Comparison of two matrix-assisted laser desorption ionisation-time of flight mass spectrometry methods for the identification of clinically relevant anaerobic bacteria

Clinical Microbiology and Infection

Volumn 17, Issue 10, 2011, Pages 1501-1506

  Veloo, A C M ^a   Knoester, M ^b   Degener, J E ^a   Kuijper, E J ^b  

^a UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Anaerobic; Bacteria; Identification; MALDI TOF; Mass spectrometry

Indexed keywords

RNA 16S;

ACTINOMYCES; AEROCOCCUS; ANAEROBIC BACTERIUM; ARTICLE; ATOPOBIUM; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BACTEROIDES FRAGILIS; BILOPHILA; CAMPYLOBACTER; CLOSTRIDIUM; EGGERTHELLA; FINEGOLDIA; FUSOBACTERIUM; GENE IDENTIFICATION; GENE SEQUENCE; GRAM POSITIVE COCCI; INTERMETHOD COMPARISON; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PARABACTEROIDES; PARVIMONAS; PEPTOCOCCUS; PEPTONIPHILUS; PEPTOSTREPTOCOCCUS; PORPHYROMONAS; PREVOTELLA; PRIORITY JOURNAL; PROPIONIBACTERIUM; VEILLONELLA;

BACTEROIDES FRAGILIS; POSIBACTERIA;

EID: 80053082459     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2011.03467.x     Document Type: Article

References (12)

1. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. Anaerobic bacteriology manual, 6th edn. Belmont, CA: Star Publishing Company, 2002.
2. Wildeboer-Veloo AC, Harmsen HJ, Welling GW, Degener JE. Development of 16S rRNA-based probes for the identification of Gram-positive anaerobic cocci isolated from human clinical specimens. Clin Microbiol Infect 2007; 13: 985-992.
3. Song Y. PCR-based diagnostics for anaerobic infections. Anaerobe 2005; 11: 79-91.
4. Grosse-Herrenthey A, Maier T, Gessler F et al. Challenging the problem of clostridial identification with matrix-assisted laser desorption and ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Anaerobe 2008; 14: 242-249.
5. Nagy E, Maier T, Urban E, Terhes G, Kostrzewa M. Species identification of clinical isolates of Bacteroides by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. Clin Microbiol Infect 2009; 15: 796-802.
6. Stingu CS, Rodloff AC, Jentsch H, Schaumann R, Eschrich K. Rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by MALDI-TOF-MS. Oral Microbiol Immunol 2008; 23: 372-376.
7. Cherkaoui A, Hibbs J, Emonet S et al. Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. J Clin Microbiol 2010; 48: 1169-1175.
8. Seng P, Drancourt M, Gouriet F et al. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 2009; 49: 543-551.
9. van Veen SQ, Claas EC, Kuijper EJ. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories. J Clin Microbiol 2010; 48: 900-907.
10. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990; 28: 495-503.
11. Hiraishi A. Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 1992; 15: 210-213.
12. Keys CJ, Dare DJ, Sutton H et al. Compilation of a MALDI-TOF mass spectral database for the rapid screening and characterisation of bacteria implicated in human infectious diseases. Infect Genet Evol 2004; 4: 221-242.