Optimization and revision of the production process of the Necator americanus glutathione s-transferase 1 (Na-GST-1), the lead hookworm vaccine recombinant protein candidate

Human Vaccines and Immunotherapeutics

Volumn 10, Issue 7, 2014, Pages 1914-1925

  Curti, Elena ^a,b   Seid, Christopher A ^a,b   Hudspeth, Elissa ^a,b   Center, Lori ^a,b   Rezende, Wanderson ^a,b   Pollet, Jeroen ^a,b   Kwityn, Cliff ^a,d   Hammond, Molly ^a,b   Matsunami, Rise K ^c   Engler, David A ^c   Hotez, Peter J ^a,b   Bottazzi, Maria Elena ^a,b  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b TEXAS CHILDREN S HOSPITAL   (United States)

^c HOUSTON METHODIST RESEARCH INSTITUTE   (United States)

^d Beckman Coulter Inc   (United States)

Author keywords

Fermentation; Hookworm; Necator americanus; Purification; Vaccine

Indexed keywords

ALUMINUM HYDROXIDE; GLUTATHIONE TRANSFERASE; GLUTATHIONE TRANSFERASE 1; UNCLASSIFIED DRUG; CULTURE MEDIUM; PARASITE ANTIGEN; RECOMBINANT PROTEIN; RECOMBINANT VACCINE;

ANTIGEN EXPRESSION; ARTICLE; CONTROLLED STUDY; DISULFIDE BOND; DRUG PURITY; DRUG STORAGE; FERMENTATION OPTIMIZATION; FREEZE THAWING; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NECATOR AMERICANUS; PROCESS OPTIMIZATION; PROTEIN PURIFICATION; PROTEIN STABILITY; VACCINE PRODUCTION; ANIMAL; BIOTECHNOLOGY; CHEMISTRY; CULTURE MEDIUM; DRUG STABILITY; ENZYMOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; HOOKWORM INFECTIONS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; MEDICINAL CHEMISTRY; METABOLISM; PHARMACEUTICS; PICHIA; PROCEDURES; TRANSCRIPTION INITIATION;

ANIMALS; ANTIGENS, HELMINTH; BIOTECHNOLOGY; CHEMISTRY, PHARMACEUTICAL; CHROMATOGRAPHY, LIQUID; CULTURE MEDIA; DRUG STABILITY; GLUTATHIONE TRANSFERASE; HOOKWORM INFECTIONS; HUMANS; NECATOR AMERICANUS; PICHIA; RECOMBINANT PROTEINS; TECHNOLOGY, PHARMACEUTICAL; TRANSCRIPTIONAL ACTIVATION; VACCINES, SYNTHETIC;

EID: 84908370786     PISSN: 21645515     EISSN: 2164554X     Source Type: Journal    
DOI: 10.4161/hv.28872     Document Type: Article

References (20)

