Dental pulp stem cells' secretome enhances pulp repair processes and compensates TEGDMA-induced cytotoxicity

Dental Materials

Volumn 30, Issue 12, 2014, Pages e405-e418

  Paschalidis, Theodoros ^a   Bakopoulou, Athina ^a   Papa, Polyxeni ^a   Leyhausen, Gabriele ^b   Geurtsen, Werner ^b   Koidis, Petros ^a  

^a ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

^b HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Autocrine paracrine signaling; Conditioned medium (CM); Dental pulp stem cells (DPSCs); Pulp repair; TEGDMA

Indexed keywords

CELL SIGNALING; CYTOLOGY; CYTOTOXICITY; MINERALOGY;

AUTOCRINE SIGNALING; CONCENTRATION-DEPENDENT; CONDITIONED MEDIUM; DENTAL PULP STEM CELLS; MINERALIZATION POTENTIAL; PRESENCE/ABSENCE; SERUM DEPRIVATION; TEGDMA;

STEM CELLS;

ALIZARIN RED S; ANTHRAQUINONE DERIVATIVE; COLORING AGENT; CONDITIONED MEDIUM; DENTAL MATERIAL; FIBROBLAST GROWTH FACTOR 2; MACROGOL DERIVATIVE; POLYMETHACRYLIC ACID DERIVATIVE; TETRAZOLIUM; THIAZOLE DERIVATIVE; THIAZOLYL BLUE; TRANSFORMING GROWTH FACTOR BETA1; TRIETHYLENE GLYCOL DIMETHACRYLATE;

ADOLESCENT; AUTOCRINE EFFECT; BONE MINERALIZATION; CELL CULTURE; CELL CULTURE TECHNIQUE; CELL MOTION; CELL SURVIVAL; COMPARATIVE STUDY; CONDITIONED MEDIUM; CYTOLOGY; DRUG EFFECTS; HUMAN; PHYSIOLOGY; SECRETION (PROCESS); STEM CELL; TOOTH PULP;

ADOLESCENT; ANTHRAQUINONES; AUTOCRINE COMMUNICATION; CALCIFICATION, PHYSIOLOGIC; CELL CULTURE TECHNIQUES; CELL MOVEMENT; CELL SURVIVAL; CELLS, CULTURED; COLORING AGENTS; CULTURE MEDIA, CONDITIONED; DENTAL MATERIALS; DENTAL PULP; FIBROBLAST GROWTH FACTOR 2; HUMANS; POLYETHYLENE GLYCOLS; POLYMETHACRYLIC ACIDS; STEM CELLS; TETRAZOLIUM SALTS; THIAZOLES; TRANSFORMING GROWTH FACTOR BETA1;

EID: 84912105672     PISSN: 01095641     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.dental.2014.08.377     Document Type: Article

References (50)

