOGY; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENETICS; METABOLISM; MUTATION;

CLOSTRIDIUM ACETOBUTYLICUM;

ACETOIN; BACTERIAL PROTEINS; BUTANOLS; CARBOXY-LYASES; CLOSTRIDIUM ACETOBUTYLICUM; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; MUTATION;

EID: 84914149151     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2014.11.002     Document Type: Article

References (39)

1. Baer S.H., Blaschek H.P., Smith T.L. Effect of butanol challenge and temperature on lipid composition and membrane fluidity of butanol-tolerant Clostridium acetobutylicum. Appl. Environ. Microbiol. 1987, 53:2854-2861.
2. Barak Z., Chipman D.M. Allosteric regulation in Acetohydroxyacid Synthases (AHASs) - different structures and kinetic behavior in isozymes in the same organisms. Arch. Biochem. Biophys. 2012, 519:167-174.
3. Cai X.P., Bennett G.N. Improving the Clostridium acetobutylicum butanol fermentation by engineering the strain for co-production of riboflavin. J. Ind. Microbiol. Biotechnol. 2011, 38:1013-1025.
4. Collas F., Kuit W., Clément B., Marchal R., López-Contreras A.M., Monot F. Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains. AMB Express 2012, 2:45.
5. Cooksley C.M., Zhang Y., Wang H., Redl S., Winzer K., Minton N.P. Targeted mutagenesis of the Clostridium acetobutylicum acetone-butanol-ethanol fermentation pathway. Metab. Eng. 2012, 14:630-641.
6. Dai Z.J., Dong H.J., Zhu Y., Zhang Y.P., Li Y., Ma Y.H. Introducing a single secondary alcohol dehydrogenase into butanol-tolerant Clostridium acetobutylicum Rh8 switches ABE fermentation to high level IBE fermentation. Biotechnol. Biofuels 2012, 5:44.
7. Desai R.P., Harris L.M., Welker N.E., Papoutsakis E.T. Metabolic flux analysis elucidates the importance of the acid-formation pathways in regulating solvent production by Clostridium acetobutylicum. Metab. Eng. 1999, 1:206-213.
8. Doremus M.G., Linden J.C., Moreira A.R. Agitation and pressure effects on acetone-butanol fermentation. Biotechnol. Bioeng. 1984, 27:852-860.
9. Fradrich C., March A., Fiege K., Hartmann A., Jahn D., Hartig E. The transcription factor AlsR binds and regulates the promoter of the alsSD operon responsible for acetoin formation in Bacillus subtilis. J. Bacteriol. 2012, 194:1100-1112.
10. Green E.M. Fermentative production of butanol-the industrial perspective. Curr. Opin. Biotechnol. 2011, 22:1-7.
11. Gu Y., Jiang Y., Wu H., Liu X., Li Z., Li J., Xiao H., Shen Z., Dong H., Yang Y., Li Y., Jiang W., Yang S. Economical challenges to microbial producers of butanol: feedstock, butanol ratio and titer. Biotechnol. J. 2011, 6:1348-1357.
12. Hartmanis M.G.N., Klason T., Gatenbeck S. Uptake and activation of acetate and butyrate in Clostridium acetobutylicum. Appl. Microbiol. Biotechnol. 1984, 20:66-71.
13. Heap J.T., Kuehne S.A., Ehsaan M., Cartman S.T., Cooksley C.M., Scott J.C., Minton N.P. The ClosTron: mutagenesis in Clostridium refined and streamlined. J. Microbiol. Methods 2010, 80:49-55.
14. Heap J.T., Pennington O.J., Cartman S.T., Carter G.P., Minton N.P. The ClosTron: a universal gene knock-out system for the genus Clostridium. J. Microbiol. Methods 2007, 70:452-464.
15. Jiang Y., Xu C., Dong F., Yang Y., Jiang W., Yang S. Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metab. Eng. 2009, 11:284-291.
16. Jones D.T., Woods D.R. Acetone-butanol fermentation revisited. Microbiol. Rev. 1986, 50:484-524.
17. Kovacikova G., Lin W., Skorupski K. Dual regulation of genes involved in acetoin biosynthesis and motility/biofilm formation by the virulence activator AphA and the acetate-responsive LysR-type regulator AlsR in Vibrio cholerae. Mol. Microbiol. 2005, 57:420-433.
18. Lee J., Jang Y.S., Choi S.J., Im J.A., Song H., Cho J.H., Seung do Y., Papoutsakis E.T., Bennett G.N., Lee S.Y. Metabolic engineering of Clostridium acetobutylicum ATCC 824 for isopropanol-butanol-ethanol fermentation. Appl. Environ. Microbiol. 2011, 78:1416-1423.
19. Lehmann D., Hönicke D., Ehrenreich A., Schmidt M., Weuster-Botz D., Bahl H., Lütke-Eversloh T. Modifying the product pattern of Clostridium acetobutylicum. Appl. Microbiol. Biotechnol. 2012, 94:743-754.
