Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR

Journal of Clinical Microbiology

Volumn 44, Issue 3, 2006, Pages 720-724

  Cassaing, S ^a   Bessieres M H ^a   Berry, A ^a   Berrebi, A ^b   Fabre, R ^a   Magnaval, J F ^a  

^a CHU RANGUEIL   (France)

^b Paul Sabatier University   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMNION FLUID; AQUEOUS HUMOR; ARTICLE; BLOOD; CEREBROSPINAL FLUID; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNE DEFICIENCY; INTERMETHOD COMPARISON; LUNG LAVAGE; MAJOR CLINICAL STUDY; NUCLEOTIDE SEQUENCE; PLACENTA; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; TOXOPLASMA; TOXOPLASMOSIS;

AMNIOTIC FLUID; ANIMALS; AQUEOUS HUMOR; BASE SEQUENCE; BRONCHOALVEOLAR LAVAGE FLUID; DNA, PROTOZOAN; FEMALE; GENES, PROTOZOAN; HUMANS; IMMUNOCOMPROMISED HOST; INFANT, NEWBORN; PLACENTA; POLYMERASE CHAIN REACTION; PREGNANCY; PREGNANCY COMPLICATIONS, PARASITIC; PRENATAL DIAGNOSIS; SENSITIVITY AND SPECIFICITY; TOXOPLASMA; TOXOPLASMOSIS; TOXOPLASMOSIS, CONGENITAL; TOXOPLASMOSIS, OCULAR;

TOXOPLASMA;

EID: 33644911806     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.44.3.720-724.2006     Document Type: Article

References (23)

