Contributions of immunoblotting, real-time PCR, and the Goldmann-Witmer coefficient to diagnosis of atypical toxoplasmic retinochoroiditis

Journal of Clinical Microbiology

Volumn 47, Issue 7, 2009, Pages 2131-2135

  Talabani, H ^a   Asseraf, M ^a   Yera, H ^a   Delair, E ^a   Ancelle, T ^a   Thulliez, P ^b   Brezin A P ^a   Dupouy Camet, J ^a  

^a université Paris Cité   (France)

^b Institut de Puericulture   (France)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; PROTOZOAL DNA;

ADOLESCENT; ADULT; AGED; ANALYTICAL PARAMETERS; ANTERIOR EYE CHAMBER; ANTIBODY PRODUCTION; AQUEOUS HUMOR; ARTICLE; BLOOD SAMPLING; CHORIORETINITIS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; FEMALE; GOLDMANN WITMER COEFFICIENT; HUMAN; IMMUNE DEFICIENCY; IMMUNOBLOTTING; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; NUCLEOTIDE SEQUENCE; OPTIC DISK; PARACENTESIS; PARASITE IDENTIFICATION; PRIORITY JOURNAL; PUNCTURE; REAL TIME POLYMERASE CHAIN REACTION; RETINA NECROSIS; TOXOPLASMIC RETINOCHOROIDITIS; TOXOPLASMOSIS; UVEITIS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANIMALS; CHOROIDITIS; FEMALE; HUMANS; IMMUNOBLOTTING; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; RETINITIS; SENSITIVITY AND SPECIFICITY; TOXOPLASMA; TOXOPLASMOSIS; TOXOPLASMOSIS, OCULAR; YOUNG ADULT;

TOXOPLASMA GONDII;

EID: 67650076202     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00128-09     Document Type: Article

References (21)

