Lid opening and unfolding in human pancreatic lipase at low pH revealed by site-directed spin labeling EPR and FTIR spectroscopy

Biochemistry

Volumn 48, Issue 3, 2009, Pages 630-638

  Ranaldi, Sebastien ^a   Belle, Valérie ^a   Woudstra, Mireille ^a   Rodriguez, Jorge ^b   Guigliarelli, Bruno ^a   Sturgis, James ^c   Carriere, Frederic ^b   Fournel, Andre ^a,d  

^a AIX MARSEILLE UNIVERSITÉ   (France)

^b Centre National de la Recherche Scientifique   (France)

^c AIX MARSEILLE UNIVERSITY   (France)

^d CNRS and Aix Marseille University   (France)

Author keywords

[No Author keywords available]

Indexed keywords

COVALENT BONDS; ENZYME ACTIVITY; FOURIER TRANSFORM INFRARED SPECTROSCOPY; PARAMAGNETIC RESONANCE; PH; PLANTS (BOTANY); SULFUR COMPOUNDS; SUPERSATURATION;

ATR FT-IR SPECTROSCOPIES; DISULFIDE BRIDGE; EPR SPECTROSCOPY; FOURIER TRANSFORM INFRA REDS; FTIR SPECTROSCOPY; PANCREATIC LIPASE; SECONDARY STRUCTURES; SITE-DIRECTED SPIN LABELING;

ELECTRON SPIN RESONANCE SPECTROSCOPY;

ASPARTIC ACID; DISULFIDE; LIPID EMULSION; TRIACYLGLYCEROL LIPASE; PNLIP PROTEIN, HUMAN; WATER;

ARTICLE; CATALYSIS; ELECTRON SPIN RESONANCE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; HUMAN; INFRARED SPECTROSCOPY; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; SPIN LABELING; ADSORPTION; BIOLOGICAL MODEL; CHEMISTRY; ENZYME STABILITY; KINETICS; METABOLISM; PROTEIN CONFORMATION;

ADSORPTION; ELECTRON SPIN RESONANCE SPECTROSCOPY; ENZYME STABILITY; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; LIPASE; MODELS, BIOLOGICAL; PROTEIN CONFORMATION; PROTEIN FOLDING; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; SPIN LABELS; WATER;

EID: 59249084729     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi801250s     Document Type: Article

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