Single-cell cloning and expansion of human induced pluripotent stem cells by a microfluidic culture device

Biochemical and Biophysical Research Communications

Volumn 453, Issue 1, 2014, Pages 131-137

  Matsumura, Taku ^a   Tatsumi, Kazuya ^b   Noda, Yuichiro ^a   Nakanishi, Naoyuki ^a   Okonogi, Atsuhito ^a   Hirano, Kunio ^a   Li, Liu ^c   Osumi, Takashi ^a   Tada, Takashi ^d   Kotera, Hidetoshi ^b  

^a ARKRAY Healthcare Pvt Ltd   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c KYOTO UNIVERSITY   (Japan)

^d KYOTO UNIVERSITY   (Japan)

Author keywords

Culture conditions; Human induced pluripotent stem cells; Microenvironment; Microfluidic culture; Single cell cloning

Indexed keywords

ARTICLE; CELL CLONING; CELL CULTURE MONITORING; CELL GROWTH; CELL LEVEL; CELL PROLIFERATION; CELL TRACKING; CLONAL VARIATION; CULTURE MEDIUM; HUMAN; HUMAN CELL; IN VITRO STUDY; MICROFLUIDIC CULTURE DEVICE; MICROFLUIDICS; PLURIPOTENT STEM CELL; PLURIPOTENT STEM CELL LINE; SINGLE CELL ANALYSIS; STEM CELL CULTURE; STEM CELL EXPANSION; BIOLOGICAL MODEL; CELL CLONE; COMPUTER SIMULATION; CULTURE TECHNIQUE; CYTOLOGY; DEVICES; EQUIPMENT DESIGN; GENE EXPRESSION PROFILING; METABOLISM; MICROFLUIDIC ANALYSIS; STEM CELL NICHE; VALIDATION STUDY;

CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CLONE CELLS; COMPUTER SIMULATION; EQUIPMENT DESIGN; GENE EXPRESSION PROFILING; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MICROFLUIDIC ANALYTICAL TECHNIQUES; MODELS, BIOLOGICAL; STEM CELL NICHE;

EID: 84908348211     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2014.09.081     Document Type: Article

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