A synthetic O2-tolerant butanol pathway exploiting native fatty acid biosynthesis in Escherichia coli

Biotechnology and Bioengineering

Volumn 112, Issue 1, 2015, Pages 120-128

  Pásztor, András ^a   Kallio, Pauli ^a   Malatinszky, Dávid ^b   Akhtar, M Kalim ^a,c   Jones, Patrik R ^a,b  

^a UNIVERSITY OF TURKU   (Finland)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

^c UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Biofuel; Butanol; Escherichia coli; Fatty acid biosynthesis; Synthetic pathway

Indexed keywords

ALDEHYDES; BIOCHEMISTRY; BIOFUELS; BIOSYNTHESIS; BUTENES; CLOSTRIDIUM; ENZYME ACTIVITY; ESCHERICHIA COLI; PLANTS (BOTANY);

ACYL-ACP THIOESTERASES; BACTEROIDES FRAGILIS; CLOSTRIDIUM ACETOBUTYLICUM; CLOSTRIDIUM SPECIES; EQUIVALENT CONDITION; FATTY ACID BIOSYNTHESIS; SEQUENTIAL REDUCTIONS; SYNTHETIC PATHWAYS;

FATTY ACIDS;

ALDEHYDE REDUCTASE; BUTANOL; COENZYME A; OXYGEN; THIOL ESTER HYDROLASE; BIOFUEL; FATTY ACID;

ARTICLE; BACTERIAL METABOLISM; BACTEROIDES FRAGILIS; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; GROWTH; NONHUMAN; PHYSICAL PARAMETERS; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; STOICHIOMETRY; BIOSYNTHESIS; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES;

ESCHERICHIA COLI;

BIOFUELS; BUTANOLS; ESCHERICHIA COLI; FATTY ACIDS; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS;

EID: 84911443214     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25324     Document Type: Article

References (41)

