A high performance, cost-effective, open-source microscope for scanning two-photon microscopy that is modular and readily adaptable

PLoS ONE

Volumn 9, Issue 10, 2014, Pages

  Rosenegger, David G ^a   Tran, Cam Ha T ^a   LeDue, Jeffery ^b   Zhou, Ning ^c   Gordon, Grant R ^a  

^a UNIVERSITY OF CALGARY   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c CHINA MEDICAL UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTICAL EQUIPMENT; ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; CLINICAL PROTOCOL; COMPUTER MODEL; COMPUTER PROGRAM; COST EFFECTIVENESS ANALYSIS; EQUIPMENT DESIGN; IMAGE DISPLAY; IN VITRO STUDY; IN VIVO STUDY; KNOWLEDGE BASE; LABORATORY DEVICE; LASER MICROSCOPE; LASER MICROSCOPY; LENS; MOUSE; NONHUMAN; OPTICAL INSTRUMENTATION; SIGNAL NOISE RATIO; TWO PHOTON LASER SCANNING FLUORESCENCE MICROSCOPE; TWO PHOTON LASER SCANNING MICROSCOPY; ANIMAL; BRAIN; DEVICES; ECONOMICS; IMAGE PROCESSING; PATHOLOGY; PHOTON; PROCEDURES; SCANNING ELECTRON MICROSCOPY;

ANIMALS; BRAIN; IMAGE PROCESSING, COMPUTER-ASSISTED; MICE; MICROSCOPY, ELECTRON, SCANNING; PHOTONS; SIGNAL-TO-NOISE RATIO; SOFTWARE;

EID: 84908190358     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0110475     Document Type: Article

References (36)

