PCR-based detection of Toxoplasma gondii DNA in blood and ocular samples for diagnosis of ocular toxoplasmosis

Journal of Clinical Microbiology

Volumn 52, Issue 11, 2014, Pages 3987-3991

  Bourdin, C ^a   Busse, A ^a   Kouamou, E ^a   Touafek, F ^a   Bodaghi, B ^a   Le Hoang, P ^a   Mazier, D ^a,b   Paris, L ^a   Fekkar, A ^a,b  

^a PITIÉ SALPÊTRIÈRE HOSPITAL   (France)

^b France   (France)

Author keywords

[No Author keywords available]

Indexed keywords

PROTOZOAL DNA;

ADOLESCENT; ADULT; AGED; ARTICLE; BLOOD SAMPLING; CALCULATION; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DNA DETERMINATION; DNA EXTRACTION; DNA SEQUENCE; FEMALE; HUMAN; IMMUNOBLOTTING; IMMUNOCOMPROMISED PATIENT; MAJOR CLINICAL STUDY; MALE; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; TOXOPLASMOSIS; VERY ELDERLY; BLOOD; COMPARATIVE STUDY; EVALUATION STUDY; EYE; GENETICS; ISOLATION AND PURIFICATION; MIDDLE AGED; MOLECULAR DIAGNOSIS; PARASITOLOGY; PROCEDURES; TOXOPLASMA; TOXOPLASMOSIS, OCULAR; YOUNG ADULT;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BLOOD; DNA, PROTOZOAN; EYE; FEMALE; HUMANS; IMMUNOBLOTTING; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TOXOPLASMA; TOXOPLASMOSIS, OCULAR; YOUNG ADULT;

EID: 84908332241     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01793-14     Document Type: Article

References (23)

