First Evidence of Intraclonal Genetic Exchange in Trypanosomatids Using Two Leishmania infantum Fluorescent Transgenic Clones

PLoS Neglected Tropical Diseases

Volumn 8, Issue 9, 2014, Pages

  Calvo Álvarez, Estefanía ^a   Álvarez Velilla, Raquel ^a   Jiménez, Maribel ^b   Molina, Ricardo ^b   Pérez Pertejo, Yolanda ^a   Balaña Fouce, Rafael ^a   Reguera, Rosa M ^a  

^a UNIVERSITY OF LEÓN   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ARGINASE; FLUORESCENT DYE; NITRIC OXIDE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CLONE; COCULTURE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CULTURE MEDIUM; DNA CONTENT; DNA DETERMINATION; DRUG RESISTANCE; ENZYME ACTIVITY; FEMALE; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC EXCHANGE; GENOTYPE; HISTOPATHOLOGY; HUMAN; HUMAN CELL; HYBRID; IN VITRO STUDY; IN VIVO STUDY; KINETOPLASTID LIFE CYCLE STAGE; LEISHMANIA INFANTUM; LEISHMANIASIS; MACROPHAGE; MICROBIAL GENETICS; MIXED INFECTION; MOUSE; NONHUMAN; PARASITE EXAMINATION; PARASITE LOAD; PARASITE VIRULENCE; PHENOTYPE; PHLEBOTOMUS PERNICIOSUS; POLYMERASE CHAIN REACTION; PROGENY; PROMASTIGOTE; REPORTER GENE; TRANSGENIC ORGANISM; TRIPLOIDY; ANIMAL; BAGG ALBINO MOUSE; DIGESTIVE SYSTEM; FLOW CYTOMETRY; GENETICS; HYBRIDIZATION; LIFE CYCLE STAGE; PARASITOLOGY; PHLEBOTOMUS; PHYSIOLOGY; VISCERAL LEISHMANIASIS;

ANIMALS; DIGESTIVE SYSTEM; FEMALE; FLOW CYTOMETRY; HUMANS; HYBRIDIZATION, GENETIC; LEISHMANIA INFANTUM; LEISHMANIASIS, VISCERAL; LIFE CYCLE STAGES; MICE; MICE, INBRED BALB C; PHLEBOTOMUS;

EID: 84907584462     PISSN: 19352727     EISSN: 19352735     Source Type: Journal    
DOI: 10.1371/journal.pntd.0003075     Document Type: Article

References (40)

