CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis

Development Growth and Differentiation

Volumn 56, Issue 7, 2014, Pages 499-510

  Sasaki, Haruka ^a   Yoshida, Keita ^a   Hozumi, Akiko ^a   Sasakura, Yasunori ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Ciona intestinalis; Ascidian; CRISPR Cas9; Knockout; Mutagenesis

Indexed keywords

MESSENGER RNA; RNA; SHORT GUIDE RNA; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; UNCLASSIFIED DRUG; PRIMER DNA;

ARTICLE; CHORDATA; CIONA INTESTINALIS; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRISPR CAS9 GENE; ELECTROPORATION; EMBRYO; EMBRYO DEVELOPMENT; EXPRESSION VECTOR; GENE EXPRESSION; GENE INACTIVATION; GENE MUTATION; GENE TARGETING; GENOME ANALYSIS; MICROINJECTION; MUTAGENESIS; NONHUMAN; ANIMAL; GENE VECTOR; GENETICS; GENOMICS; HOMEOBOX; MUTATION RATE; PHYSIOLOGY; POLYMERASE CHAIN REACTION; PROCEDURES;

ASCIDIA; CIONA; CIONA INTESTINALIS;

ANIMALS; CIONA INTESTINALIS; CRISPR-CAS SYSTEMS; DNA PRIMERS; ELECTROPORATION; GENE KNOCKOUT TECHNIQUES; GENES, HOMEOBOX; GENETIC VECTORS; GENOMICS; MUTAGENESIS; MUTATION RATE; POLYMERASE CHAIN REACTION;

EID: 84908047255     PISSN: 00121592     EISSN: 1440169X     Source Type: Journal    
DOI: 10.1111/dgd.12149     Document Type: Article

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