Use of genomics to identify bacterial undecaprenyl pyrophosphate synthetase: Cloning, expression, and characterization of the essential uppS gene

Journal of Bacteriology

Volumn 181, Issue 2, 1999, Pages 483-492

  Apfel, Christian M ^a   Takács, Béla ^a   Fountoulakis, Michael ^a   Stieger, Martin ^a   Keck, Wolfgang ^a  

^a F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

(2 DIAZO 3 TRIFLUOROPROPIONYLOXY)GERANYLDIPHOSPHATE; BACTERIAL DNA; BACTERIAL ENZYME; DECAPRENYLTRANSFERASE; DIMETHYLALLYLTRANSFERASE; ETHER; HEPARIN; HYDROXYAPATITE; MAGNESIUM CHLORIDE; PYROPHOSPHORIC ACID DERIVATIVE; TRITON X 100; TRYPSIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; HAEMOPHILUS INFLUENZAE; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; STREPTOCOCCUS PNEUMONIAE;

BACTERIA (MICROORGANISMS); CAENORHABDITIS ELEGANS; ESCHERICHIA COLI; HAEMOPHILUS INFLUENZAE; NEGIBACTERIA; SACCHAROMYCES CEREVISIAE; STREPTOCOCCUS PNEUMONIAE;

EID: 0345313624     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.2.483-492.1999     Document Type: Article

References (40)

