Single-cell western blotting after whole-cell imaging to assess cancer chemotherapeutic response

Analytical Chemistry

Volumn 86, Issue 20, 2014, Pages 10429-10436

  Kang, Chi Chih ^a   Lin, Jung Ming G ^a,b   Xu, Zhuchen ^a   Kumar, Sanjay ^a,b   Herr, Amy E ^a,b  

^a University of California at Berkeley   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; CELL CULTURE; CELL PROLIFERATION; CELLS; CHEMICAL ANALYSIS; DISEASES; DRUG THERAPY; ELECTROPHORESIS; GLYCOPROTEINS; MOLECULAR BIOLOGY; PROTEINS; TUMORS;

CELL-TO-CELL VARIATION; GLIOBLASTOMA CELLS; MULTIDRUG RESISTANTS; PERSONALIZED MEDICINES; POLYACRYLAMIDE GEL ELECTROPHORESIS; POTENTIAL MECHANISM; PROTEIN ELECTROPHORESIS; TARGETED PROTEOMICS;

CYTOLOGY;

ANTINEOPLASTIC AGENT;

APOPTOSIS; GLIOBLASTOMA; HUMAN; SINGLE CELL ANALYSIS; TUMOR CELL LINE; TWO DIMENSIONAL GEL ELECTROPHORESIS; WESTERN BLOTTING;

ANTINEOPLASTIC AGENTS; APOPTOSIS; BLOTTING, WESTERN; CELL LINE, TUMOR; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GLIOBLASTOMA; HUMANS; SINGLE-CELL ANALYSIS;

EID: 84910665930     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac502932t     Document Type: Article

References (44)

