Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection

Journal of Histochemistry and Cytochemistry

Volumn 51, Issue 8, 2003, Pages 981-987

  Ness, Jayne M ^a,b   Akhtar, Rizwan S ^b   Latham, Cecelia B ^b   Roth, Kevin A ^b  

^a UAB   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

Immunohistochemistry; Nanocrystals; Photobleaching; Tyramide signal amplification

Indexed keywords

HORSERADISH PEROXIDASE; ISOPROPAMIDE IODIDE; STREPTAVIDIN;

ANIMAL TISSUE; ANTIGEN BINDING; ANTIGEN DETECTION; ANTIGEN RETRIEVAL; ARTICLE; BLEACHING; BRAIN; CONTROLLED STUDY; CRYSTAL; FROZEN SECTION; ILLUMINATION; IMMUNODETECTION; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; INCUBATION TIME; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALIA; ARMORACIA RUSTICANA;

EID: 0043246612     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540305100801     Document Type: Article

References (15)

1. Bobrow MN, Harris TD, Shaughnessy KJ, Litt GJ (1989) Catalyzed reporter deposition, a novel method of signal amplification: application to membrane immunoassays. J Immunol 125:279-285
2. Bobrow MN, Litt GJ, Shaughnessy KJ, Mayer PC, Conlon J (1992) The use of catalyzed reporter deposition as a means of signal amplification in a variety of formats. J Immunol Methods 150:145-149
3. Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281:2013-2016
4. Chan WC, Maxwell DJ, Gao X, Bailey R, Han M, Nie S (2002) Luminescent quantum dots for multiplexed biological detection and imaging. Curr Opin Biotech 13:40-46
5. Chan WC, Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016-2018
6. Mitchell P (2001) Turning the spotlight on cellular imaging. Nature Biotech 19:1013-1017
7. Ono M, Murakami T, Kudo A, Isshiki M, Sawada H, Segawa A (2001) Quantitative comparison of anti-fading mounting media for confocal laser scanning microscopy. J Histochem Cytochem 49:305-311
8. Papandrikopoulou A, Doll T, Tucker RP, Garner CC, Matus A (1989) Embryonic MAP2 lacks the cross-linking sidearm sequences and dendritic targeting signal of adult MAP2. Nature 340:650-652
9. Shindler KS, Roth KA (1996) Double immunofluorescent staining using two unconjugated primary antisera raised in the same species. J Histochem Cytochem 44:1331-1335
10. Sutherland AJ (2002) Quantum dots as luminescent probes in biological systems. Curr Opin Solid State Mater Sci 6:365-370
11. van Gijlswijk RP, Zijlmans HJ, Weigant J, Bobrow MN, Erickson TJ, Adler KE, Tanke HJ, et al. (1997) Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem 45:375-382
12. Watson A, Wu X, Bruchez M (2003) Lighting up cells with quantum dots. BioTechniques 34:296-303
13. Wiedenmann B, Franke WW (1985) Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 41:1017-1028
14. Wu X (2003) Detecting nuclear antigens using Qdot™ streptavidin conjugates. Quantum Dot Vis 1:10-13
15. Wu X, Liu H, Liu J, Haley KN, Treadway JA, Larson JP, Ge N, et al. (2003) Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots. Nature Biotech 21:41-46