Performance of amplicon-based next generation DNA sequencing for diagnostic gene mutation profiling in oncopathology

Cellular Oncology

Volumn 37, Issue 5, 2014, Pages 353-361

  Sie, Daoud ^a   Snijders, Peter J F ^a   Meijer, Gerrit A ^a   Doeleman, Marije W ^a   Van Moorsel, Marinda I H ^a   Van Essen, Hendrik F ^a   Eijk, Paul P ^a   Grünberg, Katrien ^a   Van Grieken, Nicole C T ^a   Thunnissen, Erik ^a   Verheul, Henk M ^a   Smit, Egbert F ^a   Ylstra, Bauke ^a   Heideman, Daniëlle A M ^a  

^a VU UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Cancer; FFPE; Molecular pathology; MPS; Somatic mutation; Validation

Indexed keywords

DNA; PARAFFIN; B RAF KINASE; BRAF PROTEIN, HUMAN; EGFR PROTEIN, HUMAN; EPIDERMAL GROWTH FACTOR RECEPTOR; FORMALDEHYDE; KRAS PROTEIN, HUMAN; ONCOPROTEIN; PHOSPHATIDYLINOSITOL 3 KINASE; PIK3CA PROTEIN, HUMAN; RAS PROTEIN; TUMOR MARKER;

AMPLICON; ARTICLE; BRAF GENE; CANCER GENETICS; CANCER PATIENT; CANCER TISSUE; CARCINOGENESIS; COHORT ANALYSIS; CONTROLLED STUDY; DATA ANALYSIS; DNA SEQUENCE; EGFR GENE; GENE SEQUENCE; GENE TECHNOLOGY; GENETIC ALGORITHM; GENETIC ASSOCIATION; GOLD STANDARD; HIGH RESOLUTION MELTING ANALYSIS; HUMAN; HUMAN TISSUE; KRAS GENE; MAJOR CLINICAL STUDY; MOLECULAR DIAGNOSIS; MOLECULAR PATHOLOGY; MUTATIONAL ANALYSIS; PIK3CA GENE; PROSPECTIVE STUDY; RETROSPECTIVE STUDY; SOMATIC MUTATION; TRUSEQ AMPLICON CANCER PANEL MISEQ NEXT GENERATION DNA SEQUENCING; TUMOR GENE; VALIDATION STUDY; CHEMISTRY; GENETICS; HCT116 CELL LINE; HIGH THROUGHPUT SEQUENCING; MUTATION; NEOPLASMS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROCEDURES; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TISSUE FIXATION;

DNA MUTATIONAL ANALYSIS; DNA, NEOPLASM; FORMALDEHYDE; HCT116 CELLS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MUTATION; NEOPLASMS; PARAFFIN EMBEDDING; PHOSPHATIDYLINOSITOL 3-KINASES; POLYMERASE CHAIN REACTION; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS B-RAF; RAS PROTEINS; RECEPTOR, EPIDERMAL GROWTH FACTOR; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TISSUE FIXATION; TUMOR MARKERS, BIOLOGICAL;

EID: 84907761755     PISSN: 22113428     EISSN: 22113436     Source Type: Journal    
DOI: 10.1007/s13402-014-0196-2     Document Type: Article

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