1. Hotez P. Enlarging the "Audacious Goal": elimination of the world's high prevalence neglected tropical diseases. Vaccine 2011; 29(Suppl 4):D104-10; PMID:22188933; http://dx.doi.org/10.1016/j.vaccine.2011.06.024
2. Hotez PJ, Diemert D, Bacon KM, Beaumier C, Bethony JM, Bottazzi ME, Brooker S, Couto AR, Freire MdaS, Homma A, et al. The Human Hookworm Vaccine. Vaccine 2013; 31(Suppl 2):B227-32; PMID:23598487; http://dx.doi.org/10.1016/j.vaccine.2012.11.034
3. Goud GN, Deumic V, Gupta R, Brelsford J, Zhan B, Gillespie P, Plieskatt JL, Tsao EI, Hotez PJ, Bottazzi ME. Expression, purification, and molecular analysis of the Necator americanus glutathione S-transferase 1 (Na-GST-1): a production process developed for a lead candidate recombinant hookworm vaccine antigen. Protein Expr Purif 2012; 83:145-51; PMID:22503665; http://dx.doi.org/10.1016/j.pep.2012.03.013
4. Hotez PJ, Diemert D, Bacon KM, Beaumier C, Bethony JM, Bottazzi ME, Brooker S, Couto AR, Freire MdaS, Homma A, et al. The Human Hookworm Vaccine. Vaccine 2013; 31(Suppl 2):B227-32; PMID:23598487; http://dx.doi.org/10.1016/j.vaccine.2012.11.034
5. Kassebaum NJ, Jasrasaria R, Naghavi M, Wulf SK, Johns N, Lozano R, et al. A systematic analysis of global anemia burden from 1990 to 2010. Blood 2014;
6. Murray CJ, Vos T, Lozano R, Naghavi M, Flaxman AD, Michaud C, Ezzati M, Shibuya K, Salomon JA, Abdalla S, et al. Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 2012; 380:2197-223; PMID:23245608; http://dx.doi.org/10.1016/S0140-6736(12)61689-4
7. Vos T, Flaxman AD, Naghavi M, Lozano R, Michaud C, Ezzati M, Shibuya K, Salomon JA, Abdalla S, Aboyans V, et al. Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 2012; 380:2163-96; PMID:23245607; http://dx.doi.org/10.1016/S0140-6736(12)61729-2
8. Asojo OA, Homma K, Sedlacek M, Ngamelue M, Goud GN, Zhan B, Deumic V, Asojo O, Hotez PJ. X-ray structures of Na-GST-1 and Na-GST-2 two glutathione S-transferase from the human hookworm Necator americanus. BMC Struct Biol 2007; 7:42; PMID:17594497; http://dx.doi.org/10.1186/1472-6807-7-42
9. Zhan B, Liu S, Perally S, Xue J, Fujiwara R, Brophy P, Xiao S, Liu Y, Feng J, Williamson A, et al. Biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm Ancylostoma caninum. Infect Immun 2005; 73:6903-11; PMID:16177370; http://dx.doi.org/10.1128/ IAI.73.10.6903-6911.2005
10. Xiao S, Zhan B, Xue J, Goud GN, Loukas A, Liu Y, Williamson A, Liu S, Deumic V, Hotez P. The evaluation of recombinant hookworm antigens as vaccines in hamsters (Mesocricetus auratus) challenged with human hookworm, Necator americanus. Exp Parasitol 2008; 118:32-40; PMID:17645877; http://dx.doi.org/10.1016/j.exppara.2007.05.010
11. Zhan B, Perally S, Brophy PM, Xue J, Goud G, Liu S, Deumic V, de Oliveira LM, Bethony J, Bottazzi ME, et al. Molecular cloning, biochemical characterization, and partial protective immunity of the heme-binding glutathione S-transferases from the human hookworm Necator americanus. Infect Immun 2010; 78:1552-63; PMID:20145100; http://dx.doi.org/10.1128/ IAI.00848-09
12. Plieskatt JL, Rezende WC, Olsen CM, Trefethen JM, Joshi SB, Middaugh CR, Hotez PJ, Bottazzi ME. Advances in vaccines against neglected tropical diseases: enhancing physical stability of a recombinant hookworm vaccine through biophysical and formulation studies. Hum Vaccin Immunother 2012; 8:765-76; PMID:22495115; http://dx.doi.org/10.4161/hv.19726
13. WHO. Deworming for health and development. Report of the third global meeting of the partners for parasite control. World Health Organization, Geneva, Switzerland. 2005.
14. Hotez P. A handful of 'antipoverty' vaccines exist for neglected diseases, but the world's poorest billion people need more. Health Aff (Millwood) 2011; 30:1080-7; PMID:21653960; http://dx.doi.org/10.1377/ hlthaff.2011.0317
15. Celik E, Calik P, Oliver SG. Fed-batch methanol feeding strategy for recombinant protein production by Pichia pastoris in the presence of co-substrate sorbitol. Yeast 2009; 26:473-84; PMID:19575480; http://dx.doi.org/10.1002/yea.1679
16. Jungo C, Schenk J, Pasquier M, Marison IW, von Stockar U. A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris. J Biotechnol 2007; 131:57-66; PMID:17614154; http://dx.doi.org/10.1016/j.jbiotec.2007.05.019
17. Ramón R, Ferrer P, Valero F. Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris. J Biotechnol 2007; 130:39-46; PMID:17399833; http://dx.doi.org/10.1016/j.jbiotec.2007.02.025
18. Shen Y, Gu L, Zhang J, Chen J, Du G. [Effects of mixed carbon sources on glucose oxidase production by recombinant Pichia pastoris]. Sheng Wu Gong Cheng Xue Bao 2013; 29:927-36; PMID:24195359
19. Wang Z, Wang Y, Zhang D, Li J, Hua Z, Du G, Chen J. Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding with methanol in Pichia pastoris fermentation. Bioresour Technol 2010; 101:1318-23; PMID:19800218; http://dx.doi.org/10.1016/j.biortech.2009.09.025
20. Shevchenko A, Tomas H, Havlis J, Olsen JV, Mann M. In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat Protoc 2006; 1:2856-60; PMID:17406544; http://dx.doi.org/10.1038/nprot.2006.468