Multicenter evaluation of the vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of gram-positive aerobic bacteria

Journal of Clinical Microbiology

Volumn 51, Issue 7, 2013, Pages 2225-2231

  Rychert, Jenna ^a   Burnham, Carey Ann D ^b   Bythrow, Maureen ^d   Garner, Omai B ^e   Ginocchio, Christine C ^f   Jennemann, Rebecca ^c   Lewinski, Michael A ^e   Manji, Ryhana ^d   Mochon, A Brian ^e   Procop, Gary W ^g   Richter, Sandra S ^g   Sercia, Linda ^g   Westblade, Lars F ^b,f   Ferraro, Mary Jane ^a   Branda, John A ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c BARNES JEWISH HOSPITAL   (United States)

^d North Shore LIJ Health System Laboratories ^*  (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

^f DONALD AND BARBARA ZUCKER SCHOOL OF MEDICINE AT HOFSTRA NORTHWELL   (United States)

^g LERNER RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURACY; ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATE; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; ENTEROCOCCUS; GARDNERELLA VAGINALIS; GENUS; GRAM POSITIVE BACTERIUM; LEUCONOSTOC MESENTEROIDES; LISTERIA MONOCYTOGENES; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROCOCCUS LUTEUS; NONHUMAN; PRIORITY JOURNAL; ROTHIA MUCILAGINOSA; STREPTOCOCCUS; STREPTOCOCCUS DYSGALACTIAE; STREPTOCOCCUS MITIS; STREPTOCOCCUS ORALIS; STREPTOCOCCUS PNEUMONIAE; AEROBIC BACTERIUM; CHEMISTRY; CLASSIFICATION; CLINICAL TRIAL; COMPARATIVE STUDY; DIAGNOSTIC ERROR; EVALUATION STUDY; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; MICROBIOLOGICAL EXAMINATION; MULTICENTER STUDY; PROCEDURES; SENSITIVITY AND SPECIFICITY; STATISTICS AND NUMERICAL DATA;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STREPTOCOCCUS PNEUMONIAE;

BACTERIA, AEROBIC; BACTERIOLOGICAL TECHNIQUES; DIAGNOSTIC ERRORS; GRAM-POSITIVE BACTERIA; HUMANS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84879429052     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00682-13     Document Type: Article

References (44)