1. Helmchen F, Denk W (2005) Deep tissue two-photon microscopy. Nat Methods 2: 932-940. doi:10.1038/nmeth818.
2. So PT, Dong CY, Masters B.R., Berland KM (2000) Two-photon excitation fluorescence microscopy. Annu Rev Biomed Eng 2: 399-429. doi:10.1146/ annurev.bioeng.2.1.399.
3. Denk W, Strickler JH, Webb WW (1990) Two-photon laser scanning fluorescence microscopy. Science 248: 73-76.
4. Soeller C, Cannell MB (1996) Construction of a two-photon microscope and optimisation of illumination pulse duration. Pflugers Arch 432: 555-561.
5. Konig K, Simon U, Halbhuber KJ (1996) 3D resolved two-photon fluorescence microscopy of living cells using a modified confocal laser scanning microscope. Cell Mol Biol (Noisy-le-grand) 42: 1181-1194.
6. Mainen ZF, Maletic-Savatic M, Shi S.H., Hayashi Y., Malinow R, et al. (1999) Two-photon imaging in living brain slices. Methods 18: 231-9-181.
7. Tsai PS, Nishimura N, Yoder E.J., White A., Dolnick E, et al. (2002) Principles, design and construction of a two photon scanning microscope for in vitro and in vivo studies. In Methods for In Vivo Optical Imaging of Brain Function, Frostig R (2002). CRC Press. 113-171.
8. Zipfel WR, Williams RM, Webb WW (2003) Nonlinear magic: multiphoton microscopy in the biosciences. Nat Biotechnol 21: 1369-1377. doi:10.1038/ nbt899.
9. Kim KH, Buehler C, So PT (1999) High-speed, two-photon scanning microscope. Appl Opt 38: 6004-6009.
10. Nguyen QT, Callamaras N, Hsieh C., Parker I (2001) Construction of a two-photon microscope for video-rate Ca(2+) imaging. Cell Calcium 30: 383-393.
11. Jiang R, Zhou Z, Lv X., Zeng S (2012) Wide-band acousto-optic deflectors for large field of view two-photon microscope. Rev Sci Instrum 83: 043709. doi:10.1063/1.4705972.
12. Tan YP, Llano I, Hopt A., Wurriehausen F, Neher E (1999) Fast scanning and efficient photodetection in a simple two-photon microscope. J Neurosci Methods 92: 123-135.
13. Driscoll JD, Shih AY, Iyengar S, Field J.J., White GA, et al. (2011) Photon counting, censor corrections, and lifetime imaging for improved detection in two-photon microscopy. J Neurophysiol 105: 3106-3113. doi:10.1152/ jn.00649.2010.
14. Kobat D, Horton NG, Xu C (2011) In vivo two-photon microscopy to 1.6-mm depth in mouse cortex. J Biomed Opt 16: 106014. doi:10.1117/1.3646209.
15. Theer P, Hasan MT, Denk W (2003) Two-photon imaging to a depth of 1000 microm in living brains by use of a Ti: Al2O3 regenerative amplifier. Opt Lett 28: 1022-1024.
16. Oheim M, Beaurepaire E, Chaigneau E., Mertz J, Charpak S (2001) Two-photon microscopy in brain tissue: parameters influencing the imaging depth. J Neurosci Methods 111: 29-37.
17. Nikolenko V, Yuste R (2013) How to build a two-photon microscope with a confocal scan head. Cold Spring Harb Protoc 2013. doi:10.1101/pdb.ip075135.
18. Diaspro A, Corosu M, Ramoino P., Robello M (1999) Adapting a compact confocal microscope system to a two-photon excitation fluorescence imaging architecture. Microsc Res Tech 47: 196-205. doi:10.1002/(SICI)1097-0029(19991101)47:3, 196::AID-JEMT5.3.0.CO;2-V.
19. Wier WG, Balke CW, Michael J.A., Mauban JR (2000) A custom confocal and two-photon digital laser scanning microscope. Am J Physiol Heart Circ Physiol 278: H2150-H2156.
20. Majewska A, Yiu G, Yuste R (2000) A custom-made two-photon microscope and deconvolution system. Pflugers Arch 441: 398-408.
21. Pologruto TA, Sabatini BL, Svoboda K (2003) ScanImage: flexible software for operating laser scanning microscopes. Biomed Eng Online 2: 13. doi:10.1186/ 1475-925X-2-13.
22. Langer D, van't Hoff M, Keller AJ, Nagaraja C, Pfäffli OA, et al. (2013) HelioScan: a software framework for controlling in vivo microscopy setups with high hardware flexibility, functional diversity and extendibility. J Neurosci Methods 215: 38-52. doi:10.1016/j.jneumeth.2013.02.006.
23. Nguyen QT, Tsai PS, Kleinfeld D (2006) MPScope: a versatile software suite for multiphoton microscopy. J Neurosci Methods.
24. Beaurepaire E, Mertz J (2002) Epifluorescence collection in two-photon microscopy. Appl Opt 41: 5376-5382.
25. Pettit DL, Wang SS, Gee K.R., Augustine GJ (1997) Chemical two-photon uncaging: a novel approach to mapping glutamate receptors. Neuron 19: 465-471.
26. Coscoy S, Waharte F, Gautreau A., Martin M, Louvard D, et al. (2002) Molecular analysis of microscopic ezrin dynamics by two-photon FRAP. Proc Natl Acad Sci USA 99: 12813-12818. doi:10.1073/pnas.192084599.
27. Cole RW, Jinadasa T, Brown CM (2011) Measuring and interpreting point spread functions to determine confocal microscope resolution and ensure quality control. Nat Protoc 6: 1929-1941. doi:10.1038/nprot.2011.407.
28. Gordon GRJ, Iremonger KJ, Kantevari S, Ellis-Davies GCR, MacVicar BA, et al. (2009) Astrocyte-mediated distributed plasticity at hypothalamic glutamate synapses. Neuron 64: 391-403. doi:10.1016/j.neuron.2009.10.021.
29. Tian L, Hires SA, Mao T, Huber D., Chiappe ME, et al. (2009) Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Nat Methods 6: 875-881. doi:10.1038/nmeth.1398.
30. Holtmaat A, Bonhoeffer T, Chow D.K., Chuckowree J., De Paola V, et al. (2009) Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Nat Protoc 4: 1128-1144. doi:10.1038/nprot.2009.89.
31. Kitamura K, Judkewitz B, Kano M., Denk W, Häusser M (2008) Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo. Nat Methods 5: 61-67. doi:10.1038/nmeth1150.
32. MacVicar BA, Hochman D (1991) Imaging of synaptically evoked intrinsic optical signals in hippocampal slices. J Neurosci 11: 1458-1469.
33. Zhou N, Gordon GRJ, Feighan D, MacVicar BA (2010) Transient swelling, acidification, and mitochondrial depolarization occurs in neurons but not astrocytes during spreading depression. Cereb Cortex 20: 2614-2624. doi:10.1093/cercor/bhq018.
34. Heider B, Nathanson JL, Isacoff E.Y., Callaway EM, Siegel RM (2010) Two-photon imaging of calcium in virally transfected striate cortical neurons of behaving monkey. PLoS ONE 5: e13829. doi:10.1371/journal.pone.0013829.
35. Dombeck DA, Khabbaz AN, Collman F, Adelman T.L., Tank DW (2007) Imaging large-scale neural activity with cellular resolution in awake, mobile mice. Neuron 56: 43-57. doi:10.1016/j.neuron.2007.08.003.
36. Chen X, Nadiarynkh O, Plotnikov S., Campagnola PJ (2012) Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure. Nat Protoc 7: 654-669. doi:10.1038/nprot.2012.009.