A camelid antibody candidate for development of a therapeutic agent against Hemiscorpius lepturus envenomation

FASEB Journal

Volumn 28, Issue 9, 2014, Pages 4004-4014

  Yardehnavi, Najmeh ^a   Behdani, Mahdi ^a   Bagheri, Kamran Pooshang ^a   Mahmoodzadeh, Amir ^a   Khanahmad, Hossein ^b   Shahbazzadeh, Delavar ^a   Habibi Anbouhi, Mahdi ^a   Ghassabeh, Gholamreza Hassanzadeh ^c,d   Muyldermans, Serge ^c,d  

^a PASTEUR INSTITUTE OF IRAN   (Iran)

^b ISFAHAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^c Department of Genetics and Molecular Biology Isfahan University of Medical Science ^*  (Belgium)

^d VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

HCAb; Heavy chain antibody; Nanobody; Phage display; Scorpionism

Indexed keywords

F7 NANOBODY; HEMINECROLYSIN; NANOBODY; SCORPION VENOM; SCORPION VENOM ANTISERUM; UNCLASSIFIED DRUG; NEUTRALIZING ANTIBODY; VENOM ANTISERUM;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIDERMONECROTIC EFFECT; ANTIGEN SPECIFICITY; ANTIHEMOLYTIC EFFECT; ARTICLE; ARTIODACTYLA; CAMILLUS DROMEDARIUS; CONTROLLED STUDY; DRUG EFFECT; ENVENOMATION; ERYTHROCYTE; FEMALE; HEMISCORPIUS LEPTURUS; HEMOLYSIS; IMMUNIZATION; IN VITRO STUDY; LETHAL DOSE; MOUSE; NONHUMAN; PHAGE DISPLAY; SCORPION; SCORPIONISM; SERODIAGNOSIS; AMINO ACID SEQUENCE; ANIMAL; BAGG ALBINO MOUSE; CAMEL; CHEMISTRY; DRUG EFFECTS; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOLOGY; METABOLISM; MOLECULAR GENETICS; NECROSIS; PARASITOLOGY; PATHOLOGY; SCORPION STINGS; SKIN DISEASES; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, NEUTRALIZING; ANTIVENINS; BLOTTING, WESTERN; CAMELS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ERYTHROCYTES; FEMALE; HEMOLYSIS; IMMUNIZATION; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; NECROSIS; SCORPION STINGS; SCORPION VENOMS; SCORPIONS; SINGLE-DOMAIN ANTIBODIES; SKIN DISEASES;

EID: 84907222526     PISSN: 08926638     EISSN: 15306860     Source Type: Journal    
DOI: 10.1096/fj.13-247478     Document Type: Article

References (29)