1. Ahmad AL, Yasin NHM, Derek CJC, Lim JK. 2011. Microalgae as a sustainable energy source for biodiesel production: A review. Renew Sustain Energ Rev 15(1):584-593.
2. Akhtar MK, Turner NJ, Jones PR. 2013. Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities. Proc Natl Acad Sci 110(1):87-92.
3. Atsumi S, Cann AF, Connor MR, Shen CR, Smith KM, Brynildsen MP, Chou KJY, Hanai T, Liao JC. 2008. Metabolic engineering of Escherichia coli for 1-butanol production. Eng Metab Pathways Biofuels Prod 10(6):305-311.
4. Bond-Watts BB, Bellerose RJ, Chang MC. 2011. Enzyme mechanism as a kinetic control element for designing synthetic biofuel pathways. Nat Chem Biol 7(4):222-227.
5. Cooksley CM, Zhang Y, Wang H, Redl S, Winzer K, Minton NP. 2012. Targeted mutagenesis of the Clostridium acetobutylicum acetone-butanol-ethanol fermentation pathway. Metab Eng 14(6):630-641.
6. Dellomonaco C, Clomburg JM, Miller EN, Gonzalez R. 2011. Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals. Nature 476(7360):355-359.
7. Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson B. 2007. A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information. Mol Syst Biol 3:121.
8. Fortman JL, Chhabra S, Mukhopadhyay A, Chou H, Lee TS, Steen E, Keasling JD. 2008. Biofuel alternatives to ethanol: Pumping the microbial well. Trends Biotechnol 26(7):375-381.
9. Gurobi Optimization I. 2013. Gurobi Optimizer Reference Manual.
10. Hou X, Peng W, Xiong L, Huang C, Chen X, Chen X, Zhang W. 2013. Engineering Clostridium acetobutylicum for alcohol production. J Biotechnol 166(1-2):25-33.
11. Jang YS, Lee JY, Lee J, Park JH, Im JA, Eom MH, Lee SH, Song H, Cho JH, Seung do Y, Lee SY. 2012. Enhanced butanol production obtained by reinforcing the direct butanol-forming route in Clostridium acetobutylicum. MBio 3: (5):1-9.
12. Jin C, Yao M, Liu H, Lee C-fF, Ji J. 2011. Progress in the production and application of n-butanol as a biofuel. Renew Sustain Energ Rev 15(8):4080-4106.
13. Jing F, Cantu DC, Tvaruzkova J, Chipman JP, Nikolau BJ, Yandeau-Nelson MD, Reilly PJ. 2011. Phylogenetic and experimental characterization of an acyl-ACP thioesterase family reveals significant diversity in enzymatic specificity and activity. BMC Biochem 12(44):1-16.
14. Jones DT, Woods DR. 1986. Acetone-butanol fermentation revisited. Microbiol Rev 50(4):484-524.
15. Kallio P, Pásztor A, Thiel K, Akhtar MK, Jones PR. 2014. Renewable propane-Microbial biosynthesis and separation of an engine-ready hydrocarbon fuel. Nat Commun (In Press).
16. Lan EI, Liao JC. 2011. Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide. Metab Eng 13(4):353-363.
17. Lan EI, Liao JC. 2012. ATP drives direct photosynthetic production of 1-butanol in cyanobacteria. Proc Natl Acad Sci USA 109(16):6018-6023.
18. Lan EI, Ro SY, Liao JC. 2013. Oxygen-tolerant coenzyme A-acylating aldehyde dehydrogenase facilitates efficient photosynthetic n-butanol biosynthesis in cyanobacteria. Energy Environ Sci 6(9):2672-2681.
19. Lee SY, Park JH, Jang SH, Nielsen LK, Kim J, Jung KS. 2008. Fermentative butanol production by Clostridia. Biotechnol Bioeng 101(2):209-228.
20. Liu D, Chen Y, Li A, Ding F, Zhou T, He Y, Li B, Niu H, Lin X, Xie J., et al. 2013. Enhanced butanol production by modulation of electron flow in Clostridium acetobutylicum B3 immobilized by surface adsorption. Bioresour Technol 129(0):321-328.
21. Liu H, Yu C, Feng D, Cheng T, Meng X, Liu W, Zou H, Xian M. 2012. Production of extracellular fatty acid using engineered Escherichia coli. Microb Cell Factories 11(41):1-13.
22. Lu X, Vora H, Khosla C. 2008. Overproduction of free fatty acids in E. coli: Implications for biodiesel production. Eng Metab Pathways Biofuels Prod 10(6):333-339.
23. Mahadevan R, Edwards JS, Doyle FJ 3rd. 2002. Dynamic flux balance analysis of diauxic growth in Escherichia coli. Biophys J 83(3):1331-1340.
24. MATLAB. 2011. MATLAB and Statistics Toolbox Release R2011b, Natick, Massachusetts: The MathWorks Inc.
25. Mellan I. 1950. Industrial solvents, New York: Van Nostrand Reinhold. Vol. 1: p 482-488.
26. Na D, Yoo SM, Chung H, Park H, Park JH, Lee SY. 2013. Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nat Biotechnol 31(2):170-174.
27. Rodriguez GM, Atsumi S. 2012. Isobutyraldehyde production from Escherichia coli by removing aldehyde reductase activity. Microb Cell Factories 11: 90-2859-11-90.
28. Schellenberger J, Que R, Fleming RM, Thiele I, Orth JD, Feist AM, Zielinski DC, Bordbar A, Lewis NE, Rahmanian S., et al. 2011. Quantitative prediction of cellular metabolism with constraint-based models: The COBRA Toolbox v2.0. Nat Protoc 6(9):1290-1307.
29. Schenk PM, Thomas-Hall SR, Stephens E, Marx UC, Mussgnug JH, Posten C, Kruse O, Hankamer B. 2008. Second generation biofuels: High-efficiency microalgae for biodiesel production. BioEnergy Res 1(1):20-43.
30. Shen CR, Lan EI, Dekishima Y, Baez A, Cho KM, Liao JC. 2011. Driving forces enable high-titer anaerobic 1-butanol synthesis in Escherichia coli. Appl Environ Microbiol 77(9):2905-2915.
31. Sillers R, Chow A, Tracy B, Papoutsakis ET. 2008. Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance. Eng Metab Pathways Biofuels Prod 10(6):321-332.
32. Steen EJ, Chan R, Prasad N, Myers S, Petzold CJ, Redding A, Ouellet M, Keasling JD. 2008. Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol. Microb Cell Factories 7: 36-2859-7-36.
33. Tornatore C, Marchitto L, Mazzei A, Valentino G, Corcione FE, Merola SS. 2011. Effect of butanol blend on in-cylinder combustion process. J KONES Powertrain Transp 18(2):1-12.
34. Varma A, Palsson BO. 1994. Stoichiometric flux balance models quantitatively predict growth and metabolic by-product secretion in wild-type Escherichia coli W3110. Appl Environ Microbiol 60(10):3724-3731.
35. Varman AM, Xiao Y, Pakrasi HB, Tang YJ. 2013. Metabolic engineering of Synechocystis sp. strain PCC 6803 for isobutanol production. Appl Environ Microbiol 79(3):908-914.
36. Vidal R, López-Maury L, Guerrero MG, Florencio FJ. 2009. Characterization of an alcohol dehydrogenase from the Cyanobacterium Synechocystis sp. Strain PCC 6803 that responds to environmental stress conditions via the Hik34-Rre1 two-component system. J Bacteriol 191(13):4383-4391.
37. Yu M, Zhang Y, Tang IC, Yang S-T. 2011. Metabolic engineering of Clostridium tyrobutyricum for n-butanol production. Metab Eng 13(4):373-382.
38. Zelcbuch L, Antonovsky N, Bar-Even A, Levin-Karp A, Barenholz U, Dayagi M, Liebermeister W, Flamholz A, Noor E, Amram S., et al. 2013. Spanning high-dimensional expression space using ribosome-binding site combinatorics. Nucl Acids Res 41(9):e98.
39. Zhang F, Ouellet M, Batth TS, Adams PD, Petzold CJ, Mukhopadhyay A, Keasling J. 2012. Enhancing fatty acid production by the expression of the regulatory transcription factor FadR. Metab Eng 14(6):653-660.
40. Zheng YN, Li LZ, Xian M, Ma YJ, Yang JM, Xu X, He DZ. 2009. Problems with the microbial production of butanol. J Indust Microbiol Biotechnol 36(9):1127-1138.
41. Zverlov V, Berezina O, Velikodvorskaya G, Schwarz W. 2006. Bacterial acetone and butanol production by industrial fermentation in the Soviet Union: Use of hydrolyzed agricultural waste for biorefinery, Berlin/Heidelberg: Springer. p 587-597.