Simultaneous purification and refolding of proteins by affinity precipitation and macro (affinity ligand)- facilitated three phase partitioning (MLFTPP)

Methods in Molecular Biology

Volumn 1129, Issue , 2014, Pages 111-123

  Roy, Ipsita ^a   Mondal, Kalyani ^a   Gupta, Munishwar N ^a  

^a NONE

Author keywords

Affinity precipitation; Alginate; Cellulase; Chitosan; Eudragit; Macro (affinity ligand); Pectinase; Protein purification; Smart polymers; Three phase partitioning; Xylanase

Indexed keywords

AMMONIUM SULFATE; AMYLASE; BUTANOL; CELLULASE; EUDRAGIT; GLUCAN 1,4 ALPHA GLUCOSIDASE; POLYGALACTURONASE; POLYMER; PULLULANASE; XYLAN ENDO 1,3 BETA XYLOSIDASE; LIGAND; PROTEIN;

AFFINITY PRECIPITATION; ARTICLE; ASPERGILLUS NIGER; BACILLUS; BACILLUS ACIDOPULLULYTICUS; FLUORESCENCE; MACRO AFFINITY LIGAND FACILITATED THREE PHASE PARTITIONING; MICROWAVE RADIATION; NONHUMAN; PHASE PARTITIONING; PRECIPITATION; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN REFOLDING; AFFINITY CHROMATOGRAPHY; CHEMISTRY; ISOLATION AND PURIFICATION; METHODOLOGY; PROTEIN FOLDING;

CHROMATOGRAPHY, AFFINITY; LIGANDS; PROTEIN FOLDING; PROTEINS;

EID: 84910018031     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-977-2_11     Document Type: Article

References (40)

