CRISPR/Cas9 mediates efficient conditional mutagenesis in Drosophila

G3: Genes, Genomes, Genetics

Volumn 4, Issue 11, 2014, Pages 2167-2173

  Xue, Zhaoyu ^a   Wu, Menghua ^a   Wen, Kejia ^a   Ren, Menda ^a   Long, Li ^a   Zhang, Xuedi ^a   Gao, Guanjun ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

Conditional mutagenesis; CRISPR Cas9; Drosophila; Gal4; gRNA

Indexed keywords

GUIDE RNA;

ARTICLE; BAM GENE; CID GENE; CRISPR CAS SYSTEM; CRISPR CAS9 GENE; DROSOPHILA; GENE; GENE EXPRESSION; GENE MUTATION; MS(3)K81 GENE; NONHUMAN; NOS GENE; OVARY; RNA INTERFERENCE; TESTIS; TRANSGENE; ANIMAL; ANTIBODY SPECIFICITY; GENE TARGETING; GENETICS; MUTAGENESIS; PROCEDURES;

ANIMALS; CRISPR-CAS SYSTEMS; DROSOPHILA; GENE TARGETING; MUTAGENESIS; ORGAN SPECIFICITY;

EID: 84910619646     PISSN: None     EISSN: 21601836     Source Type: Journal    
DOI: 10.1534/g3.114.014159     Document Type: Article

References (32)

1. Bassett, A. R., C. Tibbit, C. P. Ponting, and J. L. Liu, 2013 Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system. Cell Reports 4: 220-228.
2. Beall, E. L., M. B. Mahoney, and D. C. Rio, 2002 Identification and analysis of a hyperactive mutant form of Drosophila P-element transposase. Genetics 162: 217-227.
3. Bischof, J., R. K. Maeda, M. Hediger, F. Karch, and K. Basler, 2007 An optimized transgenesis system for Drosophila using germ-line-specific fC31 integrases. Proc. Natl. Acad. Sci. USA 104: 3312-3317.
4. Blower, M. D., and G. H. Karpen, 2001 The role of Drosophila CID in kinetochore formation, cell-cycle progression and heterochromatin interactions. Nat. Cell Biol. 3: 730-739.
5. Blower, M. D., T. Daigle, T. Kaufman, and G. H. Karpen, 2006 Drosophila CENP-A mutations cause a BubR1-dependent early mitotic delay without normal localization of kinetochore components. PLoS Genet. 2: e110.
6. Carvalho, G. B., W. J. William, and S. Benzer, 2009 Non-lethal genotyping of single Drosophila. Biotechniques 46: 312.
7. Chen, D., and D. M. McKearin, 2003 A discrete transcriptional silencer in the bam gene determines asymmetric division of the Drosophila germline stem cell. Development 130: 1159-1170.
8. Choi, C. M., S. Vilain, M. Langen, S. Van Kelst, N. De Geest et al. 2009 Conditional mutagenesis in Drosophila. Science 324: 54.
9. Dietzl, G., D. Chen, F. Schnorrer, K. C. Su, Y. Barinova et al. 2007 A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448: 151-156.
10. Doren, M. V., A. L. Williamson, and R. Lehmann, 1998 Regulation of zygotic gene expression in Drosophila primordial germ cells. Curr. Biol. 8: 243-246.
11. Fuyama, Y., 1986 Genetics of parthenogenesis in Drosophila melanogaster. I. The modes of diploidization in the gynogenesis induced by a malesterile mutant, ms(3)K81. Genetics 112: 237-248.
12. Golic, K. G., 2013 RNA-guided nucleases: a new era for engineering the genomes of model and nonmodel organisms. Genetics 195: 303-308.
13. Gratz, S. J., A. M. Cummings, J. N. Nguyen, D. C. Hamm, L. K. Donohue et al. 2013 Genome engineering of Drosophila with the CRISPR RNAguided Cas9 nuclease. Genetics 194: 1029-1035.
14. Gratz, S. J., F. P. Ukken, C. D. Rubinstein, G. Thiede, L. K. Donohue et al. 2014 Highly specific and efficient CRISPR/Cas9-catalyzed homologydirected repair in Drosophila. Genetics 194: 961-971.
15. Gustafson, K., and G. Boulianne, 1996 Distinct expression patterns detected within individual tissues by the GAL4 enhancer trap technique. Genome 39: 174-182.
16. Johnston, L. A., and B. A. Edgar, 1998 Wingless and Notch regulate cellcycle arrest in the developing Drosophila wing. Nature 394: 82-84.
17. Kondo, S., and R. Ueda, 2013 Highly improved gene targeting by germlinespecific Cas9 expression in Drosophila. Genetics 915: 715-721.
18. Liu, P., L. Long, K. Xiong, B. Yu, N. Chang et al. 2014 Heritable/conditional genome editing in C. elegans using a CRISPR-Cas9 feeding system. Cell Res. 24: 886-889.
19. Ma, Y., X. Zhang, B. Shen, Y. Lu, W. Chen et al. 2013 Generating rats with conditional alleles using CRISPR/Cas9. Cell Res. 24: 122-125.
20. McKearin, D. M., and A. C. Spradling, 1990 bag-of-marbles: a Drosophila gene required to initiate both male and female gametogenesis. Genes Dev. 4: 2242-2251.
21. Ni, J.-Q., M. Markstein, R. Binari, B. Pfeiffer, L.-P. Liu et al. 2008 Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Nat. Methods 5: 49-51.
22. Ni, J. Q., L. P. Liu, R. Binari, R. Hardy, H. S. Shim et al. 2009 A Drosophila resource of transgenic RNAi lines for neurogenetics. Genetics 182: 1089-1100.
23. Ni, J.-Q., R. Zhou, B. Czech, L.-P. Liu, L. Holderbaum et al. 2011 A genome-scale shRNA resource for transgenic RNAi in Drosophila. Nat. Methods 8: 405-407.
24. Port, F., H. M. Chen, T. Lee, and S. L. Bullock, 2014 Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc. Natl. Acad. Sci. USA 111: E2967-E2976.
25. Presente, A., S. Shaw, J. S. Nye, and A. J. Andres, 2002 Transgene-mediated RNA interference defines a novel role for notch in chemosensory startle behavior. Genesis 34: 165-169.
26. Raychaudhuri, N., R. Dubruille, G. A. Orsi, H. C. Bagheri, B. Loppin et al. 2012 Transgenerational propagation and quantitative maintenance of paternal centromeres depends on Cid/Cenp-A presence in Drosophila sperm. PLoS Biol. 10: e1001434-e1001434.
27. Ren, X., J. Sun, B. E. Housden, Y. Hu, C. Roesel et al. 2013 Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9. Proc. Natl. Acad. Sci. USA 110: 19012-19017.
28. Rørth, P., 1998 Gal4 in the Drosophila female germline. Mech. Dev. 78: 113-118.
29. Thibault, S. T., M. A. Singer, W. Y. Miyazaki, B. Milash, N. A. Dompe et al. 2004 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. Nat. Genet. 36: 283-287.
30. Xue, Z., M. Ren, M. Wu, J. Dai, Y. S. Rong et al. 2014 Efficient gene knockout and knock-in with transgenic Cas9 in Drosophila. G3 (Bethesda) 4: 925-929.
31. Yang, H., H. Wang, C. S. Shivalila, A. W. Cheng, L. Shi et al. 2013 One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell 154: 1370-1379.
32. Yu, Z., M. Ren, Z. Wang, B. Zhang, Y. S. Rong et al. 2013 Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila. Genetics 195: 289-291.