Disruption of glucocorticoid signaling in chondrocytes delays metaphyseal fracture healing but does not affect normal cartilage and bone development

Bone

Volumn 69, Issue , 2014, Pages 12-22

  Tu, Jinwen ^a   Henneicke, Holger ^a   Zhang, Yaqing ^a   Stoner, Shihani ^a   Cheng, Tegan L ^b   Schindeler, Aaron ^b   Chen, Di ^c   Tuckermann, Jan ^d   Cooper, Mark S ^e   Seibel, Markus J ^a,e   Zhou, Hong ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

^b CHILDREN S HOSPITAL AT WESTMEAD   (Australia)

^c RUSH UNIVERSITY MEDICAL CENTER   (United States)

^d UNIVERSITY OF ULM   (Germany)

^e ANZAC Research Institute   (Australia)

Author keywords

Cartilage; Chondrocyte; Diaphyseal fracture; Glucocorticoids; Growth; Metaphyseal fracture

Indexed keywords

GLUCOCORTICOID; GLUCOCORTICOID RECEPTOR;

ADULT; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BONE DENSITY; BONE DEVELOPMENT; BONE GROWTH; BONE LENGTH; BONE MICRO ARCHITECTURE; BONE MINERALIZATION; BONE STRENGTH; BONE STRUCTURE; CALLUS; CARTILAGE CELL; CARTILAGE STRUCTURE; CELL FUNCTION; CHONDROGENESIS; CONTROLLED STUDY; DIAPHYSEAL FRACTURE; DIAPHYSIS; FEMALE; FRACTURE; FRACTURE NONUNION; GROWTH PLATE; HORMONE ACTION; IN VIVO STUDY; INTERVERTEBRAL DISK; INTRACELLULAR SIGNALING; KNEE JOINT ARCHITECTURE; LONG BONE; MALE; METAPHYSEAL FRACTURE; METAPHYSIS; MOUSE; MUSCULOSKELETAL SYSTEM PARAMETERS; NONHUMAN; PERINATAL PERIOD; POSTNATAL DEVELOPMENT; YOUNG ADULT; ANIMAL; C57BL MOUSE; CARTILAGE; FRACTURE HEALING; GROWTH, DEVELOPMENT AND AGING; IMMUNOHISTOCHEMISTRY; KNOCKOUT MOUSE; METABOLISM; PHYSIOLOGY; SIGNAL TRANSDUCTION;

ANIMALS; BONE DEVELOPMENT; CARTILAGE; CHONDROCYTES; FRACTURE HEALING; GLUCOCORTICOIDS; IMMUNOHISTOCHEMISTRY; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; SIGNAL TRANSDUCTION;

EID: 84907525576     PISSN: 87563282     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bone.2014.08.016     Document Type: Article

References (40)