1. Brezin, A. P., C. E. Eqwuagu, C. Silveira, P. Thulliez, M. C. Martins, R. M. Mahdi, R. Belfort, Jr., and R. B. Nussenblatt. 1991. Analysis of aqueous humor in ocular toxoplasmosis. N. Engl. J. Med. 324:699.
2. Cassaing, S., M. H. Bessieres, A. Berry, A. Berrebi, R. Fabre, and J. F. Magnaval. 2006. Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR. J. Clin. Microbiol. 44:720-724.
3. de Boer, J. H., C. Verhagen, M. Bruinenberg, A. Rothova, P. T. de Jong, G. S. Baarsma, A. Van der Lelij, F. M. Ooyman, J. G. Bollemeijer, P. J. Derhaag, and A. Kijlstra. 1996. Serologic and polymerase chain reaction analysis of intraocular fluids in the diagnosis of infectious uveitis. Am. J. Ophthalmol. 121:650-658.
4. De Groot-Mijnes, J. D., A. Rothova, A. M. Van Loon, M. Schuller, N. H. Ten Dam-Van Loon, J. H. De Boer, R. Schuurman, and A. J. Weersink. 2006. Polymerase chain reaction and Goldmann-Witmer coefficient analysis are complimentary for the diagnosis of infectious uveitis. Am. J. Ophthalmol. 141:313-318.
5. Delair, E., D. Monnet, S. Grabar, J. Dupouy-Camet, H. Yera, and A. P. Brezin. 2008. Respective roles of acquired and congenital infections in presumed ocular toxoplasmosis. Am. J. Ophthalmol. 146:851-855.
6. Desmonts, G., A. Baron, G. Offret, J. Couvreur, and M. Lelong. 1960. The local production of antibodies in the course of ocular toxoplasmosis. Arch. Ophthalmol Rev. Gen. Ophthalmol. 20:137-145. (In French.)
7. Edvinsson, B., S. Jalal, C. E. Nord, B. S. Pedersen, and B. Evengard. 2004. DNA extraction and PCR assays for detection of Toxoplasma gondii. APMIS 112:342-348.
8. Fardeau, C., S. Romand, N. A. Rao, N. Cassoux, O. Bettembourg, P. Thulliez, and P. Lehoang. 2002. Diagnosis of toxoplasmic retinochoroiditis with atypical clinical features. Am. J. Ophthalmol. 134:196-203.
9. Fekkar, A., B. Bodaghi, F. Touafek, P. Le Hoang, D. Mazier, and L. Paris. 2008. Comparison of immunoblotting, calculation of the Goldmann-Witmer coefficient, and real-time PCR using aqueous humor samples for diagnosis of ocular toxoplasmosis. J. Clin. Microbiol. 46:1965-1967.
10. Garweg, J. G., S. D. Garweg, F. Flueckiger, P. Jacquier, and M. Boehnke. 2004. Aqueous humor and serum immunoblotting for immunoglobulin types G, A, M, and E in cases of human ocular toxoplasmosis. J. Clin. Microbiol. 42:4593-4598.
11. Garweg, J. G., P. Jacquier, and M. Boehnke. 2000. Early aqueous humor analysis in patients with human ocular toxoplasmosis. J. Clin. Microbiol. 38:996-1001.
12. Holland, G. N. 1999. Reconsidering the pathogenesis of ocular toxoplasmosis. Am. J. Ophthalmol. 128:502-505.
13. Holland, G. N., R. E. Engstrom, Jr., B. J. Glasgow, B. B. Berger, S. A. Daniels, Y. Sidikaro, J. A. Harmon, D. H. Fischer, D. S. Boyer, N. A. Rao, et al. 1988. Ocular toxoplasmosis in patients with the acquired immunodeficiency syndrome. Am. J. Ophthalmol. 106:653-667.
14. Labalette, P., L. Delhaes, F. Margaron, B. Fortier, and J. F. Rouland. 2002. Ocular toxoplasmosis after the fifth decade. Am. J. Ophthalmol. 133:506-515.
15. McCannel, C. A., G. N. Holland, C. J. Helm, P. J. Cornell, J. V. Winston, T. G. Rimmer, et al. 1996. Causes of uveitis in the general practice of ophthalmology. Am. J. Ophthalmol. 121:35-46.
16. Riss, J. M., M. E. Carboni, J. Y. Franck, C. J. Mary, H. Dumon, and B. Ridings. 1995. Ocular toxoplasmosis: value of immunoblotting for the determination of an intra-ocular synthesis of antibodies. Pathol. Biol. (Paris) 43:772-778. (In French.)
17. Robert-Gangneux, F., P. Binisti, D. Antonetti, A. Brezin, H. Yera, and J. Dupouy-Camet. 2004. Usefulness of immunoblotting and Goldmann-Witmer coefficient for biological diagnosis of toxoplasmic retinochoroiditis. Eur. J. Clin. Microbiol. Infect. Dis. 23:34-38.
18. Rothova, A., J. H. de Boer, N. H. Ten Dam-van Loon, G. Postma, L. de Visser, S. J. Zuurveen, M. Schuller, A. J. Weersink, A. M. van Loon, and J. D. de Groot-Mijnes. 2008. Usefulness of aqueous humor analysis for the diagnosis of posterior uveitis. Ophthalmology 115:306-311.
19. Stanford, M. R., H. K. Tan, and R. E. Gilbert. 2006. Toxoplasmic retinochoroiditis presenting in childhood: clinical findings in a UK survey. Br. J. Ophthalmol. 90:1464-1467.
20. Villard, O., D. Filisetti, F. Roch-Deries, J. Garweg, J. Flament, and E. Candolfi. 2003. Comparison of enzyme-linked immunosorbent assay, immunoblotting, and PCR for diagnosis of toxoplasmic chorioretinitis. J. Clin. Microbiol. 41:3537-3541.
21. Westeneng, A. C., A. Rothova, J. H. de Boer, and J. D. de Groot-Mijnes. 2007. Infectious uveitis in immunocompromised patients and the diagnostic value of polymerase chain reaction and Goldmann-Witmer coefficient in aqueous analysis. Am. J. Ophthalmol. 144:781-785.