Brain microvascular endothelial cells resist elongation due to curvature and shear stress

Scientific Reports

Volumn 4, Issue , 2014, Pages

  Ye, Mao ^a   Sanchez, Henry M ^a,b   Hultz, Margot ^a,b   Yang, Zhen ^b   Bogorad, Max ^a,b   Wong, Andrew D ^a,b   Searson, Peter C ^a,b  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b Whiting School of Engineering   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BLOOD BRAIN BARRIER; BRAIN; CELL CULTURE; CYTOLOGY; ENDOTHELIUM CELL; HUMAN; METABOLISM; MICROVASCULATURE; PHYSIOLOGICAL STRESS; TIGHT JUNCTION; TRANSPORT AT THE CELLULAR LEVEL; VASCULAR ENDOTHELIUM; VASCULARIZATION;

BIOLOGICAL TRANSPORT; BLOOD-BRAIN BARRIER; BRAIN; CELLS, CULTURED; ENDOTHELIAL CELLS; ENDOTHELIUM, VASCULAR; HUMANS; MICROVESSELS; STRESS, PHYSIOLOGICAL; TIGHT JUNCTIONS;

EID: 84910155854     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep04681     Document Type: Article

References (32)

1. Aird, W. C. Spatial and temporal dynamics of the endothelium. J. Thromb. Haemost. 3, 1392 (2005).
2. Abbott, N. J., Ronnback, L. & Hansson, E. Astrocyte-endothelial interactions at the blood-brain barrier. Nat. Rev. Neurosci. 7, 41 (2006).
3. Daneman, R. The blood-brain barrier in health and disease. Ann. Neurol. 72, 648 (2012).
4. Wong, A. D. et al. The blood-brain barrier: an engineering perspective. Front. Neuroeng. 6, 7 (2013).
5. Reese, T. S. & Karnovsky, M. J. Fine structural localization of a blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34, 207 (1967).
6. Brightman, M. W. Morphology of Blood-Brain Interfaces. Exp. Eye Res. 25, 1 (1977).
7. Curtis, A. S. G. & Varde, M. Control of cell behavior: topological factors. J. Natl. Cancer Inst. 33, 15 (1964).
8. Dunn, G. A. & Heath, J. P. A new hypothesis of contact guidance in tissue cells. Exp. Cell Res. 101, 1 (1976).
9. Fisher, P. E.& Tickle, C. Differences in alignment of normal and transformed cells on glass fibres. Exp. Cell Res. 131, 407 (1981).
10. Rovensky, Y. & Samoilov, V. I. Morphogenetic response of cultured normal and transformed fibroblasts, and epitheliocytes, to a cylindrical substratum surface. Possible role for the actin filament bundle pattern. J. Cell Sci. 107, 1255 (1994).
11. Svitkina, T. M., Rovensky, Y. A., Bershadsky, A. D. & Vasiliev, J. M. Transverse pattern of microfilament bundles induced in epitheliocytes by cylindrical substrata. J. Cell Sci. 108, 735 (1995).
12. Levina, E. M., Domnina, L. V., Rovensky, Y. A. & Vasiliev, J. M. Cylindrical substratum induces different patterns of actin microfilament bundles in nontransformed and in RAS-transformed epitheliocytes. Exp. Cell Res. 229, 159 (1996).
13. Hahn, C. & Schwartz, M. A. Mechanotransduction in vascular physiology and atherogenesis. Nat. Rev. Mol. Cell Biol. 10, 53 (2009).
14. Chien, S. Mechanotransduction and endothelial cell homeostasis: the wisdom of the cell. Am. J. Physiol. Heart Circ. Physiol. 292, H1209 (2007).
15. Johnson, B. D., Mather, K. J. & Wallace, J. P. Mechanotransduction of shear in the endothelium: basic studies and clinical implications. Vasc. Med. 16, 365 (2011).
16. Conway, D. & Schwartz, M. A. Lessons from the endothelial junctional mechanosensory complex. F1000 Biol. Rep. 4, 1 (2012).
17. Aird, W. C. Phenotypic heterogeneity of the endothelium: II. Representative vascular beds. Circ. Res. 100, 174 (2007).
18. Aird, W. C. Phenotypic heterogeneity of the endothelium: I. Structure, function, and mechanisms. Circ. Res. 100, 158 (2007).
19. Nerem, R.M., Levesque, M. J. & Cornhill, J. F. Vascular endothelial morphology as an indicator of the pattern of blood-flow. J. Biomech. Eng. Trans. ASME 103, 172 (1981).
20. Reidy, M. A. & Langille, B. L. The effect of local blood flow patterns on endothelial cell morphology. Exp. Mol. Pathol. 32, 276 (1980).
21. Malek, A. M. & Izumo, S. Mechanism of endothelial cell shape change and cytoskeletal remodeling in response to fluid shear stress. J. Cell Sci. 109, 713 (1996).
22. Levesque, M. J. & Nerem, R. M. The elongation and orientation of cultured endothelial-cells in response to shear-stress. J. Biomech. Eng. Trans. ASME 107, 341 (1985).
23. Eskin, S. G., Ives, C. L., Mcintire, L. V. & Navarro, L. T. Response of cultured endothelial-cells to steady flow. Microvasc. Res. 28, 87 (1984).
24. Davies, P. F. Flow-Mediated Endothelial Mechanotransduction. Physiol. Rev. 75, 519 (1995).
25. Simmers, M. B., Pryor, A. W. & Blackman, B. R. Arterial shear stress regulates endothelial cell-directed migration, polarity, and morphology in confluent monolayers. Am. J. Physiol. Heart Circ. Physiol. 293, H1937 (2007).
26. Levesque, M. J., Liepsch, D., Moravec, S. & Nerem, R. M. Correlation of endothelial-cell shape and wall shear-stress in a stenosed dog aorta. Arteriosclerosis 6, 220 (1986).
27. Silkworth, J. B. & Stehbens, W. E. Shape of endothelial cells in en-face preparations of rabbit blood-vessels. Angiology 26, 474 (1975).
28. Franke, R. P. et al. Induction of human vascular endothelial stress fibres by fluid shear stress. Nature 307, 648 (1984).
29. Abbott, N. J., Patabendige, A. A., Dolman, D. E., Yusof, S. R. & Begley, D. J. Structure and function of the blood-brain barrier. Neurobiol. Dis. 37, 13 (2010).
30. Nizet, V. et al. Invasion of brain microvascular endothelial cells by group B streptococci. Infect. Immun. 65, 5074 (1997).
31. Stins, M. F., Prasadarao, N. V., Zhou, J., Arditi, M. & Kim, K. S. Bovine brain microvascular endothelial cells transfected with SV40-large T antigen: development of an immortalized cell line to study pathophysiology of CNS disease. In Vitro Cell. Dev. Biol. Anim. 33, 243 (1997).
32. Eigenmann, D. E. et al. Comparative study of four immortalized human brain capillary endothelial cell lines, hCMEC/D3, hBMEC, TY10, and BB19, and optimization of culture conditions, for an in vitro blood-brain barrier model for drug permeability studies. Fluids Barriers CNS 10, 33 (2013).