Fenugreek extract diosgenin and pure diosgenin inhibit the hTERT gene expression in A549 lung cancer cell line

Molecular Biology Reports

Volumn 41, Issue 9, 2014, Pages 6247-6252

  Rahmati Yamchi, Mohammad ^a,b   Ghareghomi, Somayyeh ^c   Haddadchi, Gholamreza ^c   Milani, Morteza ^d   Aghazadeh, Mohammad ^b   Daroushnejad, Hasan ^d  

^a TABRIZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b TABRIZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^c GOLESTAN UNIVERSITY   (Iran)

^d TABRIZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Diosgenin; Fenugreek; Lung cancer; MTT assay; pTLC; qRT PCR; Telomerase

Indexed keywords

DIOSGENIN; FENUGREEK EXTRACT; FENUGREEK EXTRACT DIOSGENIN; PLANT EXTRACT; PURE DIOSGENIN; TELOMERASE REVERSE TRANSCRIPTASE; UNCLASSIFIED DRUG; COMPLEMENTARY DNA; FENUGREEK SEED MEAL; RNA; TELOMERASE; TERT PROTEIN, HUMAN;

A549 CELL LINE; ANTIDIABETIC ACTIVITY; ANTINEOPLASTIC ACTIVITY; ANTINOCICEPTION; ARTICLE; CONTROLLED STUDY; DOWN REGULATION; DRUG DETERMINATION; DRUG IMPURITY; DRUG INHIBITION; DRUG PURITY; DRUG STRUCTURE; FENUGREEK; GENE EXPRESSION; HTERT GENE; HYPOCHOLESTEROLEMIA; IC 50; MTT ASSAY; NONHUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; TRADITIONAL MEDICINE; CHEMISTRY; DRUG EFFECTS; GENE EXPRESSION REGULATION; GENETICS; HUMAN; IC50; LUNG TUMOR; METABOLISM; PHYTOTHERAPY; PLANT SEED; TRIGONELLA; TUMOR CELL LINE;

TRIGONELLA FOENUM-GRAECUM;

CELL LINE, TUMOR; DIOSGENIN; DNA, COMPLEMENTARY; DOWN-REGULATION; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; INHIBITORY CONCENTRATION 50; LUNG NEOPLASMS; PHYTOTHERAPY; PLANT EXTRACTS; RNA; SEEDS; TELOMERASE; TRIGONELLA;

EID: 84906934929     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-014-3505-y     Document Type: Article

References (23)

1. Blackburn E, Collins K (2011) Telomerase: an RNP enzyme synthesizes DNA. Cold Spring Harb Perspect Biol 3:a003558
2. Maraei AM, Hatta AZ, Shiran MS et al (2012) Human telomerase reverse transcriptase expression in ovarian tumors. Indian J Pathol Microbiol 55:187-191
3. Athey V, Suckling R, Tod A et al (2012) Early diagnosis of lung cancer: evaluation of a community-based social marketing intervention. Thorax 67:412-417
4. Herbst RS, Heymach JV, Lippman SM (2008) Lung cancer. N Engl J Med 359:1367-1380
5. Altuntas E, EnginOzgoz O, Taser F (2005) Some physical properties of fenugreek (Trigonella foenum-graecum) seeds. J Food Eng 71:37-43
6. Srinivasan K (2006) Fenugreek (Trigonella foenum-graecum): a review of health beneficial physiological effects. Food-Rev Int 22:203-224
7. Madhava M, Shyamala BN, Pura J et al (2010) Chemical composition and antioxidant activity of husk and endosperm of fenugreek seeds. LWT-Food Sci Technol 44:451-456
8. Nagore D, Patil P, Kuber V (2012) Comparison between high performance liquid chromatography and high performance thin layer chromatography determination of diosgenin from fenugreek seeds. Int J Green Pharm 6:315-320
9. Miyoshi N, Nagasawa T, Mabuchi R et al (2011) Chemoprevention of azoxymethane/dextran sodium sulfate-induced mouse colon carcinogenesis by freeze-dried Yam Sanyaku and its constituent diosgenin. Cancer Prev Res 4:924-934
10. Kaviarasan S, Vijayalakshmi K, Anuradha CV (2007) A Polyphenol-rich extract of fenugreek seeds protect erythrocytes from oxidative damage. Plant Foods Hum Nutr 59:143-147 (Pubitemid 40172001)
11. Amin A, Alkaabi A, Al-Falasi S et al (2005) Chemopreventive activities of Trigonella foenum-graecum (Fenugreek) against breast cancer. Cell Biol Int 29:687-694 (Pubitemid 41350751)
12. SujaPandian R, Anuradha VV, Viswanathan P (2002) Gastroprotective effect of fenugreek seeds (Trigonella foenum-graecum) on experimental gastric ulcer in rats. J Ethnopharmacol 81:393-397
13. Zia T, NazrulHasnain S, Hasan SK (2001) Evaluation of the oral hypoglycaemic effect of Trigonella foenum-graecum lin normal mice. J Ethnopharmacol 75:191-195
14. Raju J, Patlolla JM, Swamy MV, Swamy M et al (2004) Diosgenin, a steroid saponin of Trigonella foenum-graecum (Fenugreek), inhibits azoxymethane-induced aberrant crypt foci formation in F344 rats and induces apoptosis in HT-29 human colon cancer cells. Cancer Epidemiol Biomarkers Prev 13:1392-1398 (Pubitemid 39071566)
15. Shishodia S, Aggarwal BB (2006) Diosgenin inhibits osteoclastogenesis, invasion, and proliferation through the down regulation of Akt, I kappa B kinase activation and NF-jB regulated gene expression. Oncogene 25:1463-1473 (Pubitemid 43361932)
16. Corbiere C, Liagre B, Terro F et al (2004) Induction of antiproliferative effect by diosgenin through activation of p53, release of apoptosis-inducing factor (AIF) and modulation of caspase-3activity in different human cancer cells. Cell Res 14:188-196 (Pubitemid 43231816)
17. Li F, Fernandez PP, Rajendran P (2010) Diosgenin, a steroidal saponin, inhibits SATA3 signaling pathway leading to suppression of proliferation and chemosensitization of human hepatocellular carcinoma cells. Cancer Lett 292:197-207
18. Chen P, Shih YW, Huang H et al (2011) Diosgenin, a steroidal saponin, inhibits migration and invasion of human prostate cancer PC-3 cells by reducing matrix metalloproteinases expression. PLoS One 6:20164
19. Srinivasan S, Koduru S, Kumar R et al (2009) Diosgenin targets Akt-mediated prosurvival signaling in human breast cancer cells. Int J Cancer 125:961-967
20. Rahmati M, Ghareghomi S, Haddadchi G et al (2013) Diosgenin inhibits hTERT gene expression in the A549 lung cancer cell line. Asian Pac J Cancer Prev 14:6945-6948
21. Nasiri M, Zarghami N, Nejati K et al (2013) Curcumin and silibinin inhibit telomerase expression in T47d human breast cancer cells. Asian Pacific J Cancer Prev 14:3449-3453
22. Philip CJ, Giardina CK, Bilir B, Cutler D et al (2012) Genistein cooperates with the histonedeacetylase inhibitor vorinostat to induce celldeath in prostate cancer cells. BMC Cancer 12:145
23. Tang XH, Gudas LJ (2011) Retinoids, retinoic acid receptors, and cancer. Annu Rev Pathol 6:345-364