1. Chippaux, J., and Goyffon, M. (2008) Epidemiology of scorpionism: a global appraisal. Acta Trop. 107, 71-79
2. Dehghani, R., and Valaie, N. (2005) The review of status of scorpion sting in Iran and problems from it. Feyz 9, 73-92
3. Borchani, L., Sassi, A., Shahbazzadeh, D., Strub, J.-M., Tounsi-Guetteti, H., Boubaker, M. S., Akbari, A., Van Dorsselaer, A., and El Ayeb, M. (2011) Heminecrolysin, the first hemolytic dermonecrotic toxin purified from scorpion venom. Toxicon 58, 130-139
4. Srairi-Abid, N., Shahbazzadeh, D., Chatti, I., Mlayah-Bellalouna, S., Mejdoub, H., Borchani, L., Benkhalifa, R., Akbari, A., and El Ayeb, M. (2008) Hemitoxin, the first potassium channel toxin from the venom of the Iranian scorpion Hemiscorpius lepturus. FEBS J. 275, 4641-4650
5. Shahbazzadeh, D., Srairi-Abid, N., Feng, W., Ram, N., Borchani, L., Ronjat, M., Akbari, A., Pessah, I., De Waard, M., and El Ayeb, M. (2007) Hemicalcin, a new toxin from the Iranian scorpion Hemiscorpius lepturus which is active on ryanodine-sensitive Ca2+ channels. Biochem. J. 404, 89-96
6. Abderrazek, R. B., Vincke, C., Hmila, I., Saerens, D., Abidi, N., El Ayeb, M., Muyldermans, S., and Bouhaouala-Zahar, B. (2011) Development of Cys38 knock-out and humanized version of NbAahII10 nanobody with improved neutralization of AahII scorpion toxin. Protein Eng. Des. Sel. 24, 727-735
7. Hamers-Casterman, C., Atarhouch, T., Muyldermans, S., Robinson, G., Hammers, C., Songa, E. B., Bendahman, N., and Hammers, R. (1993) Naturally occurring antibodies devoid of light chains. Nature 363, 446-448
8. Muyldermans, S. (2001) Single domain camel antibodies: current status. Rev. Mol. Biol. Technol. 74, 277-302
9. Hmila, I., Saerens, D., Abderrazek, R. B., Vincke, C., Abidi, N., Benlasfar, Z., Govaert, J., El Ayeb, M., Bouhaouala-Zahar, B., and Muyldermans, S. (2010) A bispecific nanobody to provide full protection against lethal scorpion envenoming. FASEB J. 24, 3479-3489
10. World Health Organization (2008) Proposed WHO Guidelines for the production, control and regulation of snake anti-venom immunoglobulins. World Health Organization, Geneva, Switzerland
11. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
12. Al-Badri, Z. M., Som, A., Lyon, S., Nelson, C. F., Nüsslein, K., and Tew, G. N. (2008) Investigating the effect of increasing charge density on the hemolytic activity of synthetic antimicrobial polymers. Biomacromolecules 9, 2805-2810
13. Hoogenboom, H. R., Griffiths, A. D., Johnson, K. S., Chiswell, D. J., Hudson, P., and Winter, G. (1991) Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res. 19, 4133-4137
14. Arbabi Ghahroudi, M., Desmyter, A., Wyns, L., Hamers, R., and Muyldermans, S. (1997) Selection and identification of single domain antibody fragments from camel heavy-chain antibodies. FEBS Lett. 414, 521-526
15. Hmila, I., Abdallah, R. Ba., Saerens, D., Benlasfar, Z., Conrath, K., Ayeb, M. E., Muyldermans, S., and Bouhaouala-Zahar, B. (2008) VHH, bivalent domains and chimeric heavy chain-only antibodies with high neutralizing efficacy for scorpion toxin AahI′. Mol. Immunol. 45, 3847-3856
16. Behdani, M., Zeinali, S., Karimipour, M., ShahrezaH, K., Ghasemi, P., Asadzadeh, N., Ghamnak, A., BagheriK, P., Ahari, H., and Shahbazzadeh, D. (2010) Antiserum production in immunized camel by the venom of Hemiscorpius lepturus scorpion: evaluation of neutralizing test in vivo. Tehran Univ. Med. J. 68, 268-273
17. Behdani, M., Zeinali, S., Khanahmad, H., Karimipour, M., Asadzadeh, N., Azadmanesh, K., Khabiri, A., Schoonooghe, S., Habibi Anbouhi, M., and Hassanzadeh-Ghassabeh, G. (2012) Generation and characterization of a functional nanobody against the vascular endothelial growth factor receptor-2: angiogenesis cell receptor. Mol. Immunol. 50, 35-41
18. Thys, B., Schotte, L., Muyldermans, S., Wernery, U., Hassanzadeh-Ghassabeh, G., and Rombaut, B. (2010) In vitro antiviral activity of single domain antibody fragments against poliovirus. Antiviral Res. 87, 257-264
19. Sambrook, J., and Russell, D. W., eds. (2001) Molecular Cloning: A Laboratory Manual, 3rd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA
20. Lefranc, M.-P., Pommié, C., Kaas, Q., Duprat, E., Bosc, N., Guiraudou, D., Jean, C., Ruiz, M., Da Piédade, I., and Rouard, M. (2005) IMGT unique numbering for immunoglobulin and T cell receptor constant domains and Ig superfamily C-like domains. Dev. Comp. Immunol. 29, 185-203
21. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28, 2731-2739
22. Conrath, K. E., Lauwereys, M., Wyns, L., and Muyldermans, S. (2001) Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs. J. Biol. Chem. 276, 7346-7350
23. F. Bouhaouala-Zahar, B. D., Zenouaki, I., Ben, R. Khalifa, Borchani, L., Pelhate, M. J. C. Boulain, El Ayeb, M., Menez, A., and Karoui, H., (1996) A recombinant insect-specific alpha-toxin of Buthus occitanus tunetanus scorpion confers protection against homologous mammal toxins. Eur. J. Biochem. 238, 553-660
24. Beatty, J. D., Beatty, B. G., and Vlahos, W. G. (1987) Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay. J. Immunol. Methods 100, 173-179
25. Lin, Y., Li, B., Wang, M., Jia, R., Wang, J., Zhang, Y., and Zhu, J. (2011) Improved affinity of a chicken single-chain antibody to avian infectious bronchitis virus by site-directed mutagenesis of complementarity-determining region H3. African J. Biotechnol. 10, 18294-18303
26. Valavi, E., and Ansari, M. A. (2008) Hemolytic uremic syndrome following Hemiscorpius lepturus (scorpion) sting. Indian J. Nephrol. 18, 166
27. Ardekani, L. S., Gargari, S. L. M., Rasooli, I., Bazl, M. R., Mohammadi, M., Ebrahimizadeh, W., Bakherad, H., and Zare, H. (2013) A novel nanobody against urease activity of Helicobacter pylori. Int. J. Infect. Dis.
28. Alvarenga, L. M., Martins, M. S., Moura, J. F., Kalapothakis, E., Oliveira, J. C., Mangili, O. C., Granier, C., and Chávez-Olórtegui, C. (2003) Production of monoclonal antibodies capable of neutralizing dermonecrotic activity of Loxosceles intermedia spider venom and their use in a specific immunometric assay. Toxicon 42, 725-731
29. Vincke, C., and Muyldermans, S. (2012) Introduction to heavy chain antibodies and derived nanobodies. In Single Domain Antibodies: Methods and Protocols (Saerens, D., and Muyldermans, S., eds) pp. 15-26, Springer, New York