Structural characterization of the HSP70 interaction domain of the hepatitis C viral protein NS5A

Virology

Volumn 475, Issue , 2015, Pages 46-55

  Khachatoorian, Ronik ^a   Ruchala, Piotr ^a   Waring, Alan ^b   Jung, Chun Ling ^a   Ganapathy, Ekambaram ^a   Wheatley, Nicole ^a   Sundberg, Christopher ^a   Arumugaswami, Vaithilingaraja ^a,c   Dasgupta, Asim ^a   French, Samuel W ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b HARBOR UCLA MEDICAL CENTER   (United States)

^c CEDARS SINAI MEDICAL CENTER   (United States)

Author keywords

HCV; HSP70; IRES; NS5A; Peptide; Protein binding

Indexed keywords

HEAT SHOCK PROTEIN 70; NONSTRUCTURAL PROTEIN 5A; PHENYLALANINE; SYNTHETIC PEPTIDE; TYROSINE; VALINE; NS-5 PROTEIN, HEPATITIS C VIRUS; VIRUS PROTEIN;

AMINO ACID ANALYSIS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANTIVIRAL ACTIVITY; ARTICLE; BETA SHEET; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CYCLIZATION; DRUG DETERMINATION; DRUG PROTEIN BINDING; DRUG SYNTHESIS; DRUG TARGETING; GENETIC CONSERVATION; HEPATITIS C VIRUS; HYDROPHOBICITY; IC50; INFRARED SPECTROSCOPY; MUTAGENESIS; NONHUMAN; PEPTIDE ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; RESIDUE ANALYSIS; STRUCTURE ANALYSIS; VIRUS ENTRY; CELL LINE; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; HEPACIVIRUS; HUMAN; METABOLISM; NUCLEOTIDE SEQUENCE; PLASMID; PROTEIN CONFORMATION; PROTEIN TERTIARY STRUCTURE; SURFACE PLASMON RESONANCE;

HEPATITIS C VIRUS;

AMINO ACID SUBSTITUTION; BINDING SITES; CELL LINE; CONSERVED SEQUENCE; HEPACIVIRUS; HSP70 HEAT-SHOCK PROTEINS; HUMANS; MODELS, MOLECULAR; PLASMIDS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; SURFACE PLASMON RESONANCE; VIRAL NONSTRUCTURAL PROTEINS;

EID: 84911881810     PISSN: 00426822     EISSN: 10960341     Source Type: Journal    
DOI: 10.1016/j.virol.2014.10.011     Document Type: Article

References (36)

