Multiphoton imaging of tumor biomarkers with conjugates of single-domain antibodies and quantum dots

Nanomedicine: Nanotechnology, Biology, and Medicine

Volumn 10, Issue 8, 2014, Pages 1701-1709

  Hafian, Hilal ^a   Sukhanova, Alyona ^a,b   Turini, Marc ^c,d,e   Chames, Patrick ^c,d,e   Baty, Daniel ^c,d,e   Pluot, Michel ^a   Cohen, Jacques H M ^a   Nabiev, Igor ^a,b   Millot, Jean Marc ^a  

^a UNIVERSITÉ DE REIMS CHAMPAGNE ARDENNE   (France)

^b NATIONAL RESEARCH NUCLEAR UNIVERSITY MEPHI   (Russian Federation)

^c AIX MARSEILLE UNIVERSITY   (France)

^d INSTITUT PAOLI CALMETTES   (France)

^e AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Diagnostic nanoprobes; Immunohistochemistry; Quantum dots; Single domain antibodies; Two photon imaging

Indexed keywords

ANTIBODIES; ANTIGENS; CADMIUM COMPOUNDS; DIAGNOSIS; GRAPHENE QUANTUM DOTS; II-VI SEMICONDUCTORS; NANOCRYSTALS; NANOPROBES; PHOTONS; PROBES; SELENIUM COMPOUNDS; TUMORS; TWO PHOTON PROCESSES;

CARCINOEMBRYONIC ANTIGEN; IMMUNOHISTOCHEMISTRY; MULTI-PHOTON MICROSCOPY; ONE PHOTON EXCITATIONS; SINGLE-DOMAIN ANTIBODIES; TWO PHOTON IMAGING; TWO-PHOTON ABSORPTION CROSS SECTION; TWO-PHOTON EXCITATIONS;

SEMICONDUCTOR QUANTUM DOTS;

ANTIBODY CONJUGATE; CADMIUM; CARCINOEMBRYONIC ANTIGEN; DYE; IMMUNOGLOBULIN G; NANOCRYSTAL; QUANTUM DOT; SELENIUM; STREPTAVIDIN; TUMOR MARKER; ZINC SULFIDE; NANOBODY;

ANTIGEN DETECTION; ANTIGEN EXPRESSION; ARTICLE; ARTIODACTYLA; AUTOFLUORESCENCE; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; MOLECULAR RECOGNITION; MULTIPHOTON MICROSCOPY; NANOPROBE; ANIMAL; CHEMISTRY; DIAGNOSTIC IMAGING; IN VITRO STUDY; PROCEDURES;

ANIMALS; DIAGNOSTIC IMAGING; IN VITRO TECHNIQUES; QUANTUM DOTS; SINGLE-DOMAIN ANTIBODIES; TUMOR MARKERS, BIOLOGICAL;

EID: 84908575363     PISSN: 15499634     EISSN: 15499642     Source Type: Journal    
DOI: 10.1016/j.nano.2014.05.014     Document Type: Article

References (35)

