Comparing the identification of Clostridium spp. by two Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) mass spectrometry platforms to 16S rRNA PCR sequencing as a reference standard: A detailed analysis of age of culture and sample preparation

Anaerobe

Volumn 30, Issue , 2014, Pages 85-89

  Chean, Roy ^a   Kotsanas, Despina ^b   Francis, Michelle J ^b   Palombo, Enzo A ^c   Jadhav, Snehal R ^c   Awad, Milena M ^d   Lyras, Dena ^d   Korman, Tony M ^b,d   Jenkin, Grant A ^b  

^a ROYAL MELBOURNE HOSPITAL   (Australia)

^b SOUTHERN HEALTH   (Australia)

^c SWINBURNE UNIVERSITY OF TECHNOLOGY   (Australia)

^d MONASH UNIVERSITY   (Australia)

Author keywords

Age of culture; Clostridium; MALDI TOF; Nucleic acid sequencing; Sample preparation

Indexed keywords

RNA 16S; BACTERIAL DNA; RIBOSOME DNA;

AGE; ANALYTIC METHOD; ARTICLE; BACTERIAL GROWTH; BACTERIUM COLONY; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BRUKER MASS SPECTROMETER; CLOSTRIDIUM; CLOSTRIDIUM PERFRINGENS; CONTROLLED STUDY; DIRECT TRANSFER; EXTENDED DIRECT TRANSFER; FULL FORMIC ACID EXTRACTION; MASS SPECTROMETER; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; POLYMERASE CHAIN REACTION; RNA SEQUENCE; SPECIES DIFFERENTIATION; VITEK MASS SPECTROMETER; BACTEREMIA; CHEMISTRY; CLASSIFICATION; CLOSTRIDIUM INFECTIONS; CLUSTER ANALYSIS; COMPARATIVE STUDY; DNA SEQUENCE; EVALUATION STUDY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; MASS SPECTROMETRY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MOLECULAR GENETICS; PHYLOGENY; PROCEDURES; STANDARD;

BACTEREMIA; BACTERIOLOGICAL TECHNIQUES; CLOSTRIDIUM; CLOSTRIDIUM INFECTIONS; CLUSTER ANALYSIS; DNA, BACTERIAL; DNA, RIBOSOMAL; HUMANS; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SPECIMEN HANDLING; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84907483706     PISSN: 10759964     EISSN: 10958274     Source Type: Journal    
DOI: 10.1016/j.anaerobe.2014.09.007     Document Type: Article

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