A cutinase from Trichoderma reesei with a lid-covered active site and kinetic properties of true lipases

Journal of Molecular Biology

Volumn 426, Issue 22, 2014, Pages 3757-3772

  Roussel, Alain ^a,b   Amara, Sawsan ^a   Nyyssölä, Antti ^c   Mateos Diaz, Eduardo ^a   Blangy, Stéphanie ^a,b   Kontkanen, Hanna ^c   Westerholm Parvinen, Ann ^c   Carrière, Frédéric ^a   Cambillau, Christian ^a,b  

^a AIX MARSEILLE UNIVERSITY   (France)

^b CNRS   (France)

^c VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

Author keywords

cutinase; interfacial activation; lipase; Trichoderma reesei; X ray structure

Indexed keywords

CUTINASE; DISULFIDE; GALACTOLIPID; LONG CHAIN TRIACYLGLYCEROL; OCTYL GLUCOSIDE; PHOSPHOLIPID; PHOSPHONIC ACID DERIVATIVE; SURFACTANT; TRIACYLGLYCEROL; TRIACYLGLYCEROL LIPASE; CARBOXYLESTERASE; ENZYME INHIBITOR;

ARTICLE; CANDIDA RUGOSA; CONFORMATIONAL TRANSITION; FILAMENTOUS FUNGUS; FUSARIUM SOLANI; GALACTOMYCES GEOTRICHUM; HYPOCREA JECORINA; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SECONDARY STRUCTURE; AMINO ACID SEQUENCE; BINDING SITE; CATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYMOLOGY; KINETICS; LIPOLYSIS; METABOLISM; MOLECULAR GENETICS; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY; TRICHODERMA; X RAY CRYSTALLOGRAPHY;

HYPOCREA JECORINA;

AMINO ACID SEQUENCE; BINDING SITES; CARBOXYLIC ESTER HYDROLASES; CATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; ENZYME INHIBITORS; KINETICS; LIPASE; LIPOLYSIS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; ORGANOPHOSPHONATES; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID; TRICHODERMA;

EID: 84908078165     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2014.09.003     Document Type: Article

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