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Volumn 9, Issue 10, 2014, Pages

A comparison between droplet digital and quantitative PCR in the analysis of bacterial 16S load in lung tissue samples from control and COPD GOLD 2

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S; BACTERIAL DNA;

EID: 84908018392     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0110351     Document Type: Article
Times cited : (57)

References (28)
  • 2
    • 64149097786 scopus 로고    scopus 로고
    • The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments
    • Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, et al. (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55: 611-622.
    • (2009) Clin Chem , vol.55 , pp. 611-622
    • Bustin, S.A.1    Benes, V.2    Garson, J.A.3    Hellemans, J.4    Huggett, J.5
  • 3
    • 84864100140 scopus 로고    scopus 로고
    • Simple absolute quantification method correcting for quantitative PCR efficiency variations for microbial community samples
    • Brankatschk R, Bodenhausen N, Zeyer J, Burgmann H (2012) Simple absolute quantification method correcting for quantitative PCR efficiency variations for microbial community samples. Appl Environ Microbiol 78: 4481-4489.
    • (2012) Appl Environ Microbiol , vol.78 , pp. 4481-4489
    • Brankatschk, R.1    Bodenhausen, N.2    Zeyer, J.3    Burgmann, H.4
  • 4
    • 14044265394 scopus 로고    scopus 로고
    • Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
    • Bustin SA, Nolan T (2004) Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J Biomol Tech 15: 155-166.
    • (2004) J Biomol Tech , vol.15 , pp. 155-166
    • Bustin, S.A.1    Nolan, T.2
  • 5
    • 0030067991 scopus 로고    scopus 로고
    • Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR
    • Suzuki MT, Giovannoni SJ (1996) Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR. Appl Environ Microbiol 62: 625-630.
    • (1996) Appl Environ Microbiol , vol.62 , pp. 625-630
    • Suzuki, M.T.1    Giovannoni, S.J.2
  • 6
    • 81255175501 scopus 로고    scopus 로고
    • High-throughput droplet digital PCR system for absolute quantitation of DNA copy number
    • Hindson BJ, Ness KD, Masquelier DA, Belgrader P, Heredia NJ, et al. (2011) High-throughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal Chem 83: 8604-8610.
    • (2011) Anal Chem , vol.83 , pp. 8604-8610
    • Hindson, B.J.1    Ness, K.D.2    Masquelier, D.A.3    Belgrader, P.4    Heredia, N.J.5
  • 7
    • 84855982119 scopus 로고    scopus 로고
    • Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification
    • Pinheiro LB, Coleman VA, Hindson CM, Herrmann J, Hindson BJ, et al. (2012) Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification. Anal Chem 84: 1003-1011.
    • (2012) Anal Chem , vol.84 , pp. 1003-1011
    • Pinheiro, L.B.1    Coleman, V.A.2    Hindson, C.M.3    Herrmann, J.4    Hindson, B.J.5
  • 8
    • 84873456747 scopus 로고    scopus 로고
    • New assays for monitoring residual HIV burden in effectively treated individuals
    • Strain MC, Richman DD (2013) New assays for monitoring residual HIV burden in effectively treated individuals. Curr Opin HIV AIDS 8: 106-110.
    • (2013) Curr Opin HIV AIDS , vol.8 , pp. 106-110
    • Strain, M.C.1    Richman, D.D.2
  • 10
    • 84887629698 scopus 로고    scopus 로고
    • Quantification of Plasma miRNAs by Digital PCR for Cancer Diagnosis
    • Ma J, Li N, Guarnera M, Jiang F (2013) Quantification of Plasma miRNAs by Digital PCR for Cancer Diagnosis. Biomark Insights 8: 127-136.
    • (2013) Biomark Insights , vol.8 , pp. 127-136
    • Ma, J.1    Li, N.2    Guarnera, M.3    Jiang, F.4
  • 11
    • 84879450139 scopus 로고    scopus 로고
    • Development and evaluation of a next-generation digital PCR diagnostic assay for ocular Chlamydia trachomatis infections
    • Roberts CH, Last A, Molina-Gonzalez S, Cassama E, Butcher R, et al. (2013) Development and evaluation of a next-generation digital PCR diagnostic assay for ocular Chlamydia trachomatis infections. J Clin Microbiol 51: 2195-2203.
    • (2013) J Clin Microbiol , vol.51 , pp. 2195-2203
    • Roberts, C.H.1    Last, A.2    Molina-Gonzalez, S.3    Cassama, E.4    Butcher, R.5
  • 12
    • 84897100989 scopus 로고    scopus 로고
    • Respiratory viral detection and small airway inflammation in lung tissue of patients with stable, mild COPD
    • Utokaparch S, Sze MA, Gosselink JV, McDonough JE, Elliott WM, et al. (2014) Respiratory viral detection and small airway inflammation in lung tissue of patients with stable, mild COPD. COPD 11: 197-203.
    • (2014) COPD , vol.11 , pp. 197-203
    • Utokaparch, S.1    Sze, M.A.2    Gosselink, J.V.3    McDonough, J.E.4    Elliott, W.M.5
  • 13
    • 70349133092 scopus 로고    scopus 로고
    • Micro-computed tomography measurements of peripheral lung pathology in chronic obstructive pulmonary disease