1. Denk W., Strickler J.H., Webb W.W. Two-photon laser scanning fluorescence microscopy. Science 1990, 248:73-76.
2. Larson D.R., Warren R.Z., William R.M., Clark S.W., Bruchez M.P., Wise F.W., et al. Water soluble quantum dots for multiphoton fluorescence imaging in vivo. Science 2003, 300:1434-1436.
3. Xing Y., Rao J. Quantum dot bioconjugates for in vitro diagnostics & in vivo imaging. Cancer Biomark 2008, 4:307-319.
4. Samokhvalov P., Artemyev M., Nabiev I. Basic principles and current trends in colloidal synthesis of highly luminescent semiconductor nanocrystals. Chem Eur J 2013, 19:1534-1546.
5. Jańczewski D., Tomczak N., Han M.-Y., Vancso G.J. Synthesis of functionalized amphiphilic polymers for coating quantum dots. Nat Protoc 2011, 6:1546-1553.
6. Sukhanova A., Even-Desrumeaux K., Chames P., Baty D., Artemyev M., Oleinikov V., et al. Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots. Nat Protoc Protoc Exch 2012, [http://www.nature.com/protocolexchange/protocols/2463]. 10.1038/protex.2012.042.
7. Pu S.C., Yang M.J., Hsu C.C., Lai C.W., Hsieh C.C., Lin S.H., et al. The empirical correlation between size and two-photon absorption cross section of CdSe and CdTe quantum dots. Small 2006, 2:1308-1313.
8. Saerens D., Kinne J., Bosmans E., Wernery U., Muyldermans S., Conrath K. Single domain antibodies derived from dromedary lymph node and peripheral blood lymphocytes sensing conformational variants of prostate-specific antigen. J Biol Chem 2004, 279:51965-51972.
9. Perruchini C., Pecorari F., Bourgeois J.P., Duyckaerts C., Rougeon F., Lafaye P. Llama VHH antibody fragments against GFAP: better diffusion in fixed tissues than classical monoclonal antibodies. Acta Neuropathol 2009, 118:685-695.
10. Dumoulin M., Conrath K., Van Meirhaeghe A., Meersman F., Heremans K., Frenken L.G.J., et al. Single-domain antibody fragments with high conformational stability. Protein Sci 2002, 11:500-515.
11. Olichon A., Schweizer D., Muyldermans S., de Marco A. Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains. BMC Biotechnol 2007, 7:7-14.
12. Sukhanova A., Even-Desruleaux K., Kisserli A., Tabary T., Reveil B., Millot J.M., et al. Oriented conjugates of single domain antibodies and quantum dots: toward a new generation of ultrasmall diagnostic nanoprobes. Nanomedecine 2012, 8:516-525.
13. Behar G., Chames P., Teulon I., Cornillon A., Alshoukr F., Roquet F., et al. Llama single domain antibodies directed against non-conventional epitopes of tumor-associated carcinoembryonic antigen (CEA) absent from non-specific cross-reacting antigen (NCA). FEBS J 2009, 276:3881-3893.
14. Even-Desrumeaux K., Baty D., Chames P. Strong and oriented immobilization of single domain antibodies from crude bacterial lysates for high-throughput compatible cost-effective antibody array generation. Mol Biosyst 2010, 6:2241-2248.
15. Even-Desrumeaux K., Fourquet P., Secq V., Baty D., Chames P. Single-domain antibodies: a versatile and rich source of binders for breast cancer diagnostic approaches. Mol Biosyst 2012, 8:2385-2394.
16. Annunziato M.E., Patel U.S., Ranade M., Palumbo P.S. P-maleimidophenyl isocyanate: a novel heterobifunctional linker for hydroxyl to thiol coupling. Bioconjug Chem 1993, 4:212-218.
17. Rozan C., Cornillon A., Pétiard C., Chartier M., Behar G., Boix C., et al. Single domain antibody-based and linker-free bispecific antibodies targeting FcγRIII induce potent anti-tumor activity without recruiting regulatory T cells. Mol Cancer Ther 2013, 12:1481-1491.
18. Yu W.W., Qu L., Guo W., Peng X. Experimental determination of the extinction coefficient of CdTe, CdSe, and CdS nanocrystals. Chem Mater 2003, 15:2854-2860.
19. Jasieniak J., Smith L., van Embden J., Mulvaney P. Re-examination of the size-sependent absorption properties of CdSe quantum dots. J Phys Chem C 2009, 113:19468-19474.
20. Doose S., Tsay J.M., Pinaud F., Weiss S. Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy. Anal Chem 2005, 77:2235-2242.
21. Nap M., Hammarström M.L., Börmer O., Hammarström S., Wagener C., Handt S., et al. Specificity and affinity of monoclonal antibodies against carcinoembryonic antigen. Cancer Res 1992, 8:2329-2339.
22. Blanton S.A., Dehestani A., Lin P.C., Guyot-Sionnest P. Photoluminescence of single semiconductor nanocrystallites by two-photon excitation microscopy. Chem Phys Lett 1994, 229:317-322.
23. Gold P., Goldenberg N.A. The carcinoembryonic antigen (CEA): past, present, and future. MJM 1997, 3:46-66.
24. Hammarstrom S. The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin Cancer Biol 1999, 9:67-81.
25. Puertas S., Moros M., Fernández-Pacheco R., Ibarra M.R., Grazu V., de la Fuente J.M. Designing novel nano-immunoassays: antibody orientation vs sensitivity. J Phys D Appl Phys 2010, 43:474012.
26. Puertas S., Batalla P., Moros M., Polo E., Pino P., Guisan J.M. Taking advantage of unspecific interactions to produce highly active magnetic nanoparticle-antibody conjugates. ACS Nano 2011, 5:4521-4528.
27. Montenegro J.-M., Grazu V., Sukhanova A., Agarwal S., de la Fuente J.M., Nabiev I., et al. Controlled antibody/(bio-) conjugation of inorganic nanoparticles for targeted delivery. Adv Drug Del Rev 2013, 65:677-688.
28. Xu C., Warren R.Z., Shear J.B., William R.M., Webb W.W. Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy. Proc Natl Acad Sci U S A 1996, 93:10763-10768.
29. Sukhanova A., Venteo L., Devy J., Artemyev M., Oleinikov V., Pluot M., et al. Highly stable fluorescent nanocrystals as a novel class of labels for immunohistochemical analysis of paraffin-embedded tissue sections. Lab Invest 2002, 82:1259-1262.
30. Sukhanova A., Devy J., Venteo L., Kaplan H., Artemyev M., Oleinikov V., et al. Biocompatible fluorescent nanocrystals for immunolabeling of membrane proteins and cells. Anal Biochem 2004, 324:60-67.
31. Ness J.M., Akhtar R.S., Latham C.B., Roth K.A. Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection. J Histochem Cytochem 2003, 51:981-987.
32. Zahavy E., Freeman E., Lustig S., Keysary A., Yitzhaki S. Double labeling and simultaneous detection of B- and T-cells using fluorescent nano-crystal (q-dots) in paraffin-embedded tissues. J Fluoresc 2005, 15:661-665.
33. Liu J., Lau S.K., Varma V.A., Moffitt R.A., Caldwell M., Liu T., et al. Molecular mapping of tumor heterogeneity on clinical tissue specimens with multiplexed quantum dots. ACS Nano 2010, 4:2755-2765.
34. Byers R.J., Vizio D.D., O'Connell F., Tholouli E., Levenson R.M., Gossard K., et al. Semi automated multiplexed quantumdDot-based in situ hybridization and spectral deconvolution. J Mol Diagn 2007, 9:20-29.
35. Mahmoud W., Rousserie G., Reveil B., Tabary T., Millot J.M., Artemyev M., et al. Advanced procedures for labeling of antibodies with quantum dots. Anal Biochem 2011, 416:180-185.