20. Li S., Xu N., Liu L., Chen J. Engineering of carboligase activity reaction in Candida glabrata for acetoin production. Metab. Eng. 2014, 22:32-39.
21. Liu D., Chen Y., Ding F.Y., Zhao T., Wu J.L., Guo T., Ren H.F., Li B.B., Niu H.Q., Cao Z., Lin X.Q., Xie J.J., He X.J., Ying H.J. Biobutanol production in a Clostridium acetobutylicum biofilm reactor integrated with simultaneous product recovery by adsorption. Biotechnol. Biofuels 2014, 7:5.
22. Liu D., Chen Y., Li A., Ding F.Y., Zhou T., He Y., Li B.B., Niu H.Q., Lin X.Q., Xie J.J, Chen X.C., Wu J.L., Ying H.J. Enhanced butanol production by modulation of electron flow in Clostridium acetobutylicum B3 immobilized by surface adsorption. Bioresour. Technol. 2013, 129:321-328.
23. Maddocks S.E., Oyston P.C. Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins. Microbiology 2008, 154:3609-3623.
24. Mayer D., Schlensog V., Bock A. Identification of the transcriptional activator controlling the butanediol fermentation pathway in Klebsiella terrigena. J. Bacteriol. 1995, 177:5261-5269.
25. Mermelstein L.D., Papoutsakis E.T. In vivo methylation in Escherichia coli by the Bacillus subtilis phage phi 3 T I methyltransferase to protect plasmids from restriction upon transformation of Clostridium acetobutylicum ATCC824. Appl. Environ. Microbiol. 1993, 59:1077-1081.
26. Mermelstein L.D., Welker N.E., Bennett G.N., Papoutsakis E.T. Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824. Biotechnology 1992, 10:190-195.
27. Moons P., Van Houdt R., Vivijs B., Michiels C.M., Aertsen A. Integrated Regulation of Acetoin Fermentation by Quorum Sensing and pH in Serratia plymuthica RVH1. Appl. Environ. Microbiol. 2011, 77:3422-3427.
28. Nolling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J. Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. J. Bacteriol. 2001, 183:4823-4838.
29. Peguin S., Goma G., Delorme P., Soucaille P. Metabolic flexibility of Clostridium acetobutylicum in response to methyl viologen. Appl. Microbiol. Biotechnol. 1994, 42:611-616.
30. Ren C., Gu Y., Hu S., Wu Y., Wang P., Yang Y., Yang C., Yang S., Jiang W. Identification and inactivation of pleiotropic regulator CcpA to eliminate glucose repression of xylose utilization in Clostridium acetobutylicum. Metab. Eng. 2010, 12:446-454.
31. Renna M.C., Najimudin N., Winik L.R., Zahler S.A. Regulation of the Bacillus subtilis alsS, alsD, and alsR genes involved in post-exponential-phase production of acetoin. J. Bacteriol. 1993, 175:3863-3875.
32. Savakis P.E., Angermayr S.A., Hellingwerf K.J. Synthesis of 2, 3-butanediol by Synechocystis sp. PCC6803 via heterologous expression of a catabolic pathway from lactic acid- and enterobacteria. Metab. Eng. 2013, 20:121-130.
33. Siemerink M.A., Kuit W., López Contreras A.M., Eggink G., van der Oost J., Kengen S.W. D-2,3-Butanediol production due to heterologous expression of an acetoin reductase in Clostridium acetobutylicum. Appl. Environ. Microbiol. 2011, 77:2582-2588.
34. Tracy B.P. Improving butanol fermentation to enter the advanced biofuel market. mBio 2012, 3:e00518-12.
35. Wardwell S. Metabolism of Acetoin in Clostridium acetobutylicum ATCC 824 1999, (Ph.D. thesis), Rice University, Houston.
36. Xiao Z., Lv C., Gao C., Qin J., Ma C., Liu Z., Liu P., Li L., Xu P. A novel whole-cell biocatalyst with NAD+ regeneration for production of chiral chemicals. PLoS One 2010, 5:e8860.
37. Xiao Z.J., Xu P. Acetoin Metabolism in Bacteria. Crit. Rev. Microbiol. 2007, 33:127-140.
38. Xu Y., Chu H., Gao C., Tao F., Zhou Z., Li K., Li L., Ma C., Xu P. Systematic metabolic engineering of Escherichia coli for high-yield production of fuel bio-chemical 2,3-butanediol. Metab. Eng. 2014, 23:22-33.
39. Zhang X., Zhang R., Bao T., Rao Z., Yang T., Xu M., Xu Z., Li H., Yang S. The rebalanced pathway significantly enhances acetoin production by disruption of acetoin reductase gene and moderate-expression of a new water-forming NADH oxidase in Bacillus subtilis. Metab. Eng. 2014, 23:34-41.