A fast, sensitive and accurate high resolution melting (HRM) technology-based assay to screen for common K-ras mutations

Cellular Oncology

Volumn 31, Issue 3, 2009, Pages 161-167

  Kramer, D ^a   Thunnissen, F B ^a   Gallegos Ruiz, M I ^b   Smit, E F ^b   Postmus, P E ^b   Meijer, C J L M ^a   Snijders, P J F ^a   Heideman, D A M ^a  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

^b ACADEMIC MEDICAL CENTER   (Netherlands)

Author keywords

(nested )PCR; Codon; Direct cycle sequencing; EGFR; Formalin fixed paraffin embedded; G12; G13; Genotype; HRM; K ras; Molecular diagnostics; Receptor tyrosine kinase inhibitors; TKI

Indexed keywords

BIOLOGICAL MARKER; DOUBLE STRANDED DNA; FLUORESCENT DYE; GENOMIC DNA; INTERCALATING AGENT; K RAS PROTEIN;

ACCURACY; ARTICLE; COLORECTAL CANCER; CONTROLLED STUDY; EXON; GENE MUTATION; GENETIC SCREENING; GENOTYPE; HIGH RESOLUTION MELTING POLYMERASE CHAIN REACTION; HOMOZYGOSITY; HUMAN; HUMAN CELL; HUMAN TISSUE; LUNG SMALL CELL CANCER; MELTING POINT; NUCLEOTIDE SEQUENCE; ONCOGENE K RAS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; SENSITIVITY ANALYSIS;

BASE SEQUENCE; CARCINOMA, NON-SMALL-CELL LUNG; DNA MUTATIONAL ANALYSIS; EXONS; GENOTYPE; HUMANS; LUNG NEOPLASMS; MOLECULAR SEQUENCE DATA; MUTATION; ONCOGENE PROTEIN P21(RAS); SENSITIVITY AND SPECIFICITY; TRANSITION TEMPERATURE;

EID: 67650257467     PISSN: 15705870     EISSN: None     Source Type: Journal    
DOI: 10.3233/CLO-2009-0466     Document Type: Article

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