1. Murray PE, Windsor LJ, Smyth TW, Hafez AA, Cox CF. Analysis of pulpal reactions to restorative procedures, materials, pulp capping and future therapies. Crit Rev Oral Biol Med 2002;13:509-20.
2. Goldberg M, Farges JC, Lacerda-Pinheiro S, Six N, Jegat N, Decup F, et al. Inflammatory and immunological aspects of dental pulp repair. Pharmacol Res 2008;58(2):137-47.
3. About I. Dentin-pulp regeneration: the primordial role of the microenvironment and its modification by traumatic injuries and bioactive materials. Endod Top 2013;28:61-89.
4. Huang GTJ, Gronthos S, Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res 2009;88(9):792-806.
5. Shi S, Gronthos S. Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J Bone Miner Res 2003;18:696-704.
6. Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A, et al. Stem cell properties of human dental pulp stem cells. J Dent Res 2002;81(8):531-5.
7. Batouli S, Miura M, Brahim J, Tsutsui TW, Fisher LW, Gronthos S, et al. Comparison of stem-cell-mediated osteogenesis and dentinogenesis. J Dent Res 2003;82(12):976-81.
8. Gnecchi M, Zhang Z, Ni A, Dzau VJ. Paracrine mechanisms in adult stem cell signaling and therapy. Circ Res 2008;103(11):1204-19.
9. Meirelles Lda S, Fontes AM, Covas DT, Caplan AI. Mechanisms involved in the therapeutic properties of mesenchymal stem cells. Cytokine Growth Factor Rev 2009;20(5-6):419-27.
10. Hwang NS, Zhang C, Hwang YS, Varghese S. Mesenchymal stem cell differentiation and roles in regenerative medicine. Wiley Interdiscip Rev Syst Biol Med 2009;1(1):97-106.
11. Chen L, Tredget EE, Wu PY, Wu Y. Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and enhance wound healing. PLoS ONE 2008;3(4):e1886.
12. Lee SH, Jin SY, Song JS, Seo KK, Cho KH. Paracrine effects of adipose-derived stem cells on keratinocytes and dermal fibroblasts. Ann Dermatol 2012;24(2):136-43.
13. Geurtsen W. Biocompatibility of resin-modified filling materials. Crit Rev Oral Biol Med 2000;11(3):333-55.
14. de Souza Costa CA, Teixeira HM, Lopes do Nascimento AB, Hebling J. Biocompatibility of resin-based dental materials applied as liners in deep cavities prepared in human teeth. J Biomed Mater Res B Appl Biomater 2007;81(1):175-84.
15. Accorinte ML, Loguercio AD, Reis A, Costa CA. Response of human pulps capped with different self-etch adhesive systems. Clin Oral Invest 2008;12(2):119-27.
16. Camps J, Déjou J, Rémusat M, About I. Factors influencing pulpal response to cavity restorations. Dent Mater 2000;16(6):432-40.
17. About I, Camps J, Mitsiadis TA, Bottero MJ, Butler W, Franquin JC. Influence of resinous monomers on the differentiation in vitro of human pulp cells into odontoblasts. J Biomed Mater Res B Appl Biomater 2002;63:418-23.
18. Bakopoulou A, Leyhausen G, Volk J, Tsiftsoglou A, Garefis P, Koidis P, et al. Effects of HEMA and TEGDMA on the in vitro odontogenic differentiation potential of human pulp stem/progenitor cells derived from deciduous teeth. Dent Mater 2011;27(6):608-17.
19. Galler KM, Schweikl H, Hiller KA, Cavender AC, Bolay C, D'Souza RN, et al. TEGDMA reduces mineralization in dental pulp cells. J Dent Res 2011;90(2):257-62.
20. Krifka S, Spagnuolo G, Schmalz G, Schweikl H. A review of adaptive mechanisms in cell responses towards oxidative stress caused by dental resin monomers. Biomaterials 2013;34(19):4555-63.
21. Eckhardt A, Gerstmayr N, Hiller KA, Bolay C, Waha C, Spagnuolo G, et al. TEGDMA-induced oxidative DNA damage and activation of ATM and MAP kinases. Biomaterials 2009;30(11):2006-14.
22. Liang CC, Park AY, Guan JL. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2007;2(2):329-33.
23. Baraniak PR, McDevitt TC. Stem cell paracrine actions and tissue regeneration. Regen Med 2010;5(1):121-43.
24. Oskowitz A, McFerrin H, Gutschow M, Carter ML, Pochampally R. Serum-deprived human multipotent mesenchymal stromal cells (MSCs) are highly angiogenic. Stem Cell Res 2011;6(3):215-25.
25. Cheng K, Rai P, Plagov A, Lan X, Kumar D, Salhan D, et al. Transplantation of bone marrow-derived MSCs improves cisplatinum-induced renal injury through paracrine mechanisms. Exp Mol Pathol 2013;94(3):466-73.
26. Lee EY, Xia Y, Kim WS, Kim MH, Kim TH, Kim KJ, et al. Hypoxia-enhanced wound-healing function of adipose-derived stem cells: increase in stem cell proliferation and up-regulation of VEGF and bFGF. Wound Repair Regen 2009;17(4):540-7.
27. Ranganath SH, Levy O, Inamdar MS, Karp JM. Harnessing the mesenchymal stem cell secretome for the treatment of cardiovascular disease. Cell Stem Cell 2012;10(3):244-58.