1. Barnes P.J., Adcock I.M. Glucocorticoid resistance in inflammatory diseases. Lancet 2009, 373(9678):1905-1917.
2. Hench P.S. Cortisone, hydrocortisone and corticotropin; some facts and speculations with special reference to rheumatoid arthritis. Trans Assoc Life Insur Med Dir Am 1951, 35:5-33.
3. Hardy R., Cooper M.S. Glucocorticoid-induced osteoporosis-a disorder of mesenchymal stromal cells?. Front Endocrinol (Lausanne) 2011, 2:24.
4. Sorva R.A., Turpeinen M.T. Asthma, glucocorticoids and growth. Ann Med 1994, 26(4):309-314.
5. Allen D.B. Growth suppression by glucocorticoid therapy. Endocrinol Metab Clin North Am 1996, 25(3):699-717.
6. Vapra A., Kanmer K. [Pathologic bone fractures in patients treated with corticosteroid preparations for long periods of time]. Vopr Revm 1967, 7(3):80-82.
7. Luppen C.A., Blake C.A., Ammirati K.M., Stevens M.L, Seeherman H.J, Wozney J.M, Bouxsein M.L., et al. Recombinant human bone morphogenetic protein-2 enhances osteotomy healing in glucocorticoid-treated rabbits. J Bone Miner Res 2002, 17(2):301-310.
8. Doyon A.R., Ferries I.K., Li J. Glucocorticoid attenuates the anabolic effects of parathyroid hormone on fracture repair. Calcif Tissue Int 2010, 87(1):68-76.
9. Kronenbe H.M. Developmental regulation of the growth plate. Nature 2003, 423(6937):332-336.
10. Einhorn T.A. The science of fracture healing. J Orthop Trauma 2005, 19(10 Suppl.):S4-S6.
11. Webb J.C.J. A review of fracture healing. Curr Orthop 2000, 14:457-463.
12. Zhou H., Mak W., Kalak R., Street J., Fong-Yee C., Zheng Y., et al. Glucocorticoid-dependent Wnt signaling by mature osteoblasts is a key regulator of cranial skeletal development in mice. Development 2009, 136(3):427-436.
13. Zhou H., Mak W., Zheng Y., Dunstan C.R., Seibel M.J. Osteoblasts directly control lineage commitment of mesenchymal progenitor cells through Wnt signaling. J Biol Chem 2008, 283(4):1936-1945.
14. Weber A.J., Li G., Kalak R., Street J., Buttgereit F., Dunstan C.R., et al. Osteoblast-targeted disruption of glucocorticoid signalling does not delay intramembranous bone healing. Steroids 2010, 75(3):282-286.
15. Buttgereit F., Zhou H., Kalak R., Gaber T., Spies C.M., Huscher D., et al. Transgenic disruption of glucocorticoid signaling in mature osteoblasts and osteocytes attenuates K/BxN mouse serum-induced arthritis in vivo. Arthritis Rheum 2009, 60(7):1998-2007.
16. Rauch A., Seitz S., Baschant U., Schilling A.F., Illing A., Stride B., et al. Glucocorticoids suppress bone formation by attenuating osteoblast differentiation via the monomeric glucocorticoid receptor. Cell Metab 2010, 11(6):517-531.
17. Silvestrini G., Mocetti P., Di Grezia R., Berni S., Bonucci E. Localization of the glucocorticoid receptor mRNA in cartilage and bone cells of the rat. An in situ hybridization study. Eur J Histochem 2003, 47(3):245-252.
18. James C.G., Appleton C.T., Ulici V., Underhill T.M., Beier F. Microarray analyses of gene expression during chondrocyte differentiation identifies novel regulators of hypertrophy. Mol Biol Cell 2005, 16(11):5316-5333.
19. James C.G., Ulici V., Tuckermann J., Underhill T.M., Beier F. Expression profiling of dexamethasone-treated primary chondrocytes identifies targets of glucocorticoid signalling in endochondral bone development. BMC Genomics 2007, 8:205.
20. Chen M., Lichtler A.C., Sheu T.J., Xie C., Zhang X., O'Keefe R.J., et al. Generation of a transgenic mouse model with chondrocyte-specific and tamoxifen-inducible expression of Cre recombinase. Genesis 2007, 45(1):44-50.
21. Zhu M., Chen M., Lichtler A.C., O'Keefe R.J., Chen D. Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1-CreER(T2) transgenic mice. Osteoarthritis Cartilage 2008, 16(1):129-130.