1. Bastien, P. 2002. Molecular diagnosis of toxoplasmosis. Trans. R. Soc. Trop. Med. Hyg. 96(Suppl. 1):S205-S215.
2. Bessières, M. H., S. Le Breton, and J. P. Séguéla. 1992. Analysis by immunoblotting of Toxoplasma gondii exo-antigens and comparison with somatic antigens. Parasitol. Res. 78:222-228.
3. Bessières, M. H., A. Berrebi, M. Rolland, M. C. Bloom, C. Roques, S. Cassaing, C. Courjault, and J. P. Séguéla. 2001. Neonatal screening for congenital toxoplasmosis in a cohort of 165 women infected during pregnancy and influence of in utero treatment on the results of neonatal tests. Eur. J. Obstet. Gynecol. Reprod. Biol. 94:37-45.
4. Botterel, F., P. Ichai, C. Feray, P. Bouree, F. Saliba, R. Tur Raspa, D. Samuel, and S. Romand. 2002. Disseminated toxoplasmosis, resulting from infection of allograft, after orthotopic liver transplantation: usefulness of quantitative PCR. J. Clin. Microbiol. 40:1648-1650.
5. Buchbinder, S., R. Blatz, and A. C. Rodloff. 2003. Comparison of real time PCR detection methods for B1 and P30 genes of Toxoplasma gondii. Diagn. Microbiol. Infect. Dis. 45:269-271.
6. Burg, J. L., C. M. Grover, P. Pouletty, and J. C. Boothrood. 1989. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J. Clin. Microbiol. 27:1787-1792.
7. Chabbert, E., L. Lachaud, L. Crobu, and P. Bastien. 2004. Comparison of two widely used PCR primer systems for detection of Toxoplasma in amniotic fluid, blood, and tissues. J. Clin. Microbiol. 42:1719-1722.
8. Costa, J. M., C. Pautas, P. Ernault, F. Foulet, C. Cordonnier, and S. Bretagne. 2000. Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes. J. Clin. Microbiol. 38:2929-2932.
9. Costa, J. M., C. Munoz, D. Krüger, R. Martino, T. K. Held, M. L. Darde, C. Cordonnier, and S. Bretagne on behalf of the Infectious Diseases Working Party of the European Group for Blood and Marrow Transplantation. 2001. Quality control for the diagnosis of Toxoplasma gondii reactivation in SCT patients using PCR assays. Bone Marrow Transpl. 28:527-528.
10. Costa, J. M., P. Ernault, E. Gautier, and S. Bretagne. 2001. Prenatal diagnosis of congenital toxoplasmosis by duplex real time PCR using fluorescence resonance energy transfer hybridization probes. Prenat. Diagn. 21:85-88.
11. Desmonts, G., and J. Couvreur. 1974. L'isolement du parasite dans la toxoplasmose congénitale: intérêt pratique et théorique. Arch. Fr. Pédiatr. 31:157-166.
12. Hierl, T., U. Reischl, P. Lang, H. Hebart, M. Stark, P. Kyme, and I. B. Autenrieth. 2004. Preliminary evaluation of one conventional nested and two real time PCR assays for the detection of Toxoplasma gondii in immunocompromised patients. J. Med. Microbiol. 53:1-4.
13. Hohlfeld, P., F. Daffos, J. M. Costa, P. Thulliez, F. Forestier, and M. Vidaud. 1994. Prenatal diagnosis of congenital toxoplasmosis with a polymerase chain reaction test on amniotic fluid. N. Engl. J. Med. 331:695-699.
14. Homan, W. L., M. Vercammen, J. De Braekeler, and H. Verschueren. 2000. Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int. J. Parasitol. 30:69-75.
15. Janitschke, K., T. Held, D. Kruiger, R. Schwerdtfeger, G. Schlier, and O. Liesenfeld. 2003. Diagnostic value of tests for Toxoplasma gondii specific antibodies in patients undergoing bone marrow transplantation. Clin. Lab. 49:239-242.
16. Jones, C. D., N. Okhravi, P. Adamson, S. Tasker, and S. Lightman. 1999. Comparison of PCR detection methods for B1, P30 and 18s rDNA genes of Toxoplasma gondii in aqueous humor. Investig. Ophthalmol. Vis. Sci. 41:634-644.
17. Lin, M. H., T. C. Chen, T. T. Kuo, C. C. Tseng, and C. P. Tseng. 2000. Real time PCR for quantitative detection of Toxoplasma gondii. J. Clin. Microbiol. 38:4121-4125.
18. Martino, R., S. Bretagne, H. Einsele, J. Maertens, A. J. Ullmann, R. Parody, U. Schumacher, C. Pautas, K. Theunissen, C. Schindel, C. Munoz, N. Margali, and C. Cordonnier. 2005. Early detection of Toxoplasma infection by molecular monitoring of Toxoplasma gondii in peripheral blood samples after allogeneic stem cell transplantation. Clin. Infect. Dis. 40:67-78.
19. Menotti, J., G. Vilela, S. Romand, Y. J. F. Garin, L. Ades, E. Gluckmann, F. Derouin, and P. Ribaud. 2003. Comparison of PCR-enzyme-linked immunosorbent assay and real-time PCR assay for diagnosis of an unusual case of cerebral toxoplasmosis in a stem cell transplant recipient. J. Clin. Microbiol. 41:5313-5316.
20. Miedouge, M., M. H. Bessières, S. Cassaing, B. Swierczynski, and J. P. Séguéla. 1997. Parasitemia and parasitic loads in acute infection and after anti-gamma-interferon treatment in a toxoplasmic mouse model. Parasitol. Res. 83:339-344.
21. Reischl, U., S. Bretagne, D. Krüger, P. Ernault, and J. M. Costa. 2003. Comparison of two DNA targets for the diagnosis of toxoplasmosis by real time PCR using fluorescence resonance energy transfer hybridization probes. BMC Infect. Dis. 3:7.
22. Sambrook, A., B. F. Fritsch, and T. Maniatis. 1989. Purification of nucleic acids, p. E3-E10. In N. Ford, C. Nolan, and M. Ferguson (ed.), Molecular cloning: a laboratory manual, vol. 3. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
23. Simon, A., P. Labalette, I. Ordinaire, E. Fréalle, E. Dei-Cas, D. Camus, and L. Delhaes. 2004. Use of fluorescence resonance energy transfer hybridization probes to evaluate quantitative real-time PCR for diagnosis of ocular toxoplasmosis. J. Clin. Microbiol. 42:3681-3685.