1. Arumugaswami V., Remenyi R., Kanagavel V., Sue E.Y., Ngoc Ho T., Liu C., Fontanes V., Dasgupta A., Sun R. High-resolution functional profiling of hepatitis C virus genome. PLoS Pathog. 2008, 4:e1000182.
2. Baron S. Medical Microbiology 1996, University of Texas Medical Branch at Galveston, Galveston, TX. 4th ed.
3. Byler D.M., Susi H. Examination of the secondary structure of proteins by deconvolved FTIR spectra. Biopolymers 1986, 25:469-487.
4. Chelli R., Gervasio F.L., Procacci P., Schettino V. Stacking and T-shape competition in aromatic-aromatic amino acid interactions. J. Am. Chem. Soc. 2002, 124:6133-6143.
5. Ciesek S., Manns M.P. Hepatitis in 2010: the dawn of a new era in HCV therapy. Nat. Rev. Gastroenterol. Hepatol. 2011, 8:69-71.
6. Dwivedi A.M., Krimm S. Vibrational analysis of peptides, polypeptides, and proteins. XVIII. Conformational sensitivity of the alpha-helix spectrum: alpha I- and alpha II-poly(l-alanine). Biopolymers 1984, 23:923-943.
7. El-Serag H.B. Hepatocellular carcinoma: an epidemiologic view. J. Clin. Gastroenterol. 2002, 35:S72-S78.
8. Fields G.B., Noble R.L. Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids. Int. J. Pept. Protein Res. 1990, 35:161-214.
9. Gonzalez O., Fontanes V., Raychaudhuri S., Loo R., Loo J., Arumugaswami V., Sun R., Dasgupta A., French S.W. The heat shock protein inhibitor Quercetin attenuates hepatitis C virus production. Hepatology 2009, 50:1756-1764.
10. He Y., Yan W., Coito C., Li Y., Gale M., Katze M.G. The regulation of hepatitis C virus (HCV) internal ribosome-entry site-mediated translation by HCV replicons and nonstructural proteins. J. Gen. Virol. 2003, 84:535-543.
11. Hess B., Kutzner C., van der Spoel D., Lindahl E. GROMACS 4: algorithms for highly efficient, load-balanced, and scalable molecular simulation. J. Chem. Theory Comput. 2008, 4:435-447.
12. Hughes M., Griffin S., Harris M. Domain III of NS5A contributes to both RNA replication and assembly of hepatitis C virus particles. J. Gen. Virol. 2009, 90:1329-1334.
13. Kabsch W., Sander C. Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features. Biopolymers 1983, 22:2577-2637.
14. Khachatoorian R., Arumugaswami V., Raychaudhuri S., Yeh G.K., Maloney E.M., Wang J., Dasgupta A., French S.W. Divergent antiviral effects of bioflavonoids on the hepatitis C virus life cycle. Virology 2012, 433:346-355.
15. Khachatoorian R., Arumugaswami V., Ruchala P., Raychaudhuri S., Maloney E.M., Miao E., Dasgupta A., French S.W. A cell-permeable hairpin peptide inhibits hepatitis C viral nonstructural protein 5A-mediated translation and virus production. Hepatology 2012, 55:1662-1672.
16. Khachatoorian R., Ganapthy E., Ahmadieh Y., Wheatley N., Sundberg C., Jung C.-L., Arumugaswami V., Raychaudhuri S., Dasgupta A., French S.W. The NS5A-binding heat shock proteins HSC70 and HSP70 play distinct roles in the hepatitis C viral life cycle. Virology 2014, 454-455:118-127.
17. Kumar S., Nussinov R. Salt bridge stability in monomeric proteins. J. Mol. Biol. 1999, 293:1241-1255.
18. Lawitz E.J., Gruener D., Hill J.M., Marbury T., Moorehead L., Mathias A., Cheng G., Link J.O., Wong K.A., Mo H., McHutchison J.G., Brainard D.M. A phase 1, randomized, placebo-controlled, 3-day, dose-ranging study of GS-5885, an NS5A inhibitor, in patients with genotype 1 hepatitis C. J. Hepatol. 2012, 57:24-31.
19. Lim Y.S., Shin K.S., Oh S.H., Kang S.M., Won S.J., Hwang S.B. Nonstructural 5A protein of hepatitis C virus regulates heat shock protein 72 for its own propagation. J. Viral Hepat. 2012, 19:353-363.
20. Lindenbach B.D., Rice C.M. Unravelling hepatitis C virus replication from genome to function. Nature 2005, 436:933-938.
21. Love R.A., Brodsky O., Hickey M.J., Wells P.A., Cronin C.N. Crystal structure of a novel dimeric form of NS5A domain I protein from hepatitis C virus. J. Virol. 2009, 83:4395-4403.
22. Mayer M.P., Bukau B. Hsp70 chaperones: cellular functions and molecular mechanism. Cell. Mol. Life Sci. 2005, 62:670-684.
23. McGaughey G.B., Gagne M., Rappe A.K. pi-Stacking interactions. Alive and well in proteins. J. Biol. Chem. 1998, 273:15458-15463.
24. Moradpour D., Penin F., Rice C.M. Replication of hepatitis C virus. Nat. Rev. Microbiol. 2007, 5:453-463.
25. Pacheco A., Martinez-Salas E. Insights into the biology of IRES elements through riboproteomic approaches. J. Biomed. Biotechnol. 2010, 2010:458927.
26. Parent R., Qu X., Petit M.A., Beretta L. The heat shock cognate protein 70 is associated with hepatitis C virus particles and modulates virus infectivity. Hepatology 2009, 49:1798-1809.
27. Shepard C.W., Finelli L., Alter M.J. Global epidemiology of hepatitis C virus infection. Lancet Infect. Dis. 2005, 5:558-567.
28. Sofia M.J., Bao D., Chang W., Du J., Nagarathnam D., Rachakonda S., Reddy P.G., Ross B.S., Wang P., Zhang H.R., Bansal S., Espiritu C., Keilman M., Lam A.M., Steuer H.M., Niu C., Otto M.J., Furman P.A. Discovery of a beta-d-2'-deoxy-2'-alpha-fluoro-2'-beta-C-methyluridine nucleotide prodrug (PSI-7977) for the treatment of hepatitis C virus. J. Med. Chem. 2010, 53:7202-7218.
29. Tadesse L., Nazarbaghi R., Walters L. Isotopically enhanced infrared spectroscopy: a novel method for examining secondary structure at specific sites in conformationally heterogeneous peptides. J. Am. Chem. Soc. 1991, 113:7036-7037.
30. Tellinghuisen T.L., Foss K.L., Treadaway J.C., Rice C.M. Identification of residues required for RNA replication in domains II and III of the hepatitis C virus NS5A protein. J. Virol. 2008, 82:1073-1083.
31. Tellinghuisen T.L., Marcotrigiano J., Rice C.M. Structure of the zinc-binding domain of an essential component of the hepatitis C virus replicase. Nature 2005, 435:374-379.
32. Vasconcelos D.Y., Cai X.H., Oglesbee M.J. Constitutive overexpression of the major inducible 70kDa heat shock protein mediates large plaque formation by measles virus. J. Gen. Virol. 1998, 79(Pt 9):2239-2247.
33. Wang C., Sarnow P., Siddiqui A. Translation of human hepatitis C virus RNA in cultured cells is mediated by an internal ribosome-binding mechanism. J. Virol. 1993, 67:3338-3344.
34. Weeks S.A., Miller D.J. The heat shock protein 70 cochaperone YDJ1 is required for efficient membrane-specific flock house virus RNA replication complex assembly and function in Saccharomyces cerevisiae. J. Virol. 2008, 82:2004-2012.
35. Wheatley N.M., Gidaniyan S.D., Liu Y., Cascio D., Yeates T.O. Bacterial microcompartment shells of diverse functional types possess pentameric vertex proteins. Protein Sci. 2013, 22:660-665.
36. Zheng Z.Z., Miao J., Zhao M., Tang M., Yeo A.E., Yu H., Zhang J., Xia N.S. Role of heat-shock protein 90 in hepatitis E virus capsid trafficking. J. Gen. Virol. 2010, 91:1728-1736.