22. Zhu M., Tang D., Wu Q., Hao S., Chen M., Xie C., et al. Activation of beta-catenin signaling in articular chondrocytes leads to osteoarthritis-like phenotype in adult beta-catenin conditional activation mice. J Bone Miner Res 2009, 24(1):12-21.
23. Tronche F., Kellendonk C., Kretz O., Gass P., Anlag K., Orban P.C., et al. Disruption of the glucocorticoid receptor gene in the nervous system results in reduced anxiety. Nat Genet 1999, 23(1):99-103.
24. Li A., Hardy R., Stoner S., Tuckermann J., Seibel M., Zhou H. Deletion of mesenchymal glucocorticoid receptor attenuates embryonic lung development and abdominal wall closure. PLoS One 2013, 8(5):e63578.
25. Mao X., Fujiwara Y., Orkin S.H. Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice. Proc Natl Acad Sci U S A 1999, 96(9):5037-5042.
26. Xie C., Xue M., Wang Q., Schwarz E.M., O'Keefe R.J., Zhang X. Tamoxifen-inducible CreER-mediated gene targeting in periosteum via bone-graft transplantation. J Bone Joint Surg Am 2008, 90(Suppl. 1):9-13.
27. Henneicke H., Herrmann M., Kalak R., Brennan-Speranza T.C., Heinevetter U., Bertollo N., et al. Corticosterone selectively targets endo-cortical surfaces by an osteoblast-dependent mechanism. Bone 2011, 49(4):733-742.
28. Seeman E., Delmas P.D. Bone quality-the material and structural basis of bone strength and fragility. N Engl J Med 2006, 354(21):2250-2261.
29. Fajardo R.J., Cory E., Patel N.D., Nazarian A., Laib A., Manoharan R.K., et al. Specimen size and porosity can introduce error into microCT-based tissue mineral density measurements. Bone 2009, 44(1):176-184.
30. Bouxsein M.L., Boyd S.K., Christiansen B.A., Guldberg R.E., Jepsen K.J., Muller R. Guidelines for assessment of bone microstructure in rodents using micro-computed tomography. J Bone Miner Res 2010, 25(7):1468-1486.
31. Lukas C., Ruffoni D., Lambers F.M., Schulte F.A., Kuhn G., Kollmannsberger P., et al. Mineralization kinetics in murine trabecular bone quantified by time-lapsed in vivo micro-computed tomography. Bone 2013, 56(1):55-60.
32. Cole T.J., Blendy J.A., Monaghan A.P., Krieglstein K., Schmid W., Aguzzi A., et al. Targeted disruption of the glucocorticoid receptor gene blocks adrenergic chromaffin cell development and severely retards lung maturation. Genes Dev 1995, 9(13):1608-1621.
33. Waters R.V., Gamradt S.C., Asnis P., Vickery B.H., Avnur Z., Hill E., et al. Systemic corticosteroids inhibit bone healing in a rabbit ulnar osteotomy model. Acta Orthop Scand 2000, 71(3):316-321.
34. Bostrom M.P., Gamradt S.C., Asnis P., Vickery B.H., Hill E., Avnur E., et al. Parathyroid hormone-related protein analog RS-66271 is an effective therapy for impaired bone healing in rabbits on corticosteroid therapy. Bone 2000, 26(5):437-442.
35. Beaupied H., Lespessailles E., Benhamou C.L. Evaluation of macrostructural bone biomechanics. Joint Bone Spine 2007, 74(3):233-239.
36. Murnaghan M., McIlmurray L., Mushipe M.T., Li G. Time for treating bone fracture using rhBMP-2: a randomised placebo controlled mouse fracture trial. J Orthop Res 2005, 23(3):625-631.
37. Wang W., Nyman J.S., Moss H.E., Gutierrez G., Mundy G.R., Yang X., et al. Local low-dose lovastatin delivery improves the bone-healing defect caused by Nf1 loss of function in osteoblasts. J Bone Miner Res 2010, 25(7):1658-1667.
38. Konstantinidis I., Papageorgiou S.N., Kyrgidis A., Tzellos T.G., Kouvelas D. Effect of non-steroidal anti-inflammatory drugs on bone turnover: an evidence-based review. Rev Recent Clin Trials 2013, 8(1):48-60.
39. Granero-Molto F., Weis J.A., Miga M.I., Landis B., Myers T.J., O'Rear L., et al. Regenerative effects of transplanted mesenchymal stem cells in fracture healing. Stem Cells 2009, 27(8):1887-1898.
40. Raza K., Hardy R., Cooper M.S. The 11beta-hydroxysteroid dehydrogenase enzymes-arbiters of the effects of glucocorticoids in synovium and bone. Rheumatology (Oxford) 2010, 49(11):2016-2023.