1. Niepel, M.; Spencer, S. L.; Sorger, P. K. Curr. Opin Chem. Biol. 2009, 13, 556-561
2. Cohen, A. A.; Geva-Zatorsky, N.; Eden, E.; Frenkel-Morgenstern, M.; Issaeva, I.; Sigal, A.; Milo, R.; Cohen-Saidon, C.; Liron, Y.; Kam, Z.; Cohen, L.; Danon, T.; Perzov, N.; Alon, U. Science (New York, N.Y) 2008, 322, 1511-1516
3. Fisher, R.; Pusztai, L.; Swanton, C. Br. J. Cancer 2013, 108, 479-485
4. Gascoigne, K. E.; Taylor, S. S. Cancer Cell 2008, 14, 111-122
5. Vrignaud, P.; Londos-Gagliardi, D.; Robert, J. Oncology 1986, 43, 60-66
6. Huet, S.; Schott, B.; Robert, J. Br. J. Cancer 1992, 65, 538-544
7. Zanotto-Filho, A.; Braganhol, E.; Schroder, R.; de Souza, L. H.; Dalmolin, R. J.; Pasquali, M. A.; Gelain, D. P.; Battastini, A. M.; Moreira, J. C. Biochem. Pharmacol. 2011, 81, 412-424
8. Eisele, G.; Weller, M. Cancer Lett. 2013, 332, 335-345
9. Edwards, B. S.; Young, S. M.; Saunders, M. J.; Bologa, C.; Oprea, T. I.; Ye, R. D.; Prossnitz, E. R.; Graves, S. W.; Sklar, L. A. Exp. Opin. Drug Discovery 2007, 2, 685-696
10. O'Hara, D. M.; Xu, Y.; Liang, Z.; Reddy, M. P.; Wu, D. Y.; Litwin, V. J. Immunol. Methods 2011, 363, 120-134
11. O'Brien, P. J.; Lee, M.; Spilker, M. E.; Zhang, C. C.; Yan, Z.; Nichols, T. C.; Li, W.; Johnson, C. H.; Patti, G. J.; Siuzdak, G. Cancer Metab. 2013, 1, 4
12. Sarkar, A.; Kolitz, S.; Lauffenburger, D. A.; Han, J. Nat. Commun. 2014, 5, 3421
13. Maecker, H. T.; Trotter, J. Cytometry A 2006, 69, 1037-1042
14. Stadler, C.; Rexhepaj, E.; Singan, V. R.; Murphy, R. F.; Pepperkok, R.; Uhlen, M.; Simpson, J. C.; Lundberg, E. Nat. Methods 2013, 10, 315-323
15. Hughes, A. J.; Spelke, D. P.; Xu, Z.; Kang, C. C.; Schaffer, D. V.; Herr, A. E. Nat. Methods 2014, 11, 749-755
16. Hughes, A. J.; Herr, A. E. Proc. Natl. Acad. Sci. U.S.A. 2012, 109, 21450-21455
17. Hughes, A. J.; Lin, R. K.; Peehl, D. M.; Herr, A. E. Proc. Natl. Acad. Sci. U.S.A. 2012, 109, 5972-5977
18. Stepanenko, A. A.; Kavsan, V. M. Gene 2014, 540, 263-265
19. Shapiro, A. L.; Vinuela, E.; Maizel, J. V., Jr. Biochem. Biophys. Res. Commun. 1967, 28, 815-820
20. Gallagher, S. R. In Current Protocols in Molecular Biology Ausubel, F. M., Eds.; John Wiley & Sons: New York, 2012; Chapter 10, Unit 10 12A.
21. Lo, C. T.; Throckmorton, D. J.; Singh, A. K.; Herr, A. E. Lab Chip 2008, 8, 1273-1279
22. Hansma, H. G. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 14678-14680
23. Stellwagen, N. C. Electrophoresis 1998, 19, 1542-1547
24. Tong, J.; Anderson, J. L. Biophys. J. 1996, 70, 1505-1513
25. Aleman, C.; Annereau, J. P.; Liang, X. J.; Cardarelli, C. O.; Taylor, B.; Yin, J. J.; Aszalos, A.; Gottesman, M. M. Cancer Res. 2003, 63, 3084-3091
26. Henjes, F.; Bender, C.; von der Heyde, S.; Braun, L.; Mannsperger, H. A.; Schmidt, C.; Wiemann, S.; Hasmann, M.; Aulmann, S.; Beissbarth, T.; Korf, U. Oncogenesis 2012, 1, e16
27. Na, S.; Collin, O.; Chowdhury, F.; Tay, B.; Ouyang, M.; Wang, Y.; Wang, N. Proc. Natl. Acad. Sci. U.S.A. 2008, 105, 6626-6631
28. Rezaei Tavirani, M.; Moghaddamnia, S. H.; Ranjbar, B.; Amani, M.; Marashi, S. A. J. Biochem. Mol. Biol. 2006, 39, 530-536
29. Turi, A.; Lu, R. C.; Lin, P. S. Biochem. Biophys. Res. Commun. 1981, 100, 584-590
30. Flora, K.; Brennan, J. D.; Baker, G. A.; Doody, M. A.; Bright, F. V. Biophys. J. 1998, 75, 1084-1096
31. Mazzola, J. L.; Sirover, M. A. Biochim. Biophys. Acta 2003, 1622, 50-56
32. Ku, T.; Lu, P.; Chan, C.; Wang, T.; Lai, S.; Lyu, P.; Hsiao, N. Comput. Biol. Chem. 2009, 33, 445-450
33. Saeed, I. A.; Ashraf, S. S. Int. J. Biol. Macromol. 2009, 45, 236-241
34. Alkaabi, K. M.; Yafea, A.; Ashraf, S. S. Appl. Biochem. Biotechnol. 2005, 126, 149-156
35. Zimmer, M. Chem. Rev. 2002, 102, 759-781
36. Wesselborg, S.; Engels, I. H.; Rossmann, E.; Los, M.; Schulze-Osthoff, K. Blood 1999, 93, 3053-3063
37. Haar, C. P.; Hebbar, P.; Wallace, G. C. t.; Das, A.; Vandergrift, W. A., 3rd; Smith, J. A.; Giglio, P.; Patel, S. J.; Ray, S. K.; Banik, N. L. Neurochem. Res. 2012, 37, 1192-1200
38. Spiegl-Kreinecker, S.; Buchroithner, J.; Elbling, L.; Steiner, E.; Wurm, G.; Bodenteich, A.; Fischer, J.; Micksche, M.; Berger, W. J. Neurooncol. 2002, 57, 27-36
39. Trock, B. J.; Leonessa, F.; Clarke, R. J. Natl. Cancer Inst. 1997, 89, 917-931
40. Yardley, D. A. Int. J. Breast Cancer 2013, 2013, 137414
41. The Cancer Genome Atlas Network. Nature 2012, 490, 61-70
42. The Cancer Genome Atlas Research Network. Nature 2008, 455, 1061-1068
43. Boja, E. S.; Rodriguez, H. Clin. Proteomics 2014, 11, 22
44. Waters, K. M.; Liu, T.; Quesenberry, R. D.; Willse, A. R.; Bandyopadhyay, S.; Kathmann, L. E.; Weber, T. J.; Smith, R. D.; Wiley, H. S.; Thrall, B. D. PLoS One 2012, 7, e34515