1. Bodaghi B, Touitou V, Fardeau C, Paris L, LeHoang P. 2012. Toxoplasmosis: new challenges for an old disease. Eye (Lond). 26:241-244. http://dx.doi.org/10.1038/eye.2011.331.
2. McCannel CA, Holland GN, Helm CJ, Cornell PJ, Winston JV, Rimmer TG. 1996. Causes of uveitis in the general practice of ophthalmology. UCLA Community-Based Uveitis Study Group. Am. J. Ophthalmol. 121:35-46.
3. Montoya JG, Remington JS. 1996. Toxoplasmic chorioretinitis in the setting of acute acquired toxoplasmosis. Clin. Infect. Dis. 23:277-282. http://dx.doi.org/10.1093/clinids/23.2.277.
4. Delair E, Monnet D, Grabar S, Dupouy-Camet J, Yera H, Brezin AP. 2008. Respective roles of acquired and congenital infections in presumed ocular toxoplasmosis. Am. J. Ophthalmol. 146:851-855. http://dx.doi.org/10.1016/j.ajo.2008.06.027.
5. Garweg JG, Garweg SD, Flueckiger F, Jacquier P, Boehnke M. 2004. Aqueous humor and serum immunoblotting for immunoglobulin types G, A, M, and E in cases of human ocular toxoplasmosis. J. Clin. Microbiol. 42:4593-4598. http://dx.doi.org/10.1128/JCM.42.10.4593-4598.2004.
6. Fekkar A, Bodaghi B, Touafek F, Le Hoang P, Mazier D, Paris L. 2008. Comparison of immunoblotting, calculation of the Goldmann-Witmer coefficient, and real-time PCR using aqueous humor samples for diagnosis of ocular toxoplasmosis. J. Clin. Microbiol. 46:1965-1967. http://dx.doi.org/10.1128/JCM.01900-07.
7. Talabani H, Asseraf M, Yera H, Delair E, Ancelle T, Thulliez P, Brezin AP, Dupouy-Camet J. 2009. Contributions of immunoblotting, real-time PCR, and the Goldmann-Witmer coefficient to diagnosis of atypical toxoplasmic retinochoroiditis. J. Clin. Microbiol. 47:2131-2135. http://dx.doi.org/10.1128/JCM.00128-09.
8. Bou G, Figueroa MS, Marti-Belda P, Navas E, Guerrero A. 1999. Value of PCR for detection of Toxoplasma gondii in aqueous humor and blood samples from immunocompetent patients with ocular toxoplasmosis. J. Clin. Microbiol. 37:3465-3468.
9. Contini C, Seraceni S, Cultrera R, Incorvaia C, Sebastiani A, Picot S. 2005. Evaluation of a real-time PCR-based assay using the LightCycler system for detection of Toxoplasma gondii bradyzoite genes in blood specimens from patients with toxoplasmic retinochoroiditis. Int. J. Parasitol. 35:275-283. http://dx.doi.org/10.1016/j.ijpara.2004.11.016.
10. Cassaing S, Bessieres MH, Berry A, Berrebi A, Fabre R, Magnaval JF. 2006. Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR. J. Clin. Microbiol. 44:720-724. http://dx.doi.org/10.1128/JCM.44.3.720-724.2006.
11. De Groot-Mijnes JD, Rothova A, Van Loon AM, Schuller M, Ten Dam-Van Loon NH, De Boer JH, Schuurman R, Weersink AJ. 2006. Polymerase chain reaction and Goldmann-Witmer coefficient analysis are complimentary for the diagnosis of infectious uveitis. Am. J. Ophthalmol. 141:313-318. http://dx.doi.org/10.1016/j.ajo.2005.09.017.
12. Westeneng AC, Rothova A, de Boer JH, de Groot-Mijnes JD. 2007. Infectious uveitis in immunocompromised patients and the diagnostic value of polymerase chain reaction and Goldmann-Witmer coefficient in aqueous analysis. Am. J. Ophthalmol. 144:781-785. http://dx.doi.org/10.1016/j.ajo.2007.06.034.
13. Mesquita RT, Vidal JE, Pereira-Chioccola VL. 2010. Molecular diagnosis of cerebral toxoplasmosis: comparing markers that determine Toxoplasma gondii by PCR in peripheral blood from HIV-infected patients. Braz. J. Infect. Dis. 14:346-350. http://dx.doi.org/10.1016/S1413-8670(10)70073-8.
14. Wallon M, Franck J, Thulliez P, Huissoud C, Peyron F, Garcia-Meric P, Kieffer F. 2010. Accuracy of real-time polymerase chain reaction for Toxoplasma gondii in amniotic fluid. Obstet. Gynecol. 115:727-733. http://dx.doi.org/10.1097/AOG.0b013e3181d57b09.
15. Lee SE, Hong SH, Lee SH, Jeong YI, Lim SJ, Kwon OW, Kim SH, You YS, Cho SH, Lee WJ. 2012. Detection of ocular Toxoplasma gondii infection in chronic irregular recurrent uveitis by PCR. Korean J. Parasitol. 50:229-231. http://dx.doi.org/10.3347/kjp.2012.50.3.229.
16. Ajzenberg D. 2010. Is PCR testing on blood samples useful or not in the diagnosis of Toxoplasma encephalitis? Trans. R. Soc. Trop. Med. Hyg. 104:569-570. http://dx.doi.org/10.1016/j.trstmh.2010.04.003.
17. Colombo FA, Vidal JE, Penalva de Oliveira AC, Hernandez AV, Bo-nasser-Filho F, Nogueira RS, Focaccia R, Pereira-Chioccola VL. 2005. Diagnosis of cerebral toxoplasmosis in AIDS patients in Brazil: importance of molecular and immunological methods using peripheral blood samples. J. Clin. Microbiol. 43:5044-5047. http://dx.doi.org/10.1128/JCM.43.10.5044-5047.2005.
18. Correia CC, Melo HR, Costa VM. 2010. Influence of neurotoxoplasmosis characteristics on real-time PCR sensitivity among AIDS patients in Brazil. Trans. R. Soc. Trop. Med. Hyg. 104:24-28. http://dx.doi.org/10.1016/j.trstmh.2009.06.012.
19. Martino R, Bretagne S, Einsele H, Maertens J, Ullmann AJ, Parody R, Schumacher U, Pautas C, Theunissen K, Schindel C, Munoz C, Margall N, Cordonnier C. 2005. Early detection of Toxoplasma infection by molecular monitoring of Toxoplasma gondii in peripheral blood samples after allogeneic stem cell transplantation. Clin. Infect. Dis. 40:67-78. http://dx.doi.org/10.1086/426447.
20. Silveira C, Vallochi AL, Rodrigues da Silva U, Muccioli C, Holland GN, Nussenblatt RB, Belfort R, Rizzo LV. 2011. Toxoplasma gondii in the peripheral blood of patients with acute and chronic toxoplasmosis. Br. J. Ophthalmol. 95:396-400. http://dx.doi.org/10.1136/bjo.2008.148205.
21. Ferreira IM, Vidal JE, de Mattos CDCB, de Mattos LC, Qu D, Su C, Pereira-Chioccola VL. 2011. Toxoplasma gondii isolates: multilocus RFLP-PCR genotyping from human patients in Sao Paulo State, Brazil identified distinct genotypes. Exp. Parasitol. 129:190-195. http://dx.doi.org/10.1016/j.exppara.2011.06.002.
22. Mattos CC, Meira CS, Ferreira AI, Frederico FB, Hiramoto RMGC, Jr, Mattos LC, Pereira-Chioccola VL. 2011. Contribution of laboratory methods in diagnosing clinically suspected ocular toxoplasmosis in Brazilian patients. Diagn. Microbiol. Infect. Dis. 70:362-366. http://dx.doi.org/10.1016/j.diagmicrobio.2011.02.002.
23. Fekkar A, Ajzenberg D, Bodaghi B, Touafek F, Le Hoang P, Delmas J, Robert PY, Darde ML, Mazier D, Paris L. 2011. Direct genotyping of Toxoplasma gondii in ocular fluid samples from 20 patients with ocular toxoplasmosis: predominance of type II in France. J. Clin. Microbiol. 49:1513-1517. http://dx.doi.org/10.1128/JCM.02196-10.