Development of a hexahistidine-3× FLAG-tandem affinity purification method for endogenous protein complexes in Pichia pastoris

Journal of Structural and Functional Genomics

Volumn 15, Issue 4, 2014, Pages 191-199

  Higo, Toshiaki ^a,b,c   Suka, Noriyuki ^b,d   Ehara, Haruhiko ^b,c,e   Wakamori, Masatoshi ^b,c   Sato, Shin ^b,c   Maeda, Hideaki ^a,b,c   Sekine, Shun ichi ^b,c,e   Umehara, Takashi ^b,c   Yokoyama, Shigeyuki ^b,c,e  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

^b RIKEN Systems and Structural Biology Center   (Japan)

^c RIKEN   (Japan)

^d MEISEI UNIVERSITY   (Japan)

^e UNIVERSITY OF TOKYO   (Japan)

Author keywords

Epitope tagging; RNA polymerase; Transcription; Yeast

Indexed keywords

DNA DIRECTED RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE III; HEXAHISTIDINE; PROTEINASE; RNA POLYMERASE II; THROMBIN; FUNGAL PROTEIN; HISTIDINE; HYBRID PROTEIN; MULTIPROTEIN COMPLEX; POLYHISTIDINE;

AMINO ACID SEQUENCE; ARTICLE; CHROMOSOME; CRYSTALLIZATION; NONHUMAN; PICHIA PASTORIS; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN INTERACTION; PROTEIN PURIFICATION; TANDEM AFFINITY PURIFICATION; X RAY CRYSTALLOGRAPHY; AFFINITY CHROMATOGRAPHY; BIOSYNTHESIS; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PICHIA; PROCEDURES;

PICHIA PASTORIS;

CHROMATOGRAPHY, AFFINITY; FUNGAL PROTEINS; HISTIDINE; MULTIPROTEIN COMPLEXES; PICHIA; RECOMBINANT FUSION PROTEINS;

EID: 84911965107     PISSN: 1345711X     EISSN: 15700267     Source Type: Journal    
DOI: 10.1007/s10969-014-9190-1     Document Type: Article

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