Using a new Lrig1 reporter mouse to assess differences between two Lrig1 antibodies in the intestine

Stem Cell Research

Volumn , Issue Part A, 2014, Pages 422-430

  Poulin, Emily J ^a   Powell, Anne E ^b   Wang, Yang ^b   Li, Yina ^b   Franklin, Jeffrey L ^a   Coffey, Robert J ^a,b,c  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VANDERBILT UNIVERSITY MEDICAL CENTER   (United States)

^c VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; LEUCINE RICH AND IMMUNOGLOBULIN LIKE DOMAINS PROTEIN 1 ANTIBODY; RED FLUORESCENT PROTEIN; UNCLASSIFIED DRUG; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; LRIG1 PROTEIN, MOUSE; MEMBRANE PROTEIN; NERVE PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOCHEMISTRY; CELL POPULATION; CONTROLLED STUDY; EPITHELIUM CELL; EXPERIMENTAL MOUSE; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; HUMAN CELL; INTESTINE; LRIG1 REPORTER MOUSE; MOUSE; NONHUMAN; PROTEIN GLYCOSYLATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ANIMAL; CYTOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; GLYCOSYLATION; HEK293 CELL LINE; IMMUNOLOGY; METABOLISM; STEM CELL;

ANIMALS; ANTIBODIES; EPITHELIAL CELLS; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; GLYCOSYLATION; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; INTESTINES; MEMBRANE GLYCOPROTEINS; MICE; NERVE TISSUE PROTEINS; STEM CELLS;

EID: 84908266005     PISSN: 18735061     EISSN: 18767753     Source Type: Journal    
DOI: 10.1016/j.scr.2014.09.002     Document Type: Article

References (18)

1. Barker N., van Es J.H., Kuipers J., Kujala P., van den Born M., Cozijnsen M., Haegebarth A., Korving J., Begthel H., Peters P.J., et al. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 2007, 449:1003-1007.
2. Breault D.T., Min I.M., Carlone D.L., Farilla L.G., Ambruzs D.M., Henderson D.E., Algra S., Montgomery R.K., Wagers A.J., Hole N. Generation of mTert-GFP mice as a model to identify and study tissue progenitor cells. Proc. Natl. Acad. Sci. U. S. A. 2008, 105:10420-10425.
3. Daniels M.A., Hogquist K.A., Jameson S.C. Sweet 'n' sour: the impact of differential glycosylation on T cell responses. Nat. Immunol. 2002, 3:903-910.
4. Davies P.S., Powell A.E., Swain J.R., Wong M.H. Inflammation and proliferation act together to mediate intestinal cell fusion. PLoS ONE 2009, 4:e6530.
5. Gur G., Rubin C., Katz M., Amit I., Citri A., Nilsson J., Amariglio N., Henriksson R., Rechavi G., Hedman H., et al. LRIG1 restricts growth factor signaling by enhancing receptor ubiquitylation and degradation. EMBO J. 2004, 23:3270-3281.
6. Hakomori S. Glycosylation defining cancer malignancy: new wine in an old bottle. Proc. Natl. Acad. Sci. U. S. A. 2002, 99:10231-10233.
7. Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T. Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins. Nat. Biotechnol. 2003, 21:667-672.
8. Kaji H., Yamauchi Y., Takahashi N., Isobe T. Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site-specific stable isotope tagging. Nat. Protoc. 2006, 1:3019-3027.
9. Kaji H., Shikanai T., Sasaki-Sawa A., Wen H., Fujita M., Suzuki Y., Sugahara D., Sawaki H., Yamauchi Y., Shinkawa T., et al. Large-scale identification of N-glycosylated proteins of mouse tissues and construction of a glycoprotein database, GlycoProtDB. J. Proteome Res. 2012, 11:4553-4566.
10. Li Y., Zhang H., Choi S.C., Litingtung Y., Chiang C. Sonic hedgehog signaling regulates Gli3 processing, mesenchymal proliferation, and differentiation during mouse lung organogenesis. Dev. Biol. 2004, 270:214-231.
11. Nilsson J., Vallbo C., Guo D., Golovleva I., Hallberg B., Henriksson R., Hedman H. Cloning, characterization, and expression of human LIG1. Biochem. Biophys. Res. Commun. 2001, 284:1155-1161.
12. Nilsson J., Starefeldt A., Henriksson R., Hedman H. LRIG1 protein in human cells and tissues. Cell Tissue Res. 2003, 312:65-71.
13. Ohtsubo K., Marth J.D. Glycosylation in cellular mechanisms of health and disease. Cell 2006, 126:855-867.
14. Powell A.E., Anderson E.C., Davies P.S., Silk A.D., Pelz C., Impey S., Wong M.H. Fusion between Intestinal epithelial cells and macrophages in a cancer context results in nuclear reprogramming. Cancer Res. 2011, 71:1497-1505.
15. Powell A.E., Wang Y., Li Y., Poulin E.J., Means A.L., Washington M.K., Higginbotham J.N., Juchheim A., Prasad N., Levy S.E., et al. The pan-ErbB negative regulator Lrig1 is an intestinal stem cell marker that functions as a tumor suppressor. Cell 2012, 149:146-158.
16. Tan C.W., Hirokawa Y., Gardiner B.S., Smith D.W., Burgess A.W. Colon cryptogenesis: asymmetric budding. PLoS ONE 2013, 8:e78519.
17. Whitehead R.H., Brown A., Bhathal P.S. A method for the isolation and culture of human colonic crypts in collagen gels. In Vitro Cell. Dev. Biol. 1987, 23:436-442.
18. Wong V.W., Stange D.E., Page M.E., Buczacki S., Wabik A., Itami S., van de Wetering M., Poulsom R., Wright N.A., Trotter M.W., et al. Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling. Nat. Cell Biol. 2012, 14:401-408.