1. Ruoff K. 2011. General approaches to identification of aerobic grampositive cocci, p 304-307. In Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW (ed), Manual of clinical microbiology, 10th ed, vol 1. ASM Press, Washington DC.
2. Neville SA, Lecordier A, Ziochos H, Chater MJ, Gosbell IB, Maley MW, van Hal SJ. 2011. Utility of matrix-assisted laser desorption ionizationtime of flight mass spectrometry following introduction for routine laboratory bacterial identification. J. Clin. Microbiol. 49:2980-2984.
3. Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D. 2009. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-offlight mass spectrometry. Clin. Infect. Dis. 49:543-551.
4. Eigner U, Holfelder M, Oberdorfer K, Betz-Wild U, Bertsch D, Fahr AM. 2009. Performance of a matrix-assisted laser desorption ionizationtime-of-flight mass spectrometry system for the identification of bacterial isolates in the clinical routine laboratory. Clin. Lab. 55:289-296.
5. El-Bouri K, Johnston S, Rees E, Thomas I, Bome-Mannathoko N, Jones C, Reid M, Ben-Ismaeil B, Davies AR, Harris LG, Mack D. 2012. Comparison of bacterial identification by MALDI-TOF mass spectrometry and conventional diagnostic microbiology methods: agreement, speed and cost implications. Br. J. Biomed. Sci. 69:47-55.
6. Martiny D, Busson L, Wybo I, El Haj RA, Dediste A, Vandenberg O. 2012. Comparison of the Microflex LT and Vitek MS systems for routine identification of bacteria by matrix-assisted laser desorption ionizationtime of flight mass spectrometry. J. Clin. Microbiol. 50:1313-1325.
7. Tan KE, Ellis BC, Lee R, Stamper PD, Zhang SX, Carroll KC. 2012. Prospective evaluation of a matrix-assisted laser desorption ionizationtime of flight mass spectrometry system in a hospital clinical microbiology laboratory for identification of bacteria and yeasts: a bench-by-bench study for assessing the impact on time to identification and costeffectiveness. J. Clin. Microbiol. 50:3301-3308.
8. van Veen SQ, Claas EC, Kuijper EJ. 2010. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionizationtime of flight mass spectrometry in conventional medical microbiology laboratories. J. Clin. Microbiol. 48:900-907.
9. Bessede E, Angla-Gre M, Delagarde Y, Sep Hieng S, Menard A, Megraud F. 2011. Matrix-assisted laser-desorption/ionization Biotyper: experience in the routine of a University hospital. Clin. Microbiol. Infect. 17:533-538.
10. Benagli C, Rossi V, Dolina M, Tonolla M, Petrini O. 2011. Matrixassisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria. PLoS One 6:e16424. doi: 10.1371/journal.pone.0016424.
11. Bizzini A, Jaton K, Romo D, Bille J, Prod'hom G, Greub G. 2011. Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains. J. Clin. Microbiol. 49:693-696.
12. Dupont C, Sivadon-Tardy V, Bille E, Dauphin B, Beretti JL, Alvarez AS, Degand N, Ferroni A, Rottman M, Herrmann JL, Nassif X, Ronco E, Carbonnelle E. 2010. Identification of clinical coagulase-negative staphylococci, isolated in microbiology laboratories, by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and two automated systems. Clin. Microbiol. Infect. 16:998-1004.
13. Marko DC, Saffert RT, Cunningham SA, Hyman J, Walsh J, Arbefeville S, Howard W, Pruessner J, Safwat N, Cockerill FR, Bossler AD, Patel R, Richter SS. 2012. Evaluation of the Bruker Biotyper and Vitek MS matrixassisted laser desorption ionization-time of flight mass spectrometry systems for identification of nonfermenting gram-negative bacilli isolated from cultures from cystic fibrosis patients. J. Clin. Microbiol. 50:2034-2039.
14. Justesen US, Holm A, Knudsen E, Andersen LB, Jensen TG, Kemp M, Skov MN, Gahrn-Hansen B, Moller JK. 2011. Species identification of clinical isolates of anaerobic bacteria: a comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems. J. Clin. Microbiol. 49:4314-4318.
15. Mellmann A, Cloud J, Maier T, Keckevoet U, Ramminger I, Iwen P, Dunn J, Hall G, Wilson D, Lasala P, Kostrzewa M, Harmsen D. 2008. Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. J. Clin. Microbiol. 46:1946-1954.
16. Fedorko DP, Drake SK, Stock F, Murray PR. 2012. Identification of clinical isolates of anaerobic bacteria using matrix-assisted laser desorption ionization-time of flight mass spectrometry. Eur. J. Clin. Microbiol. Infect. Dis. 31:2257-2262.
17. Verroken A, Janssens M, Berhin C, Bogaerts P, Huang TD, Wauters G, Glupczynski Y. 2010. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nocardia species. J. Clin. Microbiol. 48:4015-4021.
18. Jamal WY, Shahin M, Rotimi VO. 2013. Comparison of two matrixassisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry methods and API 20AN for identification of clinically relevant anaerobic bacteria. J. Med. Microbiol. 62:540-544.
19. Nagy E, Maier T, Urban E, Terhes G, Kostrzewa M. 2009. Species identification of clinical isolates of Bacteroides by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry. Clin. Microbiol. Infect. 15:796-802.
20. Bizzini A, Greub G. 2010. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. Clin. Microbiol. Infect. 16:1614-1619.
21. McElvania Tekippe E, Shuey S, Winkler DW, Butler MA, Burnham CA. 2013. Optimizing identification of clinically relevant Gram-positive organisms using the Bruker Biotyper MALDI-TOF MS system. J. Clin. Microbiol. 51:1421-1427.