1. Przybycien TM, Pujar NS, Steele LM (2004) Alternative bioseparation operations: life beyond packed-bed chromatography. Curr Opin Biotechnol 15: 469-478.
2. Mondal K, Gupta MN, Roy I (2006) Affinitybased strategies for protein purification. Anal Chem 78: 3499-3504.
3. Mattiasson B (1988) Bioconversions in aqueous 2-phase systems-an alternative to conventional immobilization. Methods Enzymol 137: 657-667.
4. Kula MR (1990) Trends and future prospects of aqueous two-phase extraction. Bioseparation 1: 181-189.
5. Teotia S, Gupta MN (2001) Reversibly soluble macroaffinity ligand in aqueous two-phase separation of enzymes. J Chromatogr A 923: 275-280.
6. Pecs M, Eggert M, Schugerl K (1991) Affinity precipitation of extracellular microbial enzymes. J Biotechnol 21: 137-142.
7. Gupta MN, Mattiasson B (1994) Affinity precipitation. In: Street G (ed) Highly selective separations in biotechnology. Chapman and Hall, London, pp 7-33.
8. Gupta MN (2002) Methods in affinity based separation of proteins/enzymes. In: Gupta MN (ed) Birkhauser Verlag, Basel.
9. Roy I, Gupta MN (2003) Smart polymeric materials: emerging biochemical applications. Chem Biol 10: 1161-1171.
10. Roy I, Gupta MN (2000) Current trends in affinity-based separations of proteins/enzymes. Curr Sci 78: 587-591.
11. Gupta MN, Kapoor M, Majumder AB, Singh V (2011) Isozymes, moonlighting proteins and promiscous enzymes. Curr Sci 100: 1152-1162.
12. Vijayalakshmi MA (1989) Pseudo-biospecific ligand affinity chromatography. Tibtech 7: 71-76.
13. Grigoryan G, Reinke AW, Keating AE (2009) Design of protein-interaction specificity gives selective bZIP-binding peptides. Nature 458: 859-865.
14. Douglas SM, Bachelet I, Church GM (2012) A logic-gated nanorobot for targeted transport of molecular payloads. Science 335: 831-834.
15. Gupta MN, Dong G, Mattiasson B (1993) Purification of endo-polygalacturonase by affinity precipitation using alginate. Biotechnol Appl Biochem 18: 321-327.
16. Sharma A, Sharma S, Gupta MN (2000) Purification of wheat germ amylase by precipitation. Protein Expr Purif 18: 111-114.
17. Sharma S, Gupta MN (2001) Alginate as a macroaffinity ligand and an additive for enhanced activity and thermostability of lipases. Biotechnol Appl Biochem 33: 161-165.
18. Teotia S, Khare SK, Gupta MN (2001) An effi-cient purification process for sweet potato beta-amylase by affinity precipitation with alginate. Enzyme Microb Technol 28: 792-795.
19. Teotia S, Lata R, Khare SK, Gupta MN (2001) One-step purification of glucoamylase by affinity precipitation with alginate. J Mol Recognit 14: 295-299.
20. Dennison C, Lovrien R (1997) Three phase partitioning: concentration and purification of proteins. Protein Expr Purif 11: 149-161.
21. Sharma A, Sharma S, Gupta MN (2000) Purification of alkaline phosphatase from chicken intestine by three-phase partitioning and use of phenyl-Sepharose 6B in the batch mode. Bioseparation 9: 155-161.
22. Sharma A, Gupta MN (2001) Three phase partitioning as a large-scale separation method for purification of a wheat germ bifunctional protease/amylase inhibitor. Process Biochem 37: 193-196.
23. Sharma A, Gupta MN (2001) Purification of pectinases by three-phase partitioning. Biotechnol Lett 23: 1625-1627.
24. Sharma S, Gupta MN (2001) Purification of phospholipase D from Dacus carota by threephase partitioning and its characterization. Protein Expr Purif 21: 310-316.
25. Roy I, Gupta MN (2002) Three-phase affinity partitioning of proteins. Anal Biochem 300: 11-14.
26. Jain S, Singh R, Gupta MN (2004) Purification of recombinant green fluorescent protein by three-phase partitioning. J Chromatogr A 1035: 83-86.
27. Sharma A, Gupta MN (2002) Macroaffinity ligand-facilitated three-phase partitioning (MLFTPP) for purification of xylanase. Biotechnol Bioeng 80: 228-232.
28. Sharma A, Roy I, Gupta MN (2004) Affinity precipitation and macroaffinity ligand facilitated three-phase partitioning for refolding and simultaneous purification of urea-denatured pectinase. Biotechnol Prog 20: 1255-1258.
29. Mondal K, Bohidar HB, Roy RP, Gupta MN (2006) Alginate-chaperoned facile refolding of Chromobacterium viscosum lipase. Biochim Biophys Acta 1764: 877-886.
30. Gautam S, Dubey P, Singh P, Kesavardhana S, Vardarajan R, Gupta MN (2012) Smart polymer mediated purification and recovery of active proteins from inclusion bodies. J Chromatogr A. doi: 10. 1016/j. chroma. 2012. 02. 048.
31. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-428.
32. Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem 59: 257-268.
33. Bailey M, Poutanen K (1989) Production of xylanolytic enzymes by strains of Aspergillus. Appl Microbiol Biotechnol 30: 5-10.
34. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 72: 248-254.
35. Roy I, Mondal K, Sharma A, Gupta MN (2005) Simultaneous refolding/purification of xylanase with a microwave treated smart polymer. Biochim Biophys Acta 1747: 179-187.
36. Sharma A, Gupta MN (2002) Three phase partitioning of carbohydrate polymers: separation and purification of alginates. Carbohydr Polym 48: 391-395.
37. Sharma A, Mondal K, Gupta MN (2003) Some studies on characterization of three phase partitioned chitosan. Carbohydr Polym 52: 433-438.
38. Mondal K, Sharma A, Gupta MN (2003) Macroaffinity ligand-facilitated three-phase partitioning for purification of glucoamylase and pullulanase using alginate. Protein Expr Purif 28: 190-195.
39. Mondal K, Sharma A, Lata L, Gupta MN (2003) Macroaffinity ligand-facilitated threephase partitioning (MLFTPP) of alphaamylases using a modified alginate. Biotechnol Prog 19: 493-494.
40. Sharma A, Mondal K, Gupta MN (2003) Separation of enzymes by sequential macroaffinity ligand-facilitated three-phase partitioning. J Chromatogr A 995: 127-134.