1. Matlashewski G, Arana B, Kroeger A, Battacharya S, Sundar S, et al. (2011) Visceral leishmaniasis: elimination with existing interventions. Lancet Infect Dis 11: 322–325.
2. Tibayrenc M, Ben Abderrazak S, Guerrini F, Bañuls A, (1993) Leishmania and the clonal theory of parasitic protozoa. Arch Inst Pasteur Tunis 70: 375–382.
3. Tibayrenc M, Ayala FJ, (2013) How clonal are Trypanosoma and Leishmania? Trends Parasitol 29: 264–249.
4. Rougeron V, De Meeûs T, Hide M, Waleckx E, Bermudez H, et al. (2009) Extreme inbreeding in Leishmania braziliensis. Proc Natl Acad Sci USA 106: 10224–10229.
5. Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, et al. (2011) Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania. Genome Res 21: 2129–2142.
6. Rogers MB, Downing T, Smith BA, Imamura H, Sanders M, et al. (2014) Genomic confirmation of hybridisation and recent inbreeding in a vector-isolated Leishmania population. PLoS Genet 10: e1004092.
7. Mannaert A, Downing T, Imamura H, Dujardin JC, (2012) Adaptive mechanisms in pathogens: universal aneuploidy in Leishmania. Trends Parasitol 28: 370–376.
8. Sterkers Y, Lachaud L, Bourgeois N, Crobu L, Bastien P, et al. (2012) Novel insights into genome plasticity in Eukaryotes: mosaic aneuploidy in Leishmania. Mol Microbiol 86: 15–23.
9. Tibayrenc M, Ayala FJ, (2012) Reproductive clonality of pathogens: a perspective on pathogenic viruses, bacteria, fungi, and parasitic protozoa. Proc Natl Acad Sci U S A 109: E3305–13.
10. Akopyants NS, Kimblin N, Secundino N, Patrick R, Peters N, et al. (2009) Demonstration of genetic exchange during cyclical development of Leishmania in the sand fly vector. Science 324: 265–268.
11. Inbar E, Akopyants NS, Charmoy M, Romano A, Lawyer P, et al. (2013) The mating competence of geographically diverse Leishmania major strains in their natural and unnatural sand fly vectors. PLoS Genet 9: e1003672.
12. Sadlova J, Yeo M, Seblova V, Lewis MD, Mauricio I, et al. (2011) Visualisation of Leishmania donovani fluorescent hybrids during early stage development in the sand fly vector. PLoS One 6: e19851.
13. Bañuls AL, Hide M, Prugnolle F, (2007) Leishmania and the leishmaniases: a parasite genetic update and advances in taxonomy, epidemiology and pathogenicity in humans. Adv Parasitol 64: 1–109.
14. Nolder D, Roncal N, Davies CR, Llanos-Cuentas A, Miles MA, (2007) Multiple hybrid genotypes of Leishmania (Viannia) in a focus of mucocutaneous Leishmaniasis. Am J Trop Med Hyg 76: 573–578.
15. Bonfante-Garrido R, Melendez E, Barroeta S, de Alejos MA, Momen H, et al. (1992) Cutaneous leishmaniasis in western Venezuela caused by infection with Leishmania venezuelensis and L. braziliensis variants. Trans R Soc Trop Med Hyg 86: 141–148.
16. Delgado O, Cupolillo E, Bonfante-Garrido R, Silva S, Belfort E, et al. (1997) Cutaneous leishmaniasis in Venezuela caused by infection with a new hybrid between Leishmania (Viannia) braziliensis and L. (V.) guyanensis. Mem Inst Oswaldo Cruz 92: 581–582.
17. Bañuls AL, Guerrini F, Le Pont F, Barrera C, Espinel I, et al. (1997) Evidence for hybridization by multilocus enzyme electrophoresis and random amplified polymorphic DNA between Leishmania braziliensis and Leishmania panamensis/guyanensis in Ecuador. J Eukaryot Microbiol 44: 408–411.
18. Evans DA, Kennedy WP, Elbihari S, Chapman CJ, Smith V, Peters W, (1987) Hybrid formation within the genus Leishmania? Parassitologia 29: 165–173.
19. Kelly JM, Law JM, Chapman CJ, Van Eys GJ, Evans DA, (1991) Evidence of genetic recombination in Leishmania. Mol Biochem Parasitol 46: 253–263.
20. Ravel C, Cortes S, Pratlong F, Morio F, Dedet JP, et al. (2006) First report of genetic hybrids between two very divergent Leishmania species: Leishmania infantum and Leishmania major. Int J Parasitol 36: 1383–1388.
21. Odiwuor S, De Doncker S, Maes I, Dujardin JC, Van der Auwera G, (2011) Natural Leishmania donovani/Leishmania aethiopica hybrids identified from Ethiopia. Infect Genet Evol 11: 2113–2118.
22. Calvo-Álvarez E, Guerrero NA, Alvarez-Velilla R, Prada CF, Requena JM, et al. (2012) Appraisal of a Leishmania major strain stably expressing mCherry fluorescent protein for both in vitro and in vivo studies of potential drugs and vaccine against cutaneous leishmaniasis. PLoS Negl Trop Dis 6: e1927.
23. Goyard S, Beverley SM, (2000) Blasticidin resistance: a new independent marker for stable transfection of Leishmania. Mol Biochem Parasitol 108: 249–252.
24. Robinson KA, Beverley SM, (2003) Improvements in transfection efficiency and tests of RNA interference (RNAi) approaches in the protozoan parasite Leishmania. Mol Biochem Parasitol 128: 217–228.
25. Sacks DL, Perkins PV, (1984) Identification of an infective stage of Leishmania promastigotes. Science 223: 1417–1419.
26. Titus RG, Marchand M, Boon T, Louis JA, (1985) A limiting dilution assay for quantifying Leishmania major in tissues of infected mice. Parasite Immunol 7: 545–555.
27. Reguera RM, Balaña-Fouce R, Showalter M, Hickerson S, Beverley SM, (2009) Leishmania major lacking arginase (ARG) are auxotrophic for polyamines but retain infectivity to susceptible BALB/c mice. Mol Biochem Parasitol 165: 48–56.
28. Evans TG, Thai L, Granger DL, Hibbs JB, Jr (1993) Effect of in vivo inhibition of nitric oxide production in murine leishmaniasis. J Immunol 151: 907–915.
29. Peacock L, Ferris V, Bailey M, Gibson W, (2009) Intraclonal mating occurs during tsetse transmission of Trypanosoma brucei. Parasit Vectors 2: 43.
30. Gibson W, Garside L, Bailey M, (1992) Trisomy and chromosome size changes in hybrid trypanosomes from a genetic cross between Trypanosoma brucei rhodesiense and T. b. brucei. Mol Biochem Parasitol 51: 189–199.
31. Gibson W, Peacock L, Ferris V, Williams K, Bailey M, (2008) The use of yellow fluorescent hybrids to indicate mating in Trypanosoma brucei. Parasit Vectors 1: 4.
32. Hope M, MacLeod A, Leech V, Melville V, Sasse J, et al. (1999) Analysis of ploidy (in megabase chromosomes) in Trypanosoma brucei after genetic exchange. Mol Biochem Parasitol 104: 1–9.
33. Cruz AK, Titus R, Beverley SM, (1993) Plasticity in chromosome number and testing of essential genes in Leishmania by targeting. Proc Natl Acad Sci USA 90: 1599–1603.
34. Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, et al. (2005) The genome of the kinetoplastid parasite, Leishmania major. Science 309: 436–442.
35. Heitman J, (2010) Evolution of eukaryotic microbial pathogens via covert sexual reproduction. Cell Host Microbe 8: 86–99.
36. MacLeod A, Tweedie A, McLellan S, Hope M, Taylor S, et al. (2005) Allelic segregation and independent assortment in T. brucei crosses: Proof that the genetic system is Mendelian and involves meiosis. Mol Biochem Parasitol 143: 12–19.
37. Peacock L, Ferris V, Sharma R, Sunter J, Bailey M, et al. (2011) Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly. Proc Natl Acad Sci USA 108: 3671–3676.
38. Peacock L, Bailey M, Carrington M, Gibson W, (2014) Meiosis and haploid gametes in the pathogen Trypanosoma brucei. Curr Biol 24: 181–186.
39. Abebe T, Hailu A, Woldeyes M, Mekonen W, Bilcha K, et al. (2012) Local increase of arginase activity in lesions of patients with cutaneous leishmaniasis in Ethiopia. PLoS Negl Trop Dis 6: e1684.
40. Ibrahim AS, Magee BB, Sheppard DC, Yang M, Kauffman S, et al. (2005) Effects of ploidy and mating type on virulence of Candida albicans. Infect Immun 73: 7366–7374.