Tandem affinity purification of the Candida albicans septin protein complex

Yeast

Volumn 21, Issue 12, 2004, Pages 1025-1033

  Kaneko, Aki ^a   Umeyama, Takashi ^a   Hanaoka, Nozomu ^a,b   Monk, Brian C ^c   Uehara, Yoshimasa ^a   Niimi, Masakazu ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^b TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^c UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

Candida albicans; Hyphae; Protein complex; Proteome; Septin; Yeast

Indexed keywords

CYTOSKELETON PROTEIN; EPITOPE; FUNGAL PROTEIN; HISTIDINE; PROTEIN CACDC10P; PROTEIN CACDC11P; PROTEIN CACDC12P; PROTEIN CACDC3P; PROTEIN CASEP7P; PROTEOME; SEPTIN; UNCLASSIFIED DRUG;

ARTICLE; CANDIDA ALBICANS; CELL STRUCTURE; CONTROLLED STUDY; ELECTROPHORESIS; FUNGAL STRAIN; FUNGUS GROWTH; FUNGUS HYPHAE; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; STOICHIOMETRY; TANDEM AFFINITY PURIFICATION; WESTERN BLOTTING;

AMINO ACID SEQUENCE; BASE SEQUENCE; CANDIDA ALBICANS; CHROMATOGRAPHY, AFFINITY; CYTOSKELETAL PROTEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; EPITOPES; FUNGAL PROTEINS; HISTIDINE; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PEPTIDES; PROTEOME; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

CANDIDA; CANDIDA ALBICANS; FUNGI;

EID: 6344280288     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.1147     Document Type: Article

References (22)

1. Casamayor A, Snyder M. 2003. Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function. Mol Cell Biol 23: 2762-2777.
2. Cubitt AB, Heim R, Adams SR, et al. 1995. Understanding, improving and using green fluorescent proteins. Trends Biochem Sci 20: 448-455.
3. DiDomenico BJ, Brown NH, Lupisella J, et al. 1994. Homologs of the yeast neck filament associated genes: isolation and sequence analysis of Candida albicans CDC3 and CDC10. Mol Gen Genet 242: 689-698.
4. Field J, Nikawa J, Broek D, et al. 1988. Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell Biol 8: 2159-2165.
5. Fonzi WA, Irwin MY. 1993. Isogenic strain construction and gene mapping in Candida albicans. Genetics 134: 717-728.
6. Frazier JA, Wong ML, Longtine MS, et al. 1998. Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function. J Cell Biol 143: 737-749.
7. Gavin AC, Bosche M, Krause R, et al. 2002. Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415: 141-147.
8. Gietz RD, Woods RA. 2001. Genetic transformation of yeast. Biotechniques 30: 816-820, 822-816, 828 passim.
9. Gola S, Martin R, Walther A, et al. 2003. New modules for PCR-based gene targeting in Candida albicans: rapid and efficient gene targeting using 100 bp of flanking homology region. Yeast 20: 1339-1347.
10. Hagan IM, Stirling CJ. 1998. Immunological approaches to the study of protein localisation in yeast. Methods Microbiol 26: 201-221.
11. Jones T, Federspiel NA, Chibana H, et al. 2004. The diploid genome sequence of Candida albicans. Proc Natl Acad Sci USA 101: 7329-7334.
12. Longtine M, Bi E. 2003. Regulation of septin organization and function in yeast. Trends Cell Biol 13: 403-409.
13. Mortensen EM, McDonald H, Yates J III, Kellogg DR. 2002. Cell cycle-dependent assembly of a Gin4-septin complex. Mol Biol Cell 13: 2091-2105.
14. Niimi M, Nagai Y, Niimi K, et al. 2002. Identification of two proteins induced by exposure of the pathogenic fungus Candida glabrata to fluconazole. J Chromatogr B Anal Technol Biomed Life Sci 782: 245-252.
15. Odds FC. 1994. Pathogenesis of Candida infections. J Am Acad Dermatol 31: S2-5.
16. Rigaut G, Shevchenko A, Rutz B, et al. 1999. A generic protein purification method for protein complex characterization and proteome exploration. Nature Biotechnol 17: 1030-1032.
17. Smith DB, Johnson KS. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67: 31-40.
18. Sudbery PE. 2001. The germ tubes of Candida albicans hyphae and pseudohyphae show different patterns of septin ring localization. Mol Microbiol 41: 19-31.
19. Umeyama T, Nagai Y, Niimi M, Uehara Y. 2002. Construction of FLAG tagging vectors for Candida albicans. Yeast 19: 611-618.
20. Warenda AJ, Kauffman S, Sherrill TP, et al. 2003. Candida albicans septin mutants are defective for invasive growth and virulence. Infect Immun 71: 4045-4051.
21. Warenda AJ, Konopka JB. 2002. Septin function in Candida albicans morphogenesis. Mol Biol Cell 13: 2732-2746.
22. Yanisch-Perron C, Vieira J, Messing J. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119.