Lipase immobilization on magnetic microspheres via spacer arms: Effect of steric hindrance on the activity

Biotechnology and Bioprocess Engineering

Volumn 19, Issue 5, 2014, Pages 838-843

  Zhang, Dong Hao ^a   Li, Ya Qiong ^a   Peng, Li Juan ^a   Chen, Na ^a  

^a HEBEI UNIVERSITY   (China)

Author keywords

lipase immobilization; magnetic microspheres; poly(ethylene glycol) 800; spacer arm; steric hindrance

Indexed keywords

ALIPHATIC COMPOUNDS; CHEMICAL REACTIONS; ENZYME ACTIVITY; ETHYLENE GLYCOL; MAGNETISM; MICROSPHERES; POLYETHYLENE GLYCOLS; POLYOLS; STYRENE;

AVERAGE DIAMETER; IMMOBILIZE LIPASE; IMMOBILIZED LIPASE; LIPASE IMMOBILIZATION; MAGNETIC MICROSPHERES; SPACER ARM; STERIC HINDRANCES; STYRENE-ACRYLIC ACID;

SHIMS;

ETHYLENEDIAMINE; MACROGOL 400; MACROGOL 4000; MICROSPHERE; POLY(STYRENE ACRYLIC ACID)MAGNETIC MICROSPHERE; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL MODIFICATION; EMULSION; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; POLYMERIZATION; STEREOSPECIFICITY;

EID: 84911387750     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-013-0495-x     Document Type: Article

References (25)

1. Carrea, G. and S. Riva (2000) Properties and synthetic applications of enzymes in organic solvents. Angew. Chem. Int. Edit. 39: 2226–2254.
2. Reetz, M. T. (2002) Lipases as practical biocatalysts. Curr. Opin. Chem. Biol. 6: 145–150.
3. Gao, S. L., Y. J. Wang, W. W. Wang, G. S. Luo, and Y. Y. Dai (2010) Enhancing performance of lipase immobilized on methylmodified silica aerogels at the adsorption and catalysis processes: Effect of co-solvents. J. Mol. Catal. B: Enz. 62: 218–224.
4. Chen, B., M. E. Miller, and R. A. Gross (2007) Effects of porous polystyrene resin parameters on Candida Lipase B adsorption, distribution, and polyester synthesis activity. Langmuir 23: 6467–6474.
5. Fernandez-Lorente, G., Z. Cabrera, C. Godoy, R. Fernandez-Lafuente, J. M. Palomo, and J. M. Guisan (2008) Interfacially activated lipases against hydrophobic supports: Effect of the support nature on the biocatalytic properties. Proc. Biochem. 43: 1061–1067.
6. Li, Y., F. Gao, W. Wei, J. B. Qu, G. H. Ma, and W. Q. Zhou (2010) Pore size of macroporous polystyrene microspheres affects lipase immobilization. J. Mol. Catal. B: Enz. 66: 182–189.
7. Ge, J., D. N. Lu, J. Wang, and Z. Liu (2009) Lipase nanogel catalyzed transesterification in anhydrous dimethyl sulfoxide. Biomacromol. 10: 1612–1618.
8. Wang, Y. J. and F. Caruso (2005) Mesoporous silica spheres as supports for enzyme immobilization and encapsulation. Chem. Mater. 17: 953–961.
9. Bilkova, Z., M. Slovakova, N. Minc, C. Futterer, R. Cecal, D. Horak, M. Benes, I.I. Potier, J. Krenkova, M. Przybylski, and J. L. Viovy (2006) Functionalized magnetic micro- and nanoparticles: Optimization and application to μ-chip tryptic digestion. Electrophor. 27: 1811–1824.
10. Bruice, T. C. and P. Y. Bruice (2005) Covalent intermediates and enzyme proficiency. J. Am. Chem. Soc. 127: 12478–12479.
11. Carolan, N., R. J. Forster, and C. O. Fagain (2007) Covalent attachment of ferrocene to soybean peroxidase glycans: Electron transfer mediation to redox enzymes. Bioconjugate Chem. 18: 524–529.
12. Chiou, S. H. and W. T. Wu (2004) Immobilization of Candida rugosa lipase on chitosan with activation of the hydroxyl groups. Biomaterials 25: 197–204.
13. Knezevic, Z., N. Milosavic, D. Bezbradica, Z. Jakovljevic, and R. Prodanovic (2006) Immobilization of lipase from Candida rugosa on Eupergit® supports by covalent attachment. Biochem. Eng. J. 30: 269–278.
14. Das, R. D., S. Maji, S. Das, and C. R. Chaudhuri (2010) Optimization of covalent antibody immobilization on macroporous silicon solid supports. Appl. Surf. Sci. 256: 5867–5875.
15. Tsai, H. C. and R. A. Doong (2007) Preparation and characterization of urease-encapsulated biosensors in poly (vinyl alcohol)-modified silica sol-gel materials. Biosens. Bioelectron. 23: 66–73.
16. Tischer, W. and V. Kasche (1999) Immobilized enzymes: Crystals or carriers? Trends Biotechnol. 17: 326–335.
17. Zhang, D.-H., L.-X. Yuwen, C. Li, and Y.-Q. Li (2012) Effect of poly(vinyl acetate-acrylamide) microspheres properties and steric hindrance on the immobilization of Candida rugosa lipase. Bioresour. Technol. 124: 233–236.
18. Wang, F., Z Gu, Z. Cui, and L. Liu (2011) Comparison of covalent immobilization of amylase on polystyrene pellets with pentaethylenehexamine and pentaethylene glycol spacers. Bioresour. Technol. 102: 9374–9379.
19. Hinterwirth, H., W. Lindner, and M. Lammerhofer (2012) Bioconjugation of trypsin onto gold nanoparticles: Effect of surface chemistry on bioactivity. Anal. Chim. Acta 733: 90–97.
20. Manta, C., N. Ferraz, L. Betancor, G. Antunes, F. Batista-Viera, J. Carlsson, and K. Caldwell (2003) Polyethylene glycol as a spacer for solid-phase enzyme immobilization. Enz. Microb. Technol. 33: 890–898.
21. Zhang, D.-H., L.-X. Yuwen, Y.-L. Xie, W. Li, and X.-B. Li (2012) Improving immobilization of lipase onto magnetic microspheres with moderate hydrophobicity/hydrophilicity. Colloid. Surface. B. 89: 73–78.
22. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
23. Yong, Y., Y. X. Bai, Y. F. Li, L. Lin, Y. J. Cui, and C. G. Xia (2008) Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity. Proc. Biochem. 43: 1179–1185.
24. El-Sherif, H., P. L. Martelli, R. Casadio, M. Portaccio, U. Bencivenga, and D. G. Mita (2001) Urease immobilization on chemically grafted nylon membranes Part 1: Isothermal characterization. J. Mol. Catal. B: Enz. 14: 15–29.
25. Bhardwaj, A., J. Lee, K. Glauner, S. Ganapathi, D. Bhattacharyya, and D. A. Butterfield (1996) Biofunctional membranes: An EPR study of active site structure and stability of papain noncovalently immobilized on the surface of modified poly(ether) sulfone membranes through the avidin-biotin linkage. J. Membrane Sci. 119: 241–252.