22. Dubois D, Grare M, Prere MF, Segonds C, Marty N, Oswald E. 2012. Performances of the VitekMSmatrix-assisted laser desorption ionizationtime of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology. J. Clin. Microbiol. 50:2568-2576.
23. Cherkaoui A, Hibbs J, Emonet S, Tangomo M, Girard M, Francois P, Schrenzel J. 2010. Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. J. Clin. Microbiol. 48:1169-1175.
24. Funke G, Funke-Kissling P. 2005. Performance of the new VITEK 2 GP card for identification of medically relevant gram-positive cocci in a routine clinical laboratory. J. Clin. Microbiol. 43:84-88.
25. Chatzigeorgiou KS, Sergentanis TN, Tsiodras S, Hamodrakas SJ, Bagos PG. 2011. Phoenix 100 versus Vitek 2 in the identification of grampositive and gram-negative bacteria: a comprehensive meta-analysis. J. Clin. Microbiol. 49:3284-3291.
26. Donay JL, Mathieu D, Fernandes P, Pregermain C, Bruel P, Wargnier A, Casin I, Weill FX, Lagrange PH, Herrmann JL. 2004. Evaluation of the automated phoenix system for potential routine use in the clinical microbiology laboratory. J. Clin. Microbiol. 42:1542-1546.
27. McGregor A, Schio F, Beaton S, Boulton V, Perman M, Gilbert G. 1995. The MicroScan WalkAway diagnostic microbiology system-an evaluation. Pathology 27:172-176.
28. Stefaniuk E, Baraniak A, Gniadkowski M, Hryniewicz W. 2003. Evaluation of the BD Phoenix automated identification and susceptibility testing system in clinical microbiology laboratory practice. Eur. J. Clin. Microbiol. Infect. Dis. 22:479-485.
29. Bizzini A, Durussel C, Bille J, Greub G, Prod'hom G. 2010. Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory. J. Clin. Microbiol. 48:1549-1554.
30. Harris LG, El-Bouri K, Johnston S, Rees E, Frommelt L, Siemssen N, Christner M, Davies AP, Rohde H, Mack D. 2010. Rapid identification of staphylococci from prosthetic joint infections using MALDI-TOF mass-spectrometry. Int. J. Artif. Organs 33:568-574.
31. Carpaij N, Willems RJ, Bonten MJ, Fluit AC. 2011. Comparison of the identification of coagulase-negative staphylococci by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and tuf sequencing. Eur. J. Clin. Microbiol. Infect. Dis. 30:1169-1172.
32. Spanu T, De Carolis E, Fiori B, Sanguinetti M, D'Inzeo T, Fadda G, Posteraro B. 2011. Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to rpoB gene sequencing for species identification of bloodstream infection staphylococcal isolates. Clin. Microbiol. Infect. 17:44-49.
33. Fang H, Ohlsson AK, Ullberg M, Ozenci V. 2012. Evaluation of speciesspecific PCR, Bruker MS, VITEK MS and the VITEK 2 system for the identification of clinical Enterococcus isolates. Eur. J. Clin. Microbiol. Infect. Dis. 31:3073-3077.
34. Gaillot O, Blondiaux N, Loiez C, Wallet F, Lemaitre N, Herwegh S, Courcol RJ. 2011. Cost-effectiveness of switch to matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine bacterial identification. J. Clin. Microbiol. 49:4412.
35. Welker M. 2011. Proteomics for routine identification of microorganisms. Proteomics 11:3143-3153.
36. Sauer S, Freiwald A, Maier T, Kube M, Reinhardt R, Kostrzewa M, Geider K. 2008. Classification and identification of bacteria by mass spectrometry and computational analysis. PLoS One 3:e2843. doi:10.1371/journal.pone.0002843.
37. Welker M, Moore ER. 2011. Applications of whole-cell matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry in systematic microbiology. Syst. Appl. Microbiol. 34:2-11.
38. Wessels E, Schelfaut JJ, Bernards AT, Claas EC. 2012. Evaluation of several biochemical and molecular techniques for identification of Streptococcus pneumoniae and Streptococcus pseudopneumoniae and their detection in respiratory samples. J. Clin. Microbiol. 50:1171-1177.
39. Stevenson LG, Drake SK, Murray PR. 2010. Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 48: 444-447.
40. Ikryannikova LN, Lapin KN, Malakhova MV, Filimonova AV, Ilina EN, Dubovickaya VA, Sidorenko SV, Govorun VM. 2011. Misidentification of alpha-hemolytic streptococci by routine tests in clinical practice. Infect. Genet. Evol. 11:1709-1715.
41. Ferroni A, Suarez S, Beretti JL, Dauphin B, Bille E, Meyer J, Bougnoux ME, Alanio A, Berche P, Nassif X. 2010. Real-time identification of bacteria and Candida species in positive blood culture broths by matrixassisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 48:1542-1548.
42. Davies AP, Reid M, Hadfield SJ, Johnston S, Mikhail J, Harris LG, Jenkinson HF, Berry N, Lewis AM, El-Bouri K, Mack D. 2012. Identification of clinical isolates of alpha-hemolytic streptococci by 16S rRNA gene sequencing, matrix-assisted laser desorption ionization-time of flight mass spectrometry using MALDI Biotyper, and conventional phenotypic methods: a comparison. J. Clin. Microbiol. 50:4087-4090.
43. Werno AM, Christner M, Anderson TP, Murdoch DR. 2012. Differentiation of Streptococcus pneumoniae from nonpneumococcal streptococci of the Streptococcus mitis group by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 50:2863-2867.
44. McElvania Tekippe E, Shuey S, Winkler DW, Butler MA, Burnham CA. 2013. Optimizing identification of clinically relevant gram-positive organisms by use of the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system. J. Clin. Microbiol. 51: 1421-1427.