1. Adair, W. L., N. Cafmeyer, and R. K. Keller. 1984. Solubilization and characterization of the long chain prenyltransferases involved in dolichyl phosphate biosynthesis. J. Biol Chem. 259:4441-4446.
2. Allen, C. M., M. V. Keenan, and J. Sack. 1976. Lactobacillus plantarum undecaprenyl pyrophosphate synthetase: purification and reaction requirements. Arch. Biochem. Biophys. 175:236-248.
3. Allen, C. M., and J. D. Muth. 1977. Lipid activation of undecaprenyl pyrophosphate synthetase from Lactobacillus plantarum. Biochemistry 16:2908-2915.
4. Allen, C. M. 1985. Purification and characterization of undecaprenyl-pyrophosphate synthetase. Methods Enzymol. 110:281-299.
5. Asai, K., S. Fujisaki, Y. Nishimura, T. Nishino, K. Okada, T. Nakagawa, M. Kawamukai, and H. Matsuda. 1994. The identification of E. coli ispB (cel) gene encoding the octaprenyl diphosphate synthase. Biochem. Biophys. Res. Commun. 202:340-345.
6. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.), 1997. Current protocols in molecular biology. Wiley Interscience, New York, N.Y.
7. Avery, O. T., C. M. MacLeod, and M. McCarty. 1944. Studies on the chemical nature of the substance inducing transformation of pneumococcal types. J. Exp. Med. 79:137-158.
8. Baba, T., and C. M. Allen. 1978. Substrate specificity of undecaprenyl pyrophosphate synthetase from Lactobacillus plantarum. Biochemistry 17:5598-5604.
9. Baba, T., and C. M. Allen. 1980. Prenyl transferases from Micrococcus luteus: characterization of undecaprenyl pyrophosphate synthetase. Arch Biochem. Biophys. 200:474-484.
10. Baba, T., J. Muth, and C. M. Allen. 1985. Photoaffinity labeling of undecaprenyl pyrophosphate synthetase with a farnesyl pyrophosphate analogue. J. Biol. Them. 260:10467-10473.
11. Bukhtiyarov, Y. E., Y. A. Shabalin, and I. S. Kulaev. 1993. Solubilization and characterization of dehydrodolichyl diphosphate synthase from the yeast Saccharomyces carlbergensis. J. Biochem. 113:721-728.
12. Chen, J. D., and D. A. Morrison. 1988. Construction and properties of a new insertion vector, pJDC9, that is protected by transcriptional terminators and useful for cloning of DNA of Streptococcus pneumoniae. Gene 64:155-154.
13. Christenson, J. G., S. K. Gross, and P. W. Robbins. 1961. Enzymatic synthesis of the antigen carrier lipid. J. Biol. Chem. 244:5436-5439.
14. Collins, M. D., and D. Jones. 1981. Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implementations. Microbiol. Rev. 45:316-354.
15. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
16. Fountoulakis, M., and H. Langen. 1997. Identification of proteins by matrix-assisted laser desorption ionization-mass spectromelry following in-gel digestion in low-salt, nonvolatile buffer and simplified peptide recovery. Anal. Biochem. 250:153-156.
17. Fujisaki, S., T. Nishino, and H. Katsuki. 1986. Isoprenoid synthesis in Escherichia coli. Separation and partial purification of four enzymes involved in the synthesis. J. Biochem. 99:1327-1337.
18. Fujisaki, S., H. Hara, Y. Nishimura, K. Horiuchi, and T. Nishino. 1990. Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in E. coli. J. Biochem. 108:995-1000.
19. Havarstein, L. S., G. Coomaraswamy, and D. A. Morrison. 1995. An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae. Proc. Natl. Acad. Sci. USA 92: 11140-11144.
20. Keenan, M. V., and C. M. Allen. 1974. Characterization of undecaprenyl pyrophosphate synthetase from Lactobacillus plantarum. Arch. Biochem. Biophys. 161:375-383.
21. Kodama, T., K. Fukui, and K. Kometani. 1986. The initial phosphate burst in ATP hydrolysis by myosin and subfragment-1 as studied by a modified malachite green method for determination of inorganic phosphate. J. Biochem. 99:1465-1472.
22. Kurokawa, T., K. Ogura, and S. Seto. 1971. Formation of polyprenyl phosphates by a cell-free enzyme of Micrococcus lysodeikticus. Biochem. Biophys. Res. Commun. 45:251-257.
23. Lacks, S. 1966. Integration efficiency and genetic recombination in pneumococcal transformation. Genetics 53:207-235.
24. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bactcriophage T4. Nature 227:680-685.
25. Lesburg, C. A., G. Zhai, D. E. Cane, and D. W. Christianson. 1997. Crystal structure of pentalenene synthase: mechanistic insights on terpenoid cyclization reactions in biology. Science 277:1820-1824.
26. 3H]-(2-diazo-3-trifluoropropionyloxy)geranyl diphosphate. J. Labelled Compd. Radiopharm. 38:139-148.
27. Matsuoka, S., Sagami, H., Kurisaki, A., and K. Ogura. 1991. Variable product specificity of microsomal dehydrodolichyl diphosphate synthase from rat liver. J. Biol. Chem. 266:3464-3668.
28. Ogura, K., T. Koyama, and H. Sagami. 1997. Polyprenyl diphosphate synthases. Subcell. Biochem. 28:57-87.
29. Pozzi, G., M. R. Oggioni, R. Manganelli, and P. Plevani. 1991. Genetic manipulation of streptococci by chromosomal integration of recombinant DNA, p. 59-61. In G. M. Dunny, P. P. Cleary, and L. L. McKay (ed.), Genetics and molecular biology of streptococci, lactococci, and enterococci. Washington, D.C.
30. Sambrook, R., E. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
31. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 71:5463-5467.
32. Shimizu, N., T. Koyama, and K. Ogura. 1998. Molecular cloning, expression, and purification of undecaprenyl diphosphate synthase: no sequence similarity between E-and Z-prenyl diphosphate synthases. J. Biol. Chem. 273: 19476-19481.
33. Starks, C. M., K. Back, J. Chappell, and J. P. Noel. 1997. Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase. Science 277:1815-1820.
34. Stieger, M., B. Wohlgensinger, M. Kamber, R. Lutz, and W. Keck. Integrational plasmids for the tetracycline regulated expression of genes in Streptococcus pneumoniae. Gene, in press.
35. Takahashi, I., and K. Ogura. 1982. Prenyltransferase of Bacillus subtilis: undecaprenyl pyrophosphate synthetase and geranylgeranyl pyrophosphate synthetase. J. Biochem. 92:1527-1537.
36. Takahashi, S., T. Kuzuyama, H. Watanabe, and H. Seto. 1998. A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis. Proc. Natl. Acad. Sci. USA 95:9879-9884.
37. Tarshis, L. C., P. J. Proteau, B. A. Kellogg, J. C. Sacchettini, and C. D. Poulter. 1996. Regulation of product chain length by isoprenyl diphosphate synthases. Proc. Natl. Acad. Sci. USA 93:15018-150223.
38. Umbreit, J. N., and J. L. Strominger. 1972. Isolation of the lipid intermediate in peptidoglycan biosynthesis from Escherichia coli. J. Bacteriol. 122:1306-1309.
39. van Heijenoort, V., M. Gómez, M. Derrien, J. Ayala, and J. van Heijenoort. 1992. Membrane intermediates in the peptidoglycan metabolism of Escherichia coli: possible roles of PBP 1b and PBP 3. J. Bacteriol. 174:3549-3557.
40. Wendt, K. U., K. Poralla, and G. E. Schulz. 1997. Structure and function of a squalene cyclase. Science 277:1811-1815.