Species identification of clinical isolates of anaerobic bacteria: A comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems

Journal of Clinical Microbiology

Volumn 49, Issue 12, 2011, Pages 4314-4318

  Justesen, Ulrik Stenz ^a   Holm, Anette ^b   Knudsen, Elisa ^a   Andersen, Line Bisgaard ^b   Jensen, Thøger Gorm ^a   Kemp, Michael ^a   Skov, Marianne Nielsine ^a   Gahrn Hansen, Bente ^a   Møller, Jens Kjølseth ^b  

^a ODENSE UNIVERSITY HOSPITAL   (Denmark)

^b LILLEBAELT HOSPITAL   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ACTINOMYCES; ANAEROBIC BACTERIUM; ANALYTICAL ERROR; ARTICLE; BACTERIUM CULTURE; BACTERIUM ISOLATE; BACTEROIDES FRAGILIS; BACTEROIDES THETAIOTAOMICRON; BACTEROIDES VULGATUS; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM PERFRINGENS; DATA BASE; FUSOBACTERIUM NECROPHORUM; GENE IDENTIFICATION; GENE SEQUENCE; GOLD STANDARD; INTERMETHOD COMPARISON; LACTOBACILLUS; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PEPTOSTREPTOCOCCUS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROPIONIBACTERIUM ACNES; SEQUENCE ANALYSIS; SPECIES IDENTIFICATION;

BACTERIA, ANAEROBIC; BACTERIAL INFECTIONS; BACTERIOLOGICAL TECHNIQUES; HUMANS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS;

EID: 82455162798     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.05788-11     Document Type: Article

References (19)

1. Bizzini, A., C. Durussel, J. Bille, G. Greub, and G. Prod'hom. 2010. Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory. J. Clin. Microbiol. 48:1549-1554.
2. Bizzini, A., and G. Greub. 2010. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. Clin. Microbiol. Infect. 16:1614-1619.
3. Cherkaoui, A., et al. 2010. Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. J. Clin. Microbiol. 48:1169-1175.
4. Chun, J., et al. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57:2259-2261. (Pubitemid 350031697)
5. Citron, D. M., and P. C. Appelbaum. 1993. How far should a clinical laboratory go in identifying anaerobic isolates, and who should pay? Clin. Infect. Dis. 16(Suppl. 4):S435-S438. (Pubitemid 23157968)
6. Clinical and Laboratory Standards Institute. 2008. Interpretive criteria for identification of bacteria and fungi by DNA target sequencing. Approved standard MM18-A, 1st ed. Clinical and Laboratory Standards Institute, Wayne, PA.
7. Downes, J., A. King, J. Hardie, and I. Phillips. 1999. Evaluation of the Rapid ID 32A system for identification of anaerobic Gram-negative bacilli, excluding the Bacteroides fragilis group. Clin. Microbiol. Infect. 5:319-326. (Pubitemid 29312609)
8. Justesen, U. S., et al. 2010. 16S rRNA gene sequencing in routine identification of anaerobic bacteria isolated from blood cultures. J. Clin. Microbiol. 48:946-948.
9. Justesen, U. S., et al. 2011. Preliminary results from a comparison of two matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry (MALDI-TOF) systems with 16S rRNA gene sequencing for identification of anaerobic bacteria to the species level. Clin. Microbiol. Infect. 17(Suppl. 4):S66.
10. Justesen, U. S., E. Matuschek, and G. Kahlmeter. 2011. The EUCAST Mueller-Hinton fastidious agar for antimicrobial susceptibility testing of anaerobic bacteria? Clin. Microbiol. Infect. 17(Suppl. 4):S174.
11. Lee, E. H., J. E. Degener, G. W. Welling, and A. C. Veloo. 2011. Evaluation of the Vitek 2 ANC card for identification of clinical isolates of anaerobic bacteria. J. Clin. Microbiol. 49:1745-1749.
12. Lomholt, H. B., and M. Kilian. 2010. Population genetic analysis of Propionibacterium acnes identifies a subpopulation and epidemic clones associated with acne. PLoS One 5:e12277.
13. Nagy, E., T. Maier, E. Urban, G. Terhes, and M. Kostrzewa for the ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria. 2009. Species identification of clinical isolates of Bacteroides by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. Clin. Microbiol. Infect. 15:796-802.
14. Nassif, X. 2009. A revolution in the identification of pathogens in clinical laboratories. Clin. Infect. Dis. 49:552-553.
15. Seng, P., et al. 2009. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin. Infect. Dis. 49:543-551.
16. van Veen, S. Q., E. C. Claas, and E. J. Kuijper. 2010. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization- time of flight mass spectrometry in conventional medical microbiology laboratories. J. Clin. Microbiol. 48:900-907.
17. Veloo, A. C., M. Erhard, M. Welker, G. W. Welling, and J. E. Degener. 2011. Identification of Gram-positive anaerobic cocci by MALDI-TOF mass spectrometry. Syst. Appl. Microbiol. 34:58-62.
18. Veloo, A. C., M. Knoester, J. E. Degener, and E. J. Kuijper. 2011. Comparison of two matrix-assisted laser desorption ionisation-time of flight mass spectrometry methods for the identification of clinically relevant anaerobic bacteria. Clin. Microbiol. Infect. 17:1501-1506.
19. Veloo, A. C., G. W. Welling, and J. E. Degener. 2011. The identification of anaerobic bacteria using MALDI-TOF MS. Anaerobe 17:211-212.