28. Li W, Ma N, Ong LL, Nesselmann C, Klopsch C, Ladilov Y, et al. Bcl-2 engineered MSCs inhibited apoptosis and improved heart function. Stem Cells 2007;25(8):2118-27.
29. Wisel S, Khan M, Kuppusamy ML, Mohan IK, Chacko SM, Rivera BK, et al. Pharmacological preconditioning of mesenchymal stem cells with trimetazidine (1-[2,3,4-trimethoxybenzyl]piperazine) protects hypoxic cells against oxidative stress and enhances recovery of myocardial function in infarcted heart through Bcl-2 expression. J Pharmacol Exp Ther 2009;329:543-50.
30. Herrmann JL, Wang Y, Abarbanell AM, Weil BR, Tan JN, Meldrum DR. Preconditioning mesenchymal stem cells with transforming growth factor-alpha improves mesenchymal stem cell mediated cardioprotection. Shock 2010;33:24-30.
31. Haider H, Ashraf M. Strategies to promote donor cell survival: combining preconditioning approach with stem cell transplantation. J Mol Cell Cardiol 2008;45(4):554-66.
32. Osugi M, Katagiri W, Yoshimi R, Inukai T, Hibi H, Ueda M. Conditioned media from mesenchymal stem cells enhanced bone regeneration in rat calvarial bone defects. Tissue Eng A 2012;18(13-14):1479-89.
33. Bronckaers A, Hilkens P, Fanton Y, Struys T, Gervois P, Politis C, et al. Angiogenic properties of human dental pulp stem cells. PLOS ONE 2013;8(8):71104.
34. Tran-Hung L, Laurent P, Camps J, About I. Quantification of angiogenic growth factors released by human dental cells after injury. Arch Oral Biol 2008;53:9-13.
35. Pochampally RR, Smith JR, Ylostalo J, Prockop DJ. Serum deprivation of human marrow stromal cells (hMSCs) selects for a subpopulation of early progenitor cells with enhanced expression of OCT-4 and other embryonic genes. Blood 2004;103(5):1647-52.
36. Zhu W, Chen J, Cong X, Hu S, Chen X. Hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells. Stem Cells 2006;24(2):416-25.
37. Sanchez CG, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, et al. Activation of autophagy in mesenchymal stem cells provides tumor stromal support. Carcinogenesis 2011;32(7):964-72.
38. Mylotte LA, Duffy AM, Murphy M, O'Brien T, Samali A, Barry F, et al. Metabolic flexibility permits mesenchymal stem cell survival in an ischemic environment. Stem Cells 2008;26(5):1325-36.
39. Sanchez C, Oskowitz A, Pochampally RR. Epigenetic reprogramming of IGF1 and leptin genes by serum deprivation in multipotential mesenchymal stromal cells. Stem Cells 2009;27(2):375-82.
40. Hu L, Zhao J, Liu J, Gong N, Chen L. Effects of adipose stem cell-conditioned medium on the migration of vascular endothelial cells, fibroblasts and keratinocytes. Exp Ther Med 2013;5(3):701-6.
41. Melin M, Joffre-Romeas A, Farges JC, Couble ML, Magloire H, Bleicher F. Effects of TGFbeta1 on dental pulp cells in cultured human tooth slices. J Dent Res 2000;79(9):1689-96.
42. Mathieu S, Jeanneau C, Sheibat-Othman N, Kalaji N, Fessi H, About I. Usefulness of controlled release of growth factors in investigating the early events of dentin-pulp regeneration. J Endod 2013;39:228-35.
43. Nie X, Tian W, Zhang Y, Chen X, Dong R, Jiang M, et al. Induction of transforming growth factor-beta 1 on dentine pulp cells in different culture patterns. Cell Biol Int 2006;30:295-300.
44. Li Y, Lü X, Sun X, Bai S, Li S, Shi J. Odontoblast-like cell differentiation and dentin formation induced with TGF-b1. Arch Oral Biol 2011;56:1221-9.
45. Moghadasali R, Mutsaers HA, Azarnia M, Aghdami N, Baharvand H, Torensma R, et al. Mesenchymal stem cell-conditioned medium accelerates regeneration of human renal proximal tubule epithelial cells after gentamicin toxicity. Exp Toxicol Pathol 2013;65(5):595-600.
46. Bi B, Schmitt R, Israilova M, Nishio H, Cantley LG. Stromal cells protect against acute tubular injury via an endocrine effect. J Am Soc Nephrol 2007;18(9):2486-96.
47. Gerwins P, Sköldenberg E, Claesson-Welsh L. Function of fibroblast growth factors and vascular endothelial growth factors and their receptors in angiogenesis. Crit Rev Oncol Hematol 2000;34(3):185-94.
48. Kramer N, Walzl A, Unger C, Rosner M, Krupitza G, Hengstschläger M, et al. In vitro cell migration and invasion assays. Mutat Res 2013;752(1):10-24.
49. Peplow PV, Chatterjee MP. A review of the influence of growth factors and cytokines in in vitro human keratinocyte migration. Cytokine 2013;62(1):1-21.
50. Sloan AJ, Couble ML, Bleicher F, Magloire H, Smith AJ, Farges JC. Expression of TGF-beta receptors I and II in the human dental pulp by in situ hybridization. Adv Dent Res 2001;15:63-7.