    • Hogg JC, McDonough JE, Sanchez PG, Cooper JD, Coxson HO, et al. (2009) Micro-computed tomography measurements of peripheral lung pathology in chronic obstructive pulmonary disease. Proc Am Thorac Soc 6: 546-549.
    • (2009) Proc Am Thorac Soc , vol.6 , pp. 546-549
    • Hogg, J.C.1    McDonough, J.E.2    Sanchez, P.G.3    Cooper, J.D.4    Coxson, H.O.5
  • 14
    • 84862833566 scopus 로고    scopus 로고
    • Small-airway obstruction and emphysema in chronic obstructive pulmonary disease
    • McDonough JE, Yuan R, Suzuki M, Seyednejad N, Elliott WM, et al. (2011) Small-airway obstruction and emphysema in chronic obstructive pulmonary disease. N Engl J Med 365: 1567-1575.
    • (2011) N Engl J Med , vol.365 , pp. 1567-1575
    • McDonough, J.E.1    Yuan, R.2    Suzuki, M.3    Seyednejad, N.4    Elliott, W.M.5
  • 15
    • 2942720573 scopus 로고    scopus 로고
    • The nature of small-airway obstruction in chronic obstructive pulmonary disease
    • Hogg JC, Chu F, Utokaparch S, Woods R, Elliott WM, et al. (2004) The nature of small-airway obstruction in chronic obstructive pulmonary disease. N Engl J Med 350: 2645-2653.
    • (2004) N Engl J Med , vol.350 , pp. 2645-2653
    • Hogg, J.C.1    Chu, F.2    Utokaparch, S.3    Woods, R.4    Elliott, W.M.5
  • 17
    • 77649119443 scopus 로고    scopus 로고
    • Disordered microbial communities in asthmatic airways
    • Hilty M, Burke C, Pedro H, Cardenas P, Bush A, et al. (2010) Disordered microbial communities in asthmatic airways. PLoS One 5: e8578.
    • (2010) PLoS One , vol.5 , pp. e8578
    • Hilty, M.1    Burke, C.2    Pedro, H.3    Cardenas, P.4    Bush, A.5
  • 19
    • 84924172977 scopus 로고    scopus 로고
    • Enrichment of lung microbiome with supraglottic taxa is associated with increased pulmonary inflammation
    • Segal LN, Alekseyenko AV, Clemente JC, Kulkarni R, Wu B, et al. (2013) Enrichment of lung microbiome with supraglottic taxa is associated with increased pulmonary inflammation. Microbiome 1: 1-12.
    • (2013) Microbiome , vol.1 , pp. 1-12
    • Segal, L.N.1    Alekseyenko, A.V.2    Clemente, J.C.3    Kulkarni, R.4    Wu, B.5
  • 20
    • 77049098120 scopus 로고    scopus 로고
    • A persistent and diverse airway microbiota present during chronic obstructive pulmonary disease exacerbations
    • Huang YJ, Kim E, Cox MJ, Brodie EL, Brown R, et al. (2010) A persistent and diverse airway microbiota present during chronic obstructive pulmonary disease exacerbations. OMICS 14: 9-59.
    • (2010) OMICS , vol.14 , pp. 9-59
    • Huang, Y.J.1    Kim, E.2    Cox, M.J.3    Brodie, E.L.4    Brown, R.5
  • 21
    • 84867427320 scopus 로고    scopus 로고
    • The lung microbiome in moderate and severe chronic obstructive pulmonary disease
    • Pragman AA, Kim HB, Reilly CS, Wendt C, Isaacson RE (2012) The lung microbiome in moderate and severe chronic obstructive pulmonary disease. PLoS One 7: 1-10.
    • (2012) PLoS One , vol.7 , pp. 1-10
    • Pragman, A.A.1    Kim, H.B.2    Reilly, C.S.3    Wendt, C.4    Isaacson, R.E.5
  • 23
    • 84893973172 scopus 로고    scopus 로고
    • The lung microbiome. A new frontier in pulmonary medicine
    • Kiley JP, Caler EV (2014) The lung microbiome. A new frontier in pulmonary medicine. Ann Am Thorac Soc 11 Suppl 1: S66-70.
    • (2014) Ann Am Thorac Soc , vol.11 , pp. S66-S70
    • Kiley, J.P.1    Caler, E.V.2
  • 25
    • 0034040183 scopus 로고    scopus 로고
    • Bacterial infection and the pathogenesis of COPD
    • Sethi S (2000) Bacterial infection and the pathogenesis of COPD. Chest 117: 286S-291S.
    • (2000) Chest , vol.117 , pp. 286S-291S
    • Sethi, S.1
  • 26
    • 0037103420 scopus 로고    scopus 로고
    • New strains of bacteria and exacerbations of chronic obstructive pulmonary disease
    • Sethi S, Evans N, Grant BJ, Murphy TF (2002) New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. N Engl J Med 347: 465-471.
    • (2002) N Engl J Med , vol.347 , pp. 465-471
    • Sethi, S.1    Evans, N.2    Grant, B.J.3    Murphy, T.F.4
  • 27
    • 2942624056 scopus 로고    scopus 로고
    • Quantification of intestinal bacterial populations by real-time PCR with a universal primer set and minor groove binder probes: A global approach to the enteric flora
    • Ott SJ, Musfeldt M, Ullmann U, Hampe J, Schreiber S (2004) Quantification of intestinal bacterial populations by real-time PCR with a universal primer set and minor groove binder probes: a global approach to the enteric flora. J Clin Microbiol 42: 2566-2572.
    • (2004) J Clin Microbiol , vol.42 , pp. 2566-2572
    • Ott, S.J.1    Musfeldt, M.2    Ullmann, U.3    Hampe, J.4    Schreiber, S.5
  • 28
    • 84908028519 scopus 로고    scopus 로고
    • Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes
    • Tajadini M, Panjehpour M, Javanmard SH (2014) Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes. Adv Biomed Res 3: 85.
    • (2014) Adv Biomed Res , vol.3 , pp. 85
    • Tajadini, M.1    Panjehpour, M.